Developing solution for immunochromatography, and measurement method using same

A technique of immunochromatography and developing solution, which is applied in the field of determination of substances to be detected, can solve the problems of not being able to obtain positive signals, and achieve the effect of inhibiting non-specific reactions and improving stability.

Inactive Publication Date: 2011-05-18
TANAKA PRECIOUS METAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, when an insoluble carrier is used as a labeling substance, a positive signal corresponding to the amount of the insoluble carrier captured at the reaction site cannot be obtained, especially when a small volume of insoluble carrier is used.

Method used

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  • Developing solution for immunochromatography, and measurement method using same
  • Developing solution for immunochromatography, and measurement method using same
  • Developing solution for immunochromatography, and measurement method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] 1. Preparation of Reaction Sites onto Chromatographic Media

[0076] Using an antibody applicator (manufactured by BioDot), apply an anti-influenza virus B monoclonal antibody diluted to a concentration of 1.0 mg / mL with a phosphate buffer solution (pH 7.4) containing 5% by weight isopropanol to 25×2.5 cm nitrocellulose membrane (manufactured by Millipore: HF 120), and dried at 50° C. for 30 minutes. After drying, the nitrocellulose membrane was soaked at 30° C. for 30 minutes in 200 mL of a phosphate buffer solution (pH 7.4) containing 0.5% by weight of casein (manufactured by Wako Junpharma Co., Ltd.) to block. After blocking, it was washed with a washing solution containing 0.05% by weight of Tween 20, dried overnight at room temperature, and the reaction site was prepared on a chromatographic medium.

[0077] 2. Preparation of Labeling Substance Solution

[0078] Add 0.1 mL of anti-influenza virus B monoclonal antibody diluted to a concentration of 0.1 mg / mL with ...

Embodiment 2

[0092] Using the chromatographic medium prepared in Example 1, as a developing solution, except that 0.3% by weight of Triton X-100 was used instead of Tween 20, other measurements were performed in the same manner as in Example 1. The results are shown in Table 1.

Embodiment 3

[0098] Using the chromatographic medium prepared in Example 1, as a developing solution, except that 0.05% by weight of Tween 20 and 0.3% by weight of Triton X-100 were used instead of 0.5% by weight of Tween 20, other measurements were performed in the same manner as in Example 1. The results are shown in Table 1.

[0099] Table 1

[0100] closed

[0101]

[0102] Comparing the above-mentioned Examples 1-3 with Comparative Examples 1-3 shows that in the presence of a nonionic surfactant and PVP as an ethylenic water-soluble polymer having a polar group containing an oxygen atom, high sensitivity can be obtained. The detection was from the nucleoprotein of influenza virus B. On the other hand, when sodium carboxymethylcellulose (CMC·Na) or polyethylene glycol was used as the water-soluble polymer, non-specific adsorption was found also in negative test samples (Comparative Examples 4 and 5). When the ionic surfactant sodium cholate was used together with PVP, detection w...

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Abstract

Provided is a developing solution for immunochromatography that is precise and highly sensitive and is able to sufficiently detect even a low-concentration substance to be detected while controlling false positive reactions caused by non-specific adsorption of components other than the substance to be detected contained in a sample. The invention relates to a developing solution that contains a non-ionic surfactant and a vinyl-based water-soluble polymer having oxygen atom- and nitrogen atom-containing polar groups, for immunochromatography using a detection reagent labeled with an insoluble carrier.

Description

technical field [0001] The present invention relates to a developing solution constituting a mobile phase in immunochromatography as one of immunoassay methods. In addition, the present invention also relates to an assay method capable of highly sensitively detecting an analyte contained in a biological sample using the developing solution. Background technique [0002] In the immunoassay method that utilizes the specific reaction between an antigen and its corresponding antibody and detects a substance to be detected by a specific antigen or antibody, the substance to be detected in the sample is combined with the sensitized antibody that is bound to the particle for detection Or the agglutination method of antigen binding through immune reaction and measuring the agglutination state of the resulting detection particles is recognized as a simple method, especially because the results can be judged visually, making it a commonly used method. [0003] In addition, a method i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543G01N33/553
CPCG01N33/533Y10T436/107497
Inventor 伊藤大辅
Owner TANAKA PRECIOUS METAL IND
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