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Method for fast propagating hydrilla varticillata through tissue culture

A technology of Hydra verticillium and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low multiplication coefficient and single culture ingredient base, reduce pollution, increase survival rate, and improve sterilization methods Effect

Inactive Publication Date: 2011-07-20
江苏九久环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is only one report on Hydrilla verticillium, and the selected culture ingredient base is relatively simple, and the obtained multiplication coefficient is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] Example one: a method for rapid propagation of Hydra verticillium tissue culture, comprising the following steps:

[0036](1) Collect the stem section of Hydrilla verticillium from the lake in the field in spring, wash it in tap water for 2 hours, remove impurities, put it into sterile water for 20 minutes, rinse it with sterile water for 3 times, and set it aside;

[0037] (2) Detoxification: Disinfect the Hydrilla verticillium stem section with a concentration of 70% alcohol for 4 seconds, rinse it with sterile water for 4 times, and put it into 0.1% mercuric chloride for sterilization After 3 minutes, rinse with sterile water 4 times, place on sterile paper to dry, and cut into 1cm;

[0038] (3) Induction culture: inoculate the cut H. verticillium stems into the induction medium for induction culture for 25 days, and induce and cultivate adventitious buds of H. verticillium; the temperature of the cultivation room is 25±2°C, the light intensity is 1200Lux, and the li...

example 2

[0042] Example two: a method for rapid propagation of Hydra verticillium tissue culture, comprising the following steps:

[0043] (1) Collect the stem section of Hydrilla verticillium from the lake in the field in spring, wash it in tap water for 4 hours, remove impurities, put it into sterile water for 30 minutes, rinse it with sterile water for 5 times, and set it aside;

[0044] (2) Detoxification: Disinfect the Hydra verticillium stem section with a concentration of 75% alcohol for 8 seconds, rinse it with sterile water for 6 times, and put it into 0.15% mercuric chloride for sterilization After 5 minutes, rinse with sterile water 6 times, place on sterile paper to dry, and cut into 2cm;

[0045] (3) Induction culture: inoculate the cut H. verticillium stems into the induction medium for induction culture for 30 days, and induce and cultivate adventitious buds of H. verticillium; the temperature of the culture room is 25±2°C, the light intensity is 1600Lux, and the light c...

example 3

[0049] Example three: a method for rapid propagation of Hydra verticillium tissue culture, comprising the following steps:

[0050] (1) Collect the stem section of Hydrilla verticillium from the lake in the field in spring, wash it in tap water for 3 hours, remove impurities, put it into sterile water for 25 minutes, rinse it with sterile water for 4 times, and set it aside;

[0051] (2) Detoxification: Disinfect the Hydra verticillium stem section with a concentration of 75% alcohol for 6 seconds, rinse it with sterile water for 5 times, and put it into 0.15% mercuric chloride for sterilization After 4 minutes, rinse with sterile water 5 times, place on sterile paper to dry, and cut into 1-2 cm;

[0052] (3) Induction culture: inoculate the cut H. verticillium stems into the induction medium for induction culture for 25 days, and induce and cultivate adventitious buds of H. verticillium; the temperature of the culture room is 25±2°C, the light intensity is 1500Lux, and the li...

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Abstract

The invention relates to a method for fast propagating hydrilla varticillata through tissue culture, comprising the following steps of: (1) collecting stem segments of the hydrilla varticillata; (2) detoxifying: detoxifying the stem segments of the hydrilla varticillata; (3) performing induced culture: inoculating the stem segments of the hydrilla varticillata into an induction culture medium for the induced culture so as to obtain adventitious buds of the hydrilla varticillata; (4) performing propagation culture: inoculating the adventitious buds of the hydrilla varticillata onto a propagation culture medium for the propagation culture so as to further culture a large quantity of the adventitious buds; (5) performing rooting culture: inoculating the adventitious buds obtained through the propagation culture onto a rooting culture medium for the rooting culture; and (6) opening a culture bottle of the hydrilla varticillata with root hairs, which is subjected to the rooting culture, placing under an indoor natural condition for seedling domestication, taking out tissue culture seedlings inside the culture bottle, cleaning, and transplanting into a pond for culture. The method provided by the invention meets the demands of ecological water body restoration and purification, fishery culture, organic fertilizer composting, and the like and can be used for sustainably and massively supplying the tissue culture seedlings of the hydrilla varticillata all the year round.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant tissue culture, in particular to a method for rapid propagation of tissue culture of Hydrilla verticillium, belonging to the field of biotechnology. Background technique [0002] Hydrilla verticillata is a perennial submerged herbaceous plant of the Hydrocharitaceae family. It generally grows in various waters in my country. It has strong pollution resistance and strong adaptability to eutrophication of water bodies. It can also grow in heavily polluted water bodies and can purify water bodies. In addition, this plant is generally planted in fish ponds to provide bait for shrimp, fish, poultry, etc., and this aquatic plant is planted on a large scale and in a large area. Plants provide organic matter for agricultural composting. [0003] At present, there is a great demand for H. verticillium seedlings in the market, and there are few studies on H. verticillium, which only focus on water p...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王远
Owner 江苏九久环境科技有限公司
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