Method and means for altering fiber strength in fiber-producing plants
A fiber strength, fiber technology, applied in the agricultural field, can solve the problem of not producing spinnable fibers
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Embodiment approach
[0068] The present invention is based on the unexpected discovery that G. hirsutum orthologs at the fiber strength locus on chromosome A05 in G. hirsutum plants have an orthologous The presence of homologues results in increased fiber strength of the upland cotton plant.
[0069] Thus, in a first aspect, the present invention provides non-naturally occurring cotton plants, parts and progeny thereof, comprising at least one superior allele of a quantitative trait locus (QTL) for fiber strength located on chromosome A05.
[0070] As used herein, the term "non-naturally occurring" or "cultivated" when used in reference to a plant means a plant having a genome that has been modified by man. A transgenic fiber-producing plant is, for example, a non-naturally occurring fiber-producing plant that contains an exogenous nucleic acid molecule, for example, a chimeric gene comprising a transcribed region that, when transcribed, produces a gene capable of reducing expression of the GLUC g...
Embodiment 2
[0308]Column 2 lists the markers linked to the intensity locus on chromosome A05. Markers shown as CIRx, NAUx and BNLx are publicly available markers (see Cotton Microsatellite Database at http: / / www.cottonmarker.org / ). The marker shown as 'Fragment Size Amplified by Primer Combination X and Y' is the AFLP marker (Vos et al., 1995, NAR 23:4407-4414). The first column marks their map positions on the genetic map (expressed in cM) of the FMBC1F1 mapping population constructed using JoinMap software mapping version 8. BC4F2 lines 6, 10, 20 and 94 generated on BC4F1 plants indicate the graphical genotypes for the markers: a = homozygous FM966, h = heterozygous. Segregation of the 'h' region in this illustrated genotype was investigated using marker data for markers marked with *. The mean phenotypic performance of fiber strength was compared for the group of plants homozygous for the FM966 marker (genotype "HH FM") and the group of plants homozygous for the Pima marker (genotype...
Embodiment 3
[0314] DNA sequencing spanning approximately 165 kb and 136 kb regions covering the upland cotton GLUC1.1A (SEQ ID NO: 53) and GLUC1.1D alleles (not shown), respectively, was performed using 454 DNA Sequencing (454Life Sciences): first using GLUC1.1 Portions of the gene were used as probes against the FMBAC library, and BAC clones with genomic DNA spanning each GhGLUC1.1 allele were identified by hybridization. A number of BAC clones were isolated, confirmed by PCR and grouped into alleles. Selected BAC clones were sequenced to define neighboring genes, which was easily performed by bioinformatics annotation software programs and EST retrieval (see Figure 9 ). The BAC sequence data also identified an additional molecular marker (CIR280) located on an adjacent gene (HAT) (see Tables 6 and 7 for estimated positions on chromosome A05 in the FM BC1 population). Example 3: Analysis of the biological role of glucanases in fiber strength 3.1 Determination of the link between inact...
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