Tissue culture method for flower buds of hemerocallis middendorfii 'prunus lannesiana wils'

Abstract

The invention discloses a tissue culture method for the flower buds of the hemerocallis middendorfii 'prunus lannesiana wils', which comprises the steps of: soaking the flower buds by cleanser essence, 75% alcohol and a 0.1% HgCl2 solution sequentially to obtain the sterile flower buds, wherein the sterilization effect is better, the follow-up culture pollution is less, and the death rate of the flower buds is lower; culturing in an inducing way by an inducing culture medium to form into the callus so as to obtain the callus flower buds; and culturing by a differential culture medium to form into the adventitious buds so as to obtain the tissue culture seedlings of the 'prunus lannesiana wils'. The method adopts the hierarchical culture of the callus buds and the adventitious buds, wherein the adventitious buds are faster in propagation and better in quality, one flower bud of the hemerocallis middendorfii 'prunus lannesiana wils' can propagate 20 thousands available root growing tissue culture seedlings, the adventitious buds can be propagated at a time every one moth, and the propagation coefficient is always kept at 4.2-5.2, so that the tissue culture method is short in culture period, and lays a foundation for the high-propagation rate factorial production of the hemerocallis middendorfii 'prunus lannesiana wils' on a large scale.

Description

technical field [0001] The invention relates to tissue culture technology of Hemerocallis grandiflorum, in particular to a tissue culture method for flower buds of Hemerocallis grandiflora "Yuyihuang". Background technique [0002] Hemerocallis middendorfii (Hemerocallis middendorfii) is a perennial herbaceous plant of the genus Hemerocallis in the family Liliaceae. It has gorgeous colors, leaves like bluegrass, flowers like lilies, and has a long flowering period. Hemerocallis grandiflora has a wide variety of flower colors, including light yellow, golden yellow, light yellow, light red, bright red, deep red and other colors, and there are more pink and red and yellow alternate colors. In recent years, my country has successively introduced new varieties of Hemerocallis grandiflora from abroad to meet the market demand. Among them, Hemerocallis grandiflora "Yuyihuang" is a newly introduced variety with a large flower shape and eye-catching orange-yellow color. It blooms fro...

AI Technical Summary

Problems solved by technology

However, the research on the tissue culture of "Yuyihuang" has not been reported. Therefore, exploring the tissue culture technology of "Yuyihuang" and realizing its large-scale reproduction will not only enrich the varieties of landscaping plants and meet the market demand, but also contribute to the development of other plants in the Liliaceae family. The study of tissue culture technology has important reference significance