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Composition and use thereof

A composition and peroxidase technology, which are applied to the composition for measuring peroxide content and the application field of measuring hydrogen peroxide content, can solve the problems of insignificant color change gradient in detection results, inaccurate detection results and the like, and achieve The effect of stable color development and expansion of the detection concentration range

Active Publication Date: 2011-07-20
SHANGHAI ADICON CLINICAL LAB LNC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the content of the detected substance is high, for example, when the concentration of glucose in the sample is greater than 3g / L, the color gradient of the detection results of samples with different glucose concentrations is not obvious, making the detection results inaccurate

Method used

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  • Composition and use thereof
  • Composition and use thereof
  • Composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The composition without chromogenic agent stabilizer II is used to detect the content of glucose in urine

[0038]Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse it in solution II and bake in a 70°C oven for 15 minutes.

[0039] Solution I

[0040] drug

Dosage per 1000ml solution

purified water

1000ml

Citrate Buffered Salt

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0041] Solution II

[0042] drug

Dosage per 1000ml solution

acetone

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenyl semicarbazide

0.4-1.2g

[0043] Cut the above test paper into 5mm×5mm squares and paste them on a plastic card, immerse them in the standard solution with glucose content of 0.5g / L, 1.0g / L, 3.0g / L and 10g / L respectively, take out the test paper and absorb it. The excess solution was removed, and...

Embodiment 2

[0047] Embodiment 2 The composition containing 4-phenyl semicarbazide is used to detect the content of glucose in urine

[0048] Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse it in solution II, take it out and bake in a 70°C oven for 15 minutes.

[0049] Solution I

[0050] drug

Dosage per 1000ml solution

purified water

1000ml

Citrate Buffered Salt

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0051] Solution II

[0052] drug

Dosage per 1000ml solution

anhydrous ethanol

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenyl semicarbazide

0.4g

4-Phenyl semicarbazide

0.1～2g

[0053] Cut the above test paper into 5mm×5mm squares and paste them on a plastic card, immerse them in the standard products with glucose content of 0.5g / L, 1.0g / L, 3.0g / L and 10g / L respectively, take out the test...

Embodiment 3

[0057] The composition that embodiment 3 contains bisphenol A or gallic acid detects the content of glucose in urine

[0058] Immerse Whatman 3MM test paper in solution I, bake in a 70°C oven for 25 minutes, then immerse it in solution II, take it out and bake in a 70°C oven for 15 minutes.

[0059] Solution I

[0060] drug

Dosage per 1000ml solution

purified water

1000ml

Citrate Buffered Salt

1mol, pH5.5

glucose oxidase

120ku

Horseradish peroxidase

50ku

[0061] Solution II

[0062] drug

Dosage per 1000ml solution

anhydrous ethanol

1000ml

3,3',5,5'-Tetramethylbenzidine

3g

1-Phenyl semicarbazide

0.4g

Bisphenol A or gallic acid

0.8g

[0063] Cut the above test paper into 5mm×5mm squares and paste them on a plastic card, immerse them in the standard products with glucose content of 0.5g / L, 1.0g / L, 3.0g / L and 10g / L respectively, take out the test ...

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PUM

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Abstract

The invention provides a composition for measuring peroxide content in solution or the content of a peroxide generated by reaction. The composition comprises a peroxidase, a buffer salt, a color-developing agent and two color stabilizers, wherein one color stabilizer has a higher reducing capacity than the color-developing agent, and the other color stabilizer has a reducing capacity which is between that of the color-developing agent and that of the first color stabilizer. The composition enlarges the test range of the tested materials and makes color-developing effect stable.

Description

technical field [0001] The present invention relates to a composition and its application, in particular to a composition for measuring peroxide content and its application for measuring hydrogen peroxide content. Background technique [0002] The dry chemical test strip detection method can obtain various chemical or biological information of the tested sample in a very short time with a small amount of sample. As a screening test, dry chemical detection method is widely used in medical testing, food safety testing and other judgments. For example, dry chemical test strips are used to detect glucose and red blood cells in urine samples, to detect alanine aminotransferase in blood, and to detect pesticide residues in vegetables. [0003] The dry chemical test paper detection method includes two detection steps: "dipping" and "reading". According to different test items, the reaction reagents are pre-dried and fixed on the membrane carrier of dry chemical test paper. When ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
Inventor 李和平商涛廖青青
Owner SHANGHAI ADICON CLINICAL LAB LNC
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