A sample port of a cell culture system
A cell culture and sample technology, applied in the field of sample ports of cell culture systems, which can solve problems such as delays
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Embodiment 1
[0104] The construction of embodiment 1 medium-sized bioreactor platform
[0105] A prototype mesoscale bioreactor platform consisting of two layers of substrate material was designed using the 2D drawing software Auto-CAD LT (Autodesk, San Rafael, CA, USA).
[0106] The bioreactor platform is shown in Figure 7 and includes two storage chambers with a depth of 6 mm and diameters of 14 mm and 12 mm, 12 cultivation chambers with a diameter of 2.5 mm and a depth of 1.5 mm, and another chamber with a diameter of 7.9 mm and a depth of 6 mm room. The chambers are created by injection molding in an upper substrate material of black poly(methacrylate) (PMMA); the overall dimensions of the substrate are 74 x 7.4mm 2 . At the bottom of the sheet, channels (approximately 500 μm in diameter) were created using a Synrad Fenix Marker CO2-1aser (Synrad Inc., Mukilteo, WA, USA). The resulting substrate was then laser welded to a transparent PMMA substrate of the same size. The transpare...
Embodiment 2
[0110] The construction of embodiment 2 cell culture system
[0111]Two aluminum blocks are machined to support the appropriate size closure (approximately 10 x 7 x 3cm 3 The mesoscale bioreactor platform of Example 1 between two blocks of size).
[0112] The upper aluminum block was machined to just accommodate the bioreactor platform, and a hole (1 mm in diameter) was drilled in its upper block corresponding to the outlet of the medium-sized bioreactor platform. The opening of the hole was enlarged to accommodate an O-ring (inner diameter (ID) 1 mm), and the outlet hole was fitted with a 0.5 mm ID Teflon tube, which was connected to a micro-sized pH-electrode in a chamber in the lower surface of the upper aluminum block. Connect to define the sample port. The sample port is further connected to a 2mL syringe pump. The pH-electrode was connected to a sensorboard, which was further connected to a computer running LabView (version 8, National Instruments, Austin, Texas, USA)...
Embodiment 3
[0116] The construction of embodiment 3 cell culture system
[0117] In another design of the cell culture system, the lower aluminum block further includes a laminar airflow supply. This consists of a tube with a horizontal slit (1 mm high and 30 mm wide) located at the end corresponding to the platform of the bioreactor with the culture chamber (as shown in Figure 7) to introduce a width above the culture chamber that is in relation to the chamber width Similar horizontal laminar airflow. The tube has an entry point (on the outer surface of the upper aluminum block) for connection to an air supply, e.g. containing 5% CO 2 air.
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