Preparation method of plantago seed polysaccharide extractive and applications thereof

A technology of psyllium polysaccharides and extracts, applied in medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc.

Active Publication Date: 2011-08-03
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although there are invention reports about the anti-oxidation and free radical scavenging of psyllium polysaccharides, they are quite different from this patent in the preparation and refining proces...

Method used

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  • Preparation method of plantago seed polysaccharide extractive and applications thereof
  • Preparation method of plantago seed polysaccharide extractive and applications thereof
  • Preparation method of plantago seed polysaccharide extractive and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take about 2 kg of plantago medicinal material, reflux extraction with 90% ethanol for 3 times, 2 hours each time, filter, dry the filter residue, add 12 times the amount of distilled water to the residue, reflux extraction 3 times, 2 hours each time, filter, combine the filtrate, and decompress Recover the solvent to 1 times the volume of the medicinal material, adjust the alcohol content to 80% with 95% ethanol, let it stand overnight, centrifuge at 2000rpm / min, and wash the precipitate with 4 times the amount of absolute ethanol and acetone in turn to obtain the total polysaccharide. The total polysaccharide was dissolved in water, and the supernatant was dialyzed with a dialysis bag with a molecular weight of 3500 for 48 hours. The dialysate was concentrated under reduced pressure to 1 volume of the medicinal material, and 95% ethanol was added to adjust the alcohol content to 80%. It was allowed to stand overnight and centrifuged at 2000rpm / min. The precipitate was ...

Embodiment 2

[0025] Take about 3 kg of plantago medicinal material, reflux extraction with 80% alcohol for 2 times, 3 hours each time, filter, dry the filter residue, add 10 times the amount of distilled water to the residue, reflux extraction 2 times, 3 hours each time, filter, combine the filtrate, and decompress Recover the solvent to 1 times the volume of the medicinal material, adjust the alcohol content to 85% with 95% ethanol, let stand overnight, centrifuge at 2000rpm / min, and wash the precipitate with 4 times the amount of absolute ethanol and acetone in turn to obtain the total polysaccharide. Take the total polysaccharide and dissolve it in water, dialyze the supernatant with a dialysis bag with a molecular weight of 7000 for 48 hours, concentrate the dialysate to 1 volume of the medicinal material under reduced pressure, add 95% ethanol to adjust the alcohol content to 85%, let stand overnight, centrifuge at 2000rpm / min, and precipitate Wash with 4 times the weight of absolute e...

Embodiment 3

[0026] Embodiment 3 is made transfusion

[0027] The total polysaccharides were further purified and separated by Sephacryl S300 and Sephadex G50 chromatography on agarose gel column: to obtain a polysaccharide extract with a purity of more than 98%, add an appropriate amount of solubilizer, grind, then add a small amount of water for injection to dilute, and mix well , and then add an appropriate amount of sodium chloride, dissolve and then add water for injection to the specified amount, filter, pot, and sterilize to obtain.

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Abstract

The invention relates to a preparation method of an immunosuppressive activity plantago seed extractive and applications thereof. The preparation method comprises the following steps of: degreasing raw materials and obtaining crude extractive through water extraction and alcohol precipitation; deproteinizing and depigmenting the crude extractie through dialysis and ion exchange chromatography; and removing micromolecular chemical compositions to prepare the plantago seed extractive with higher purity. A total polysaccharide content in the inventive immunosuppressive activity plantago seed extractive accounts more than 72% of the extractive by weight. Shown by a great amount of pharmacological experiments, the plantago seed extractive has immunosuppressive activity, can be used for preparing immune inhibitors in various preparation forms or used as a raw material medicine for preparing other immunosuppressive medicines and used for medical purposes in treating diseases related to immune inflammatory injuries, such as nephritis, cystitis, prostatitis, urethritis, edema and eye diseases, such as uveitis, interstitial keratitis, corneal transplantation rejection, and the like.

Description

technical field [0001] The present invention relates to a preparation method of polysaccharide extract of Semen psyllium and the polysaccharide extract of Semen psyllium is used for treating diseases related to immune inflammatory damage: nephritis, cystitis, prostatitis, urethritis through immunosuppressive activity , edema, and eye diseases such as uveitis, keratitis, and corneal transplantation rejection. Background technique [0002] Semen psyllium is the dry mature seed of Plantago asiatica L. or Plantago depressa Willd. Nature and flavor are sweet and cold, enter the kidney and bladder meridians, have diuretic, heat-clearing, eyesight-improving, and expectorant effects, and are used for urinary obstruction, stranguria with leukorrhagia, hematuria, heat-damp diarrhea, cough with phlegm, arthralgia due to dampness, It is widely used clinically for diseases such as erythematous nebula. Now chemical studies generally believe that plantain seed contains a lot of mucus, ps...

Claims

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Application Information

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IPC IPC(8): A61K36/68A61P37/06A61P7/10A61P13/02A61P13/08A61P13/10A61P13/12A61P27/02A61K131/00
Inventor 匡海学王秋红杨炳友夏永刚舒尊鹏薛娟
Owner 匡海学
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