Method for evaluating quality of Chinese patent medicament by using metabonomics

A technology of quality evaluation and metabolomics, applied in the field of quality control and evaluation of traditional Chinese medicine, can solve problems such as inability to control components, difficulty reflecting safety and effectiveness, insufficient explanation of multi-component and multi-target synergy characteristics of traditional Chinese medicine, and achieve Ensure safe and effective, stable and controllable internal quality

CN102100706AInactive Publication Date: 2011-06-22DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2011-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for evaluating the quality of a Chinese patent medicament by using metabonomics. The method comprises the following steps: 1) according to the indication of traditional Chinese medicines, establishing a corresponding animal model and screening; 2) taking various of samples of the traditional Chinese medicines, and intervening a disease model animal by a corresponding route of administration; 3) collecting a corresponding biological sample and acquiring the metabolism finger-print of the sample; 4) carrying out pattern recognition on the metabolism fingerprints of a normal group and a disease group to obtain a metabolism biomarker group; 5) calculating the similarity of the metabolism biomarker group and the normal group in different administration groups, or carrying out PLS (partial least squares) regression analysis on differential endogenous metabolites, and observing the influence of medicament treatment on the endogenous metabolites; and 6) according to the similarity result or the result of the PLS regression analysis, evaluating the medicaments, wherein the more normal the control group trends to be, the better efficacy of the medicament is.
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Description

technical field

[0001] The invention belongs to a method for controlling and evaluating the quality of traditional Chinese medicines, in particular adopting biological fingerprints to control and evaluate the quality of traditional Chinese medicines, and mainly adopting technologies and strategies related to metabolomics for research. Background technique

[0002] Traditional Chinese medicine is a treasure of the Chinese nation and is known as the fifth greatest invention in ancient China. Traditional Chinese medicine has attracted widespread attention from all over the world due to its rich resources, unique curative effect, and less toxic and side effects. In today's world, with the rise of the "return to nature" trend of thought, the voice of looking for natural medicines is increasing. Facing the transformation of medical models, the competition in the international market of traditional medicine, and the protection of intellectual property rights after China's accession...

Examples

Embodiment 6

[0025] The quality control and evaluation method of embodiment Liuwei Dihuang Wan

[0026] 1. Screening of animal models of kidney yin deficiency

[0027] Liuwei Dihuang Wan is composed of Rehmannia glutinosa, Cornus officinalis, Chinese yam, Alisma, Moutan cortex and Poria cocos. It is a classic prescription for nourishing yin and tonifying kidney. Therefore, we must first establish and optimize an animal model of kidney yin deficiency that is more in line with the theory of traditional Chinese medicine. At present, the most commonly used animal models of kidney yin deficiency include thyroxine-type kidney-yin deficiency model, adrenal cortex hormone-type kidney-yin deficiency model, and heat-induced traditional Chinese medicine-type kidney-yin deficiency model.

[0028] Successful disease treatment must partially normalize defects in metabolic networks without interfering with the regulation of other metabolic pathways essential for maintaining health. After thousands of y...