Methylated marker IL-2RG in systemic lupus erythematosus (SLE) whole blood genome and application thereof

A technology of IL-2RG and lupus erythematosus, which is applied to the methylation marker IL-2RG in the whole blood genome of systemic lupus erythematosus and its application field, can solve the problems of low patient compliance, time-consuming and labor-intensive, etc. Time to diagnosis, reduced diagnostic cost, disease activity, and precise effects on efficacy and prognosis assessment

Inactive Publication Date: 2011-08-03
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The whole experiment process is time-consuming and labor-intensive, and 60ml of peripheral venous blood is drawn, and the patient's compliance is often low

Method used

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  • Methylated marker IL-2RG in systemic lupus erythematosus (SLE) whole blood genome and application thereof
  • Methylated marker IL-2RG in systemic lupus erythematosus (SLE) whole blood genome and application thereof
  • Methylated marker IL-2RG in systemic lupus erythematosus (SLE) whole blood genome and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0036] 1 DNA Extraction from Peripheral Blood Tissue

[0037] 1. Steps to extract DNA from 1ml whole blood or body fluid:

[0038] (1) Lysis 1: Take 1ml of whole blood, plasma, serum, and lymphocytes into a 1.5ml centrifuge tube, and add 400ul of Lysis Solution 1. Flip up and down to disperse the sediment, rotate and pulsate for 15 seconds, then put it into a centrifuge for centrifugation at 8000rpm, 1min. Pour off the upper layer, and it can be seen that there is a bloody precipitate at the bottom of the centrifuge tube. Repeat this lysis step once, this time adding 1 ml of Lysis Buffer 1. Finally, suck up all the supernatant with a pipette and discard it, so that the blood-colored precipitate at the bottom of the centrifuge tube will no longer contain lysate 1.

[0039] (2) Lysis 2: Add 1ml of Lysis Solution 2 to the centrifuge tube above. Flip up and down to disperse the sediment, spin and pulsate for 15 seconds and put it in a centrifuge for centrifugation at 8000rpm f...

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Abstract

The invention discloses a methylated marker IL-2RG in a systemic lupus erythematosus (SLE) whole blood genome and application thereof. The sequence of the marker is shown as SEQ ID NO:1. The methylated marker IL-2RG in the SLE whole blood genome is applied to the preparation of a reagent for diagnosing SLE, in particular to a kit for diagnosing SLE. By detecting the methylation level of the methylated marker IL-2RG, a specific methylation locus with a great meaning and a remarkable difference is counted and taken as a basis for early diagnosis of SLE. By adopting the methylated marker, an accurate, cheap and quick clinical tool for early diagnosis of SLE, the disease activity, the curative effect and the prognostic evaluation is provided.

Description

technical field [0001] The invention relates to whole blood genome methylation biomarker IL-2RG for early diagnosis of systemic lupus erythematosus and application thereof. Background technique [0002] Systemic Lupus Erythematosus (SLE) is an autoimmune disease involving multiple organs and systems. As a typical autoimmune disease that seriously endangers human health, its etiology and pathogenesis are very complicated, and the medical field is still not very clear. At present, the diagnosis of SLE mainly relies on clinical manifestations and laboratory tests. Clinical manifestations include butterfly erythema on the cheeks, etc. Laboratory tests mainly include lupus band test and antinuclear antibody spectrum. However, when the diagnosis is made based on clinical manifestations and laboratory tests, patients often have multiple organ systems involved. Therefore, if the diagnosis of SLE can be raised to the genetic level and a rapid diagnostic tool can be found for clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陆前进马乐赵明谭怡忻廖洁月尹恒
Owner CENT SOUTH UNIV
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