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Detection reagent for lipoprotein-associated phospholipase A2, preparation method and determination method

A technology for detecting reagents and phospholipases, which is applied to measuring devices, instruments, scientific instruments, etc., and can solve problems such as poor repeatability, high cost, and radioactive contamination of reagents

Inactive Publication Date: 2022-04-26
深圳市三励方科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High performance liquid chromatography has low sensitivity and is easily interfered by various components in blood
The radioactivity measurement method has problems such as radioactive contamination of reagents, low accuracy, and poor repeatability; the enzymatic hydrolysis substrate method mainly relies on imported reagents, which has problems such as high cost

Method used

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  • Detection reagent for lipoprotein-associated phospholipase A2, preparation method and determination method
  • Detection reagent for lipoprotein-associated phospholipase A2, preparation method and determination method
  • Detection reagent for lipoprotein-associated phospholipase A2, preparation method and determination method

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Embodiment Construction

[0046]The following description serves to disclose the present invention to enable those skilled in the art to carry out the present invention. The preferred embodiments described below are only examples, and those skilled in the art can devise other obvious variations.

[0047] A detection reagent for lipoprotein-associated phospholipase A2, which is composed of reagent R1, reagent R2 and reagent R3 that are packaged independently of each other. Reagent R1 is formed by dissolving ethylenediaminetetraacetic acid in R1 buffer solution with a pH value of 7.6 Solution, reagent R2 is the R2 buffer solution with a pH value of 7.2, and reagent R3 is 1-myristoyl-2-(4-nitrophenylsuccinyl)-sn-propanetriyl-3-phosphocholine dissolved in a pure A solution formed in 99% ethanol;

[0048] The chemical formula of 1-myristoyl-2-(4-nitrophenylsuccinyl)-sn-glyceryl-3-phosphocholine is as follows:

[0049]

[0050] R1 buffer solution is 150mmol / L sodium chloride, 20mmol / L ethylenediaminetet...

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Abstract

The invention discloses a detection reagent preparation and determination method for lipoprotein-associated phospholipase A2, and relates to the field of in-vitro detection reagents, the detection reagent is composed of a reagent R1, a reagent R2 and a reagent R3 which are mutually independently subpackaged, the reagent R1 is a buffer solution with the pH value of 7.6, the reagent R2 is a buffer solution with the pH value of 2.7, and the reagent R3 is a buffer solution with the pH value of 2.7. The reagent R3 is a solution formed by dissolving 1-myristoyl-2-(4-nitrophenyl succinyl)-sn-propyl-3-phosphorylcholine in absolute ethyl alcohol, and the reagent R3 is a solution formed by dissolving 1-myristoyl-2-(4-nitrophenyl succinyl)-sn-propyl-3-phosphorylcholine in The detection reagent disclosed by the invention has the advantages that the detection reagent which is based on a continuous monitoring method and takes the 1-myristoyl-2-(4-nitrophenyl succinyl)-sn-propyl-3-phosphorylcholine as a substrate is provided, and the lipoprotein-related phospholipase A2 in a sample can be rapidly and accurately detected.

Description

technical field [0001] The invention relates to the field of in vitro detection reagents, in particular to a detection reagent, preparation and determination method of lipoprotein-related phospholipase A2. Background technique [0002] Lipoprotein-associated phospholipase A2 (Lipoprotein-associated phospholipase A2 (Lp-PLA2)), also known as platelet-activating factor acetylhydrolase (PAF-AH), is a calcium-binding enzyme released by inflammatory cells in atherosclerotic plaques. Unrelated phospholipase, in the circulatory system, lipoprotein-associated phospholipase A2 mainly binds to low-density lipoprotein particles, while only a small part of the enzyme binds to high-density lipoprotein, and lipoprotein-associated phospholipase A2 hydrolyzes oxidized low-density lipoprotein Generates two pro-atherogenic and pro-inflammatory complexes: lysoacylcholine and oxidized free fatty acids. Both substances are highly likely to play an important role in the formation of vulnerable a...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/06
CPCG01N30/90G01N30/06
Inventor 李敏林明吉唐晓玲余金虾周文峰
Owner 深圳市三励方科技有限公司
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