Method for evaluating quality of Chinese patent medicament by using metabonomics

Abstract

The invention provides a method for evaluating the quality of a Chinese patent medicament by using metabonomics. The method comprises the following steps: 1) according to the indication of traditional Chinese medicines, establishing a corresponding animal model and screening; 2) taking various of samples of the traditional Chinese medicines, and intervening a disease model animal by a corresponding route of administration; 3) collecting a corresponding biological sample and acquiring the metabolism finger-print of the sample; 4) carrying out pattern recognition on the metabolism fingerprints of a normal group and a disease group to obtain a metabolism biomarker group; 5) calculating the similarity of the metabolism biomarker group and the normal group in different administration groups, or carrying out PLS (partial least squares) regression analysis on differential endogenous metabolites, and observing the influence of medicament treatment on the endogenous metabolites; and 6) according to the similarity result or the result of the PLS regression analysis, evaluating the medicaments, wherein the more normal the control group trends to be, the better efficacy of the medicament is.

Description

technical field [0001] The invention belongs to a method for controlling and evaluating the quality of traditional Chinese medicines, in particular adopting biological fingerprints to control and evaluate the quality of traditional Chinese medicines, and mainly adopting technologies and strategies related to metabolomics for research. Background technique [0002] Traditional Chinese medicine is a treasure of the Chinese nation and is known as the fifth greatest invention in ancient China. Traditional Chinese medicine has attracted widespread attention from all over the world due to its rich resources, unique curative effect, and less toxic and side effects. In today's world, with the rise of the "return to nature" trend of thought, the voice of looking for natural medicines is increasing. Facing the transformation of medical models, the competition in the international market of traditional medicine, and the protection of intellectual property rights after China's accession...

AI Technical Summary

Problems solved by technology

Although the chemical fingerprint of traditional Chinese medicine considers as many components as possible, it can promote the quality consistency and stability of the product to a certain extent, but it is also difficult to reflect its safety and effectiveness. stage has not been effectively expressed

In addition, the chemical fingerprint of traditional Chinese medicine is only the "chemical barcode" of some components of traditional Chinese medicine, which cannot fully control all components. A considerable number of pharmaceutical components are difficult to detect with conventional chromatographic or spectral methods. The repeatability, specificity and Representativeness, etc. need to be further studied

[0004] Therefore, although the existing quality control mode of traditional Chinese medicine has played an important role in ensuring the effectiveness, safety and quality of traditional Chinese medicine and its products, it is not enough to explain the overall effect of traditional Chinese medicine and the multi-component and multi-target effects. synergistic features

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