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Antagonistic peptide of anti-opioid peptide and application thereof

A technology for anti-opioid peptides and antagonistic peptides, which is applied in the field of anti-opioid peptides and the application fields of the antagonistic peptides, and can solve the problems of acupuncture tolerance and the limited effective rate of acupuncture analgesia, etc.

Inactive Publication Date: 2011-07-20
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Professor Han Jisheng used an acupuncture treatment device to treat withdrawal symptoms, trying to stimulate the synthesis of endogenous opioids in the body through specific frequency electrical pulse stimulation to relieve symptoms, but its biggest defect is that the effectiveness of acupuncture analgesia in the population is limited and continues Acupuncture also has tolerance issues

Method used

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  • Antagonistic peptide of anti-opioid peptide and application thereof
  • Antagonistic peptide of anti-opioid peptide and application thereof
  • Antagonistic peptide of anti-opioid peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1. Acquisition of anti-opioid peptide 99A and its function verification

[0086] 1. The acquisition of anti-opioid peptide 99A in pig brain and its dose-dependent effect detection

[0087] 1. The discovery of anti-opioid peptides

[0088] In the isolated mouse vas deferens specimen, a square wave pulse (0.1MS wave width, 1 time / second) stimulation can cause contraction (refer to the literature for specific methods: J. Hughes et al (1975) Effect of morphine on adrenergic transmission in the Mouse Vas deferens.Assessment of agonist and antagonistpotencies of Narcotic analgesics 53,371-381), and then add morphine hydrochloride (3.2nM) or dog brain extract (AOS, 200μg / mL) (for dog brain extract) to the perfusate. The concentrated solution was filtered through Sephadex G-50 gel (purchased from Pharmacia) and carboxymethyl-cellulose (CM-C purchased from H. Reeve-Angel & Co. Ltd.) cation exchange chromatography to obtain a sample) , To observe the effect of electrical stimu...

Embodiment 2

[0118] Example 2. Obtaining of anti-opioid peptide 99A antagonistic peptide and its activity detection

[0119] 1. Obtainment of antagonistic peptides of 99A-16, 99A-19 and DBI-16

[0120] According to the principle of interaction between protein molecules, the reference (JRHeal et al Specific interactions between sense and complementary peptides: The Basis for the proteomic code ChemBIOChem 2002, 3, 114-131) designed 99A-16, 99A-19 and DBI -16 three short peptide antagonistic peptides, among them, the 99A-16 antagonistic peptide is named CP-99A-16+5, which has the amino acid residue sequence of SEQ ID NO: 5 and consists of 21 amino acid residues. The coding sequence has the nucleotide sequence of SEQ ID NO: 8 in the sequence list and consists of 63 bases; the 99A-19 antagonistic peptide is named CP-99A-19, which has the amino acid residue sequence of SEQ ID NO: 6 , Consists of 19 amino acid residues, and its coding sequence has the nucleotide sequence of SEQ ID NO: 9 in the seque...

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Abstract

The invention discloses anti-opioid peptide, and antagonistic peptide and application thereof. The anti-opioid peptide has one of the following amino acid residue sequences: 1) SEQ ID NO:1 in a sequence table; and 2) the amino acid residue sequence shown as the SEQ ID NO:1 in the sequence table is replaced and deleted by 1 to 10 amino acid residues or added with polypeptide with the effect of resisting morphine damages. The antagonistic peptide has one of the following amino acid residue sequences: 1) SEQ ID NO:5-7 in a sequence table; and 2) the amino acid residue sequence shown as the SEQ ID NO:5-7 in the sequence table is replaced and deleted by 1 to 10 amino acid residues or added with polypeptide with the effects of enhancing morphine analgesia, reversing morphine tolerance and / or weakening or eliminating abstinence syndromes. The anti-opioid peptide and an active segment and the antagonistic peptide thereof exert an important effect in the field of preparation of medicinal and drug treatment medicaments and have wide application prospects.

Description

[0001] This application is a divisional application with the application number 200710100384.4, the application date being June 11, 2007, and the invention-creation titled "Anti-opioid peptide and its antagonist peptide and application of the antagonist peptide". Technical field [0002] The present invention relates to peptides and their encoding genes and applications, and in particular to an anti-opioid peptide and its antagonist peptide with antagonistic morphine anti-damage effect, and the antagonist peptide is prepared to enhance morphine analgesia, reverse morphine tolerance and / or The application of drugs that reduce or eliminate the effects of withdrawal symptoms. Background technique [0003] As we all know, the rampant drug trafficking and drug abuse activities worldwide have become a cancer that endangers society, seriously disrupting orderly economic construction and social stability. In addition, intravenous drug use has also caused an increase in the infection rate o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/11C12N15/63C12N5/10A61K38/10A61P25/36A61P25/04
Inventor 陈玉珍吴才宏曲红
Owner PEKING UNIV
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