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Biochip and preparation method thereof

A technology of biological chips and biological substances, which is applied in the fields of biochemical equipment and methods, biological testing, and microbial determination/inspection, etc., to achieve the effects of simple preparation process, wide application range and low cost

Inactive Publication Date: 2011-06-01
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no patents for biological applications of stable dispersion colloids containing metal nanoparticles in aqueous solution for inkjet printing.

Method used

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  • Biochip and preparation method thereof
  • Biochip and preparation method thereof
  • Biochip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of HeLa cell chip

[0039] Prepare the ink-jet ink soluble ink containing 2nm gold nanoparticles, the specific preparation method is as follows:

[0040] 1) First prepare a polymer-protected stable gold nanoparticle dispersion with a particle size of 2nm, remove excess polymer protective agent by dialysis, freeze-dry, and prepare a 10% mass concentration with double-distilled water after autoclaving Gold nanoparticles solution. Before adding the polymer dispersant, the toxic effect of gold nanoparticles on HeLa cells was detected by MTT colorimetry, and the results are shown in figure 1 . Depend on figure 1 It can be seen that with the increase of co-cultivation time, the percentage of cell viability decreased. It is proved that gold nanoparticles have a strong ability to inhibit the growth of cells and a great toxicity to cells. According to the principle of chip selection, it was demonstrated that gold nanoparticles can be used.

[...

Embodiment 2

[0044] Embodiment 2, preparation of K-562 cell chip template

[0045] Prepare the ink-jet ink soluble ink containing silver nanoparticles with a particle size of 10nm, the specific preparation method is as follows:

[0046] 1) First prepare the silver nanoparticle dispersion of the stable particle size 10nm of macromolecule protection, after dialysis removes excess macromolecule protective agent, freeze-dry, be mixed with the mass concentration of 18% with double distilled water after autoclaving solution of silver nanoparticles. Before adding the polymer dispersant, the toxic effect of silver nanoparticles on K-562 cells was detected by MTT colorimetry, and the results are shown in figure 1 . Prove that silver nanoparticles can be used.

[0047] 2) Add 2 mL of hexanediol as a co-solvent in 0.80 g of butylene and styrene copolymer (macromolecule dispersant), after dissolving completely, add the silver nanoparticle solution with a mass concentration of 18% prepared above 4 ...

Embodiment 3

[0050] Embodiment 3, the preparation of ESF cell chip template

[0051] Prepare the inkjet ink soluble ink containing platinum nanoparticles with a particle size of 25nm, the specific preparation method is as follows:

[0052] 1) First prepare the stable particle diameter 25nm platinum nanoparticle dispersion of macromolecule protection, dialyze to remove excess polymer protective agent, freeze-dry, and prepare the mass concentration of 8% with double distilled water after autoclaving Platinum nanoparticles solution. Before adding the polymer dispersant, the toxic effect of platinum nanoparticles on ESF cells was detected by MTT colorimetry, and the results are shown in figure 1 . Prove that platinum nanoparticles can be used.

[0053] 2) First add 2 mL of glycerol to 0.80 g of gelatin and glutaraldehyde cross-linked compound (polymer dispersant). After the dissolution is complete, add 4.0 mL of platinum nanoparticle solution with a mass concentration of 8% to the solution ...

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Abstract

The invention discloses a biochip and a preparation method thereof. The preparation method of the biochip comprises the following steps of: printing a micro-nano metal array pattern on a substrate by using metal nanoparticle-containing ink-jet water-soluble ink through an ink jet printer; curing, performing sterilization and disinfection treatment on the substrate with the array pattern, and then co-culturing the substrate with a biological material to obtain the biochip, wherein a preparation method of the jet-ink water-soluble ink comprises the following steps of: mixing metal nanoparticles, a water-soluble cosolvent, a high-molecular dispersing agent, a surfactant and secondary distilled water with stirring; pre-dispersing; and concentrating to obtain the ink. The chip prepared by the method is lower in cost; a preparation process is simple, and is easy to master and convenient to popularize; the biochip is quickly detected and is environmentally-friendly; and compared with similar products at home and abroad, the biochip has the advantages of obvious performance, low cost and the like. The biochip has a wide application range, provides great help for clinic diagnosis and epidemiological screening, and has a broad application prospect in the aspect of scientific research.

Description

technical field [0001] The invention relates to a biological chip and a preparation method thereof. Background technique [0002] Biochips mainly refer to microarrays composed of biologically active substances (including cells, nucleic acids, proteins, and microtissues) assembled on solid substrates to realize the detection of compounds (including drugs), proteins, nucleic acids, cells, and other biological components. Accurate, rapid and informative selective growth, enrichment, screening or detection. [0003] The arrangement of a large number of cells is extremely important for the study of cell-cell interactions. For example, two cell populations (or two single cells) can be located in two regions separated by a certain distance, and then the relationship between them can be studied. interaction and material exchange. The significance of cell arrangement is also reflected in the ease of parallel observation of the physiological conditions of a large number of cells, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12Q1/02C12Q1/68G01N33/53
Inventor 江龙崔文娟李津如荣惠林张雅坤鲁闻生林官华
Owner INST OF CHEM CHINESE ACAD OF SCI
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