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Identification and evaluation method of sterilization effect of disinfectant

A technology of sterilization effect and evaluation method, which is applied in the direction of biochemical equipment and methods, microorganism measurement/inspection, etc., can solve the problems of short detection cycle and long time, and achieve the effect of saving time and wide detection concentration range

Pending Publication Date: 2020-02-21
SHIJIAZHUANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method can only dilute the disinfectant according to a certain concentration gradient, and the detection process takes a long time. Therefore, there is an urgent need for a detection method that is suitable for various concentrations of disinfectants and has a short detection cycle.

Method used

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  • Identification and evaluation method of sterilization effect of disinfectant
  • Identification and evaluation method of sterilization effect of disinfectant
  • Identification and evaluation method of sterilization effect of disinfectant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Arrange 8 sterilized test tubes on the test tube rack, and mark the test tube number; (2) Add 0.9% normal saline after sterilization to each test tube, and add 9 mL to test tubes 1-5, 6-8 Add 10mL to No. test tube, put 8 test tubes into a water bath at 20±2°C; (3) Add 1mL bacterial solution to No. 1 test tube and mix well, transfer 1mL into No. Take three 1mL samples and transfer them into test tube No. 3, test tube 4, and test tube No. 5 respectively. After mixing the solutions in test tubes 3-5, pour the solutions in test tubes 6-8 into tubes 3-5 in turn, and mix well. Then pipette 10mL of the solution from tubes 3-5 into test tubes 6-8. (4) Take 30 μl of normal saline from No. 3 test tube, then take 30 μl of 1000 ppm PHMG disinfectant (that is, the disinfectant concentration is 3 ppm) and mix it in No. 3 test tube, take out 50 μl of normal saline from No. 4 test tube, and then remove the disinfectant Mix 50 μl in test tube No. 4, take 100 μl of normal saline fro...

Embodiment 2

[0035](1) Arrange 6 sterilized test tubes on the test tube rack, and mark the test tube number; (2) Add 0.9% normal saline after sterilization to each test tube, among which 9 mL is added to test tubes 1-4, 5-6 Add 10mL to the No. test tube, put the 6 test tubes in a water bath at 20±2℃; (3) Add 1mL of bacterial solution to the first test tube and mix well, transfer 1mL into the second test tube, mix again, Take three 1mL tubes and transfer them into the 3rd test tube and the 4th test tube respectively. After mixing the 3-4 test tubes respectively, pour the solutions of the 6-8 test tubes into the 3-5 tubes in turn, mix well, and then respectively from the 3 - Tube No. 5 Pipette 10 mL of the solution into No. 6-8 test tubes. (4) Take out 30 μl of normal saline from No. 3 test tube, then take 30 μl of nano-silver ion antibacterial agent of 10000ppm (that is, the concentration of antibacterial agent is 30ppm) and mix in No. 3 test tube, take out 60 μl of normal saline from No. 4...

Embodiment 3

[0038] (1) Arrange 8 sterilized test tubes on the test tube rack, and mark the test tube number; (2) Add 0.9% normal saline after sterilization to each test tube, and add 9 mL to test tubes 1-5, 6-8 Add 10mL to the No. test tube, and put 8 test tubes into a water bath at 20±2°C; (3) Add 1mL of the bacterial solution to the first test tube and mix well, then transfer 1mL into the second test tube, mix well again, and transfer from the second test tube Take three 1mL tubes and transfer them into the 3rd test tube, 4th test tube, and 5th test tube respectively. After mixing the test tubes 3-5, pour the solution from the 6-8 test tubes into the 3-5 tubes in turn, and mix well. , and then pipette 10mL of the solution from tubes 3-5 into test tubes 6-8. (4) Take out 50 μl of normal saline from No. 3 test tube, then take 50 μl of chitosan disinfectant of 10000 ppm (that is, the concentration of disinfectant is 50 ppm) and mix it in No. 3 test tube, take out 100 μl of normal saline fr...

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Abstract

The invention discloses an identification and evaluation method of sterilization effect of a disinfectant, and belongs to the technical field of measurement or detection of microorganisms. The methodcomprises the following steps: (1) marking 8 testing tubes; (2) adding physiological saline: No. 1- No. 5: 9 mL of physiological saline and No. 6- No. 8: 10 mL of physiological saline, in a 20 + / - 2 DEG C water bath; (3) adding 1 mL of bacterial solution into the No. 1 testing tube, taking 1 mL of solution from the No. 1 testing tube and transferring into the No. 2 testing tube, taking 1 mL of solution from the No. 2 testing tube and transferring into the No. 3-No. 5 testing tubes, and then the solution in the No. 6-No. 8 testing tubes is poured into the No. 3-No. 5 testing tubes, respectivelyand taking 10 mL of solution from the No. 3-No. 5 testing tubes and transferring into the No. 6-No. 8 testing tubes, respectively; (4) adding a disinfectant into the No. 3-No. 7 testing tubes as experimental samples; and the No. 8 testing tube without adding any disinfectant as control sample; (5) conducting qualitative and quantitative culture; (6) observing the results and calculating the bacteriostatic rate. The detection method of the invention can detect any concentration of disinfectant, can simultaneously detect the bacteriostatic effect of two or more batches of disinfectant, has a wide detection concentration range, and can save time.

Description

technical field [0001] The invention relates to the technical field of measurement or detection of microorganisms. Background technique [0002] With the improvement of living standards, people's requirements for hygiene are also increasing. With the production of various disinfectants, people's requirements for their antibacterial effects are getting higher and higher. The currently implemented detection method is GB15981-1995. After diluting the disinfectant to a series of concentration gradients with distilled water, add the diluted bacterial suspension and mix evenly for a period of time, then take a small amount of neutralizing agent and neutralize for 10 minutes. Take out a small amount and add it to nutrient broth, and observe whether it is turbid to judge its antibacterial effect. Although the detection method requires simple equipment, obvious phenomena, and is easy to observe, it can accurately evaluate the killing effect of disinfectants on microorganisms. Howe...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 王娟张文英南紫萱梁芳慧岳琳张伟亮贾鹏飞葛凤燕张星辰刘占旗邢云立章柏宁
Owner SHIJIAZHUANG UNIVERSITY
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