Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles

A technology of biological macromolecules and gold nanoparticles, which is applied in the field of visual detection of biological macromolecules, can solve the problems of limited color change intensity or types of discoloration, affecting colorimetric results, false positive/negative, etc., and achieves a wide range of detection concentrations. , high accuracy and high specificity

Pending Publication Date: 2021-04-16
CENT SOUTH UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the current colorimetric sensors based on gold nanoparticles: first, changes in the external environment (temperature, pH, salt, and charged molecules) may lead to the aggregation of nanoparticles, and this unresponsive aggregation will lead to false positives / negatives. results, thereby affecting the results of the colorimetric
Secondly, some molecules identified on nanoparticles may need to be cumbersomely modified, which will lead to a very long detection process
Third, in some colorimetric experiments, the color change intensity or types of gold nano-solutions are limited, making colorimetric identification with the naked eye difficult (Ma X, He S, Qiu B, et al. Noble Metal Nanoparticle-Based Multicolor Immunoassays:An Approach toward Visual Quantification of theAnalytes with the Naked Eye[J].Acs Sensors,2019.)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles
  • Method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles
  • Method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The specific process of synthesizing nano-gold is as follows: First, add 100mL 0.01% (w / v) chloroauric acid (HAuCl 4 ) aqueous solution, and keep stirring, when the solution is heated to boiling, quickly add 2mL of 2% (w / v) sodium citrate, at this time there is an obvious color change, from colorless to dark blue to purple and finally wine red, At this time, continue to heat for 15 minutes, keep the reflux state and keep stirring during this process, remove the three-necked flask from the oil bath, place it on the stirrer and continue stirring, cool to room temperature, and transfer the synthesized nano-gold solution to a clean reagent in the bottle.

[0038] The characterization of nano-gold is mainly through three detection methods: firstly, the synthesized nano-gold is detected by ultraviolet spectrophotometer, and an obvious absorption peak appears at 518nm. According to the literature, the UV absorption coefficient of 13nm nanoparticles at 518 nm is 2.78×108M -1 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles, which comprises the following steps: inoculating a nanogold solution and a target biomacromolecule aptamer together to obtain an aptamer@AuNPs compound solution; respectively inoculating an aptamer@AuNPs compound solution with a series of standard target biomacromolecule solutions with different concentrations, adding a NaCl solution, controlling the pH value of the solution system, and establishing a concentration and color comparison card according to the relationship between the concentration and color development of the standard target biomacromolecule solutions; and after the standard target biomacromolecule solution is replaced with the standard target biomacromolecule solution to be detected for reaction, determining the concentration range of the target biomacromolecule solution to be detected according to the color displayed by the NaCl solution in the concentration and color comparison card. The method has the characteristics of high specificity, high sensitivity, wide detection range and the like; and visual detection of biomacromolecules can be realized.

Description

technical field [0001] The invention relates to a visual detection method of biological macromolecules, in particular to a method for visually distinguishing the biomacromolecules by using nucleic acid aptamers as recognition molecules and unmodified gold nanoparticles as signal probes. The concentration range of biological macromolecules belongs to the field of biological sensing. Background technique [0002] In recent years, with the rapid development of biosensors, detection methods for biomacromolecules (such as insulin, protein, etc.) have also become diverse, mainly including surface plasmon resonance, electrochemiluminescence, fluorescence, and electrochemical biosensing methods. Although these detection methods have high sensitivity and high selectivity, they are time-consuming, expensive, and require specific instruments and equipment, which will seriously affect their practical application. Therefore, it is of great significance to establish a simple, specific, s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 邓春艳谢云燕许游游
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com