Method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles

Abstract

The invention discloses a method for visually and specifically detecting biomacromolecules based on effective assembly of aptamers and gold nanoparticles, which comprises the following steps: inoculating a nanogold solution and a target biomacromolecule aptamer together to obtain an aptamer@AuNPs compound solution; respectively inoculating an aptamer@AuNPs compound solution with a series of standard target biomacromolecule solutions with different concentrations, adding a NaCl solution, controlling the pH value of the solution system, and establishing a concentration and color comparison card according to the relationship between the concentration and color development of the standard target biomacromolecule solutions; and after the standard target biomacromolecule solution is replaced with the standard target biomacromolecule solution to be detected for reaction, determining the concentration range of the target biomacromolecule solution to be detected according to the color displayed by the NaCl solution in the concentration and color comparison card. The method has the characteristics of high specificity, high sensitivity, wide detection range and the like; and visual detection of biomacromolecules can be realized.

Description

technical field [0001] The invention relates to a visual detection method of biological macromolecules, in particular to a method for visually distinguishing the biomacromolecules by using nucleic acid aptamers as recognition molecules and unmodified gold nanoparticles as signal probes. The concentration range of biological macromolecules belongs to the field of biological sensing. Background technique [0002] In recent years, with the rapid development of biosensors, detection methods for biomacromolecules (such as insulin, protein, etc.) have also become diverse, mainly including surface plasmon resonance, electrochemiluminescence, fluorescence, and electrochemical biosensing methods. Although these detection methods have high sensitivity and high selectivity, they are time-consuming, expensive, and require specific instruments and equipment, which will seriously affect their practical application. Therefore, it is of great significance to establish a simple, specific, s...

AI Technical Summary

Problems solved by technology

However, there are still many problems in the current colorimetric sensors based on gold nanoparticles: first, changes in the external environment (temperature, pH, salt, and charged molecules) may lead to the aggregation of nanoparticles, and this unresponsive aggregation will lead to false positives / negatives. results, thereby affecting the results of the colorimetric

Secondly, some molecules identified on nanoparticles may need to be cumbersomely modified, which will lead to a very long detection process

Third, in some colorimetric experiments, the color change intensity or types of gold nano-solutions are limited, making colorimetric identification with the naked eye difficult (Ma X, He S, Qiu B, et al. Noble Metal Nanoparticle-Based Multicolor Immunoassays:An Approach toward Visual Quantification of theAnalytes with the Naked Eye[J].Acs Sensors,2019.)

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