Application of human steam cell growth factors in cosmetics
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A growth factor, stem cell technology, used in cosmetics, cosmetics, cosmetic preparations, etc.
Active Publication Date: 2011-05-25
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Embodiment 1
[0064] Acquisition of Human Stem Cell Growth Factor
[0065] Total RNA was extracted from human umbilical cord mesenchymal stem cells and reverse transcribed into cDNA. PCR primers were designed according to the full-length human SCGF gene sequence (GenBank Accession No. NM_002975). SCGF1 upstream primer sequence: 5'-CAAGAGTCCAGCTTAATGCAGGCAG-3', SCGF1 downstream primer sequence: 5'-GCTAGAAGGGGAACTCGCAGACGTA-3'. The above obtained CDNA was used as a template, and the SCGF1 gene was obtained by PCR. SCGF mature peptide coding sequence (SCGF2) PCR primer (GenBank: AB009244.1) was designed: upstream of SCGF2: 5′-ATTTAAAACATATGGGTGCTCGGGGAGCAGAGAG-3′, downstream of SCGF2: 5′-ATTGGATCCCTAGAAGGGGAACTCGCAG-3′. SCGF2 was obtained by PCR reaction using SCGF1 as a template. Digest SCGF2 and pET-28a(+) plasmids with Nde I and BamH I, connect them with T4 DNA ligase, transform the adapters into competent Escherichia coli DH5α, and transform the recombinant plasmid pET-28a-SCGF into Esc...
Embodiment 2
[0067] The ingredients of the hand cream are grouped, as shown in Table 1, three formulations of hand cream containing human stem cell growth factor are prepared:
[0068]
[0069] Preparation process: Dissolve component A at 70-80°C, add component B to homogenize (negative pressure, homogenize 1-2 times, 15-30 minutes each time) to emulsify, cool down to 37-50°C, add C, Component D, stirred evenly, and vacuum degassed to obtain three kinds of hand creams containing human stem cell growth factor.
Embodiment 3
[0071] Stem Cell Growth Factor Eye Cream
[0072] The eye cream ingredients were grouped, as shown in Table 2, and three formulations of eye cream containing human stem cell growth factor were prepared:
[0073]
[0074] Process: Heat and dissolve component B with part of deionized water, stir evenly, dissolve component A at 70-80°C, pour component A into component B, stir evenly, cool down to 37-50°C, add C, D component, just stir well.
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Abstract
The invention provides application of human steam cell growth factors in cosmetics. In an application method, human cell growth factors are added into the cosmetics by a homogenizing process, wherein the human steam cell growth factor content in each gram of cosmetics is between 0.1 and 20.0 ng, and the purity is between 85 and 99 percent. The specific application method comprises the following steps of: dividing components which need to be heated in raw materials of the cosmetics into an oil-based component and a water-based component, heating respectively, and mixing; and homogenizing and emulsifying, cooling to the temperature of between 37 and 50 DEG C, adding solution containing the human steam cell growth factors and components which do not need to be heated, stirring uniformly, and performing vacuum degassing. In the application method, the human steam cell growth factors are applied to the cosmetics for the first time, the human steam cell growth factors still can keep excellent activity in a cosmetic matrix material, and the wrinkle resistance of the cosmetics containing the human steam cell growth factors is still high after the cosmetics are stored for one month.
Description
technical field [0001] The invention particularly relates to the application of a human stem cell growth factor in cosmetics, which belongs to the field of daily chemical products. Background technique [0002] The skin is subdivided into the superficial and thinner epidermis, the more fibrous dermis, and the subcutaneous tissue. The epidermis is differentiated from the ectoderm that covers the surface of the embryo and is composed of multiple layers of squamous epithelium that vary in thickness from the thickest scalp to the thinnest eyelid skin (0.04mm) throughout the body. The epidermis can be subdivided into keratinocytes, dendritic cells, and Michael cells; the dermis is mainly developed from the mesoderm, located under the epidermis, and separated from the epidermis by a layer of basement membrane. The dermis is mainly composed of connective tissue, including blood vessels, nerves, muscles, lymph, sweat glands, and sebaceous glands. The shallower layer is called the p...
Claims
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Application Information
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