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Glibenclamide, gliclazide and glipizide triple test card and test method thereof

The technology of a glipizide triple detection card and glibenclamide is applied in the direction of measuring devices, test sample preparation, instruments, etc., which can solve the problems of cumbersome operation and high professional requirements, and achieve accurate results, easy preparation, and saving The effect of testing costs

Inactive Publication Date: 2011-04-13
长沙安迪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a colloidal gold test paper that is easy and quick to operate, low in cost, easy to carry, can be operated by non-professionals, and can be detected on-site, and can detect three types of hypoglycemic components of western medicine at the same time, overcoming the operation of the prior art The defects of cumbersome and high professional requirements

Method used

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  • Glibenclamide, gliclazide and glipizide triple test card and test method thereof
  • Glibenclamide, gliclazide and glipizide triple test card and test method thereof
  • Glibenclamide, gliclazide and glipizide triple test card and test method thereof

Examples

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Embodiment 1

[0026] Example 1: Triple detection card for glibenclamide, gliclazide and glipizide

[0027] It is to set the test strip 2 in the test card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 7cm long, 2cm wide, 0.5cm thick, and the thin shell wall thickness is 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0028] The test strip 2 is a thin strip with a multilayer structure, the length of the strip is about 6.0cm, the width of the strip is about 0.4cm, and the thickness is less than 2.5mm. The test strip 2 has a multilayer structure: the bottom layer is a supporting backboard 5, wh...

Embodiment 2

[0030] The glibenclamide, gliclazide and glipizide triple detection card of the present invention is used to detect whether glibenclamide, gliclazide and glipizide are contained in hypoglycemic traditional Chinese medicine samples such as capsules, tablets and pills Based on this, it is possible to identify whether there is any illegal addition of western medicine hypoglycemic ingredients in Chinese medicines for hypoglycemia.

[0031] The processing method is as follows:

[0032] (1) Randomly collect a certain amount of samples to be tested and crush them evenly;

[0033] (2) Weigh 5g of the pulverized sample, add 10mL of sample extract, stir and mix for more than 5 minutes, and shake for 30 minutes;

[0034] The extract solution is configured as follows: add 30mL distilled water to 70mL methanol;

[0035](3) Transfer the solution obtained in step (2) into a 10mL centrifuge tube, centrifuge at 4000r / min for 10min, and take the supernatant after centrifugation; the supernata...

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Abstract

The invention relates to a glibenclamide, gliclazide and glipizide triple test card and a test method thereof, belonging to the technical field of testing of western medicine components which are illegally added into traditional Chinese medicines. A test bar is arranged in a shell of the triple test card and comprises a sample pad, a plurality of sections of colloidal gold membranes, a nitrocellulose membrane and a water-absorbing membrane which are sequentially stuck on a supporting backboard, wherein the plurality of sections of the colloidal gold membranes are glass fiber membranes containing colloidal gold markers of an anti-glibenclamide antibody, an anti-gliclazide antibody and an anti-glipizide antibody sequentially, three test strips which respectively contain a glibenclamide protein conjugate, a gliclazide protein conjugate and a glipizide protein conjugate are arranged on the nitrocellulose membrane, and the triple test card additionally comprises a quality control strip containing an anti-rabbit antibody or an anti-mouse antibody. The triple test card has the advantages of being capable of simultaneously detecting glibenclamide, gliclazide and glipizide which are illegally added in a glucose-lowering traditional Chinese medicine. The test card is easy for preparation, convenient and fast for use and accurate in result, and can be used for saving the test cost.

Description

technical field [0001] The invention relates to a device for detecting illegally added western medicine ingredients in traditional Chinese medicine, in particular to a triple detection card of glibenclamide, gliclazide and glipizide and a detection method thereof, belonging to the technical field of detection of illegally added western medicine ingredients in traditional Chinese medicine . Background technique [0002] Glibenclamide, Gliclazide and Glipizide are the most commonly illegally added hypoglycemic Western drug ingredients in diabetes-lowering traditional Chinese medicine or health care products. According to the sampling inspection conducted by five provinces and cities on 32 kinds of traditional Chinese medicines and traditional Chinese medicine health products that claim to have hypoglycemic effects on the market, it is found that 70% of the medicines have hypoglycemic western medicine ingredients added. If a patient takes an overdose of a so-called pure Chines...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N1/28
Inventor 周坚吴文晔熊晓芳
Owner 长沙安迪生物科技有限公司
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