Method for determining antiproteinase 3 antibody IgG (intravenous gamma globulin) and reagent device

An anti-protease and antibody technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high price, cumbersome and complicated operation process, and operation errors of automatic enzyme immunoassay analyzers, so that it is not easy to operate errors, operate Convenience and the effect of ensuring correctness

Inactive Publication Date: 2011-08-10
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) False positives may occur using this method
[0009] (2) When analyzing the results, non-specific recognition cannot be distinguished according to the size of the molecular weight
[0010] (3) The operation is relatively complicated and requires a more expensive fluorescence microscope, which is difficult to promote in many primary hospitals and is not suitable for laboratories with a large number of specimens
[0011] (4) The background in fluorescence measurement is high, and it is difficult to use fluorescence immunoassay for quantitative determination
[0012] (5) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient
[0017] (2) There can be as many as 11 kinds of reagents used for quantitative determination, and each detection reagent should be contained in a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to add to the microwell plate respectively. In the well, not only are there many types of reagent bottles, but also the operation of adding reagents is extremely c...

Method used

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  • Method for determining antiproteinase 3 antibody IgG (intravenous gamma globulin) and reagent device
  • Method for determining antiproteinase 3 antibody IgG (intravenous gamma globulin) and reagent device
  • Method for determining antiproteinase 3 antibody IgG (intravenous gamma globulin) and reagent device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Indirect enzyme-linked immunoassay method and kit and reagent device for detecting anti-protease 3 antibody IgG

[0076] The present invention is a set of simpler, more accurate and effective methods based on enzyme-linked immunoassay technology, and the basic principle adopted is indirect enzyme-linked immunoassay. The highly purified protease 3 antigen is adsorbed on the solid phase, and the specific antibody in the diluted human serum is combined with the antigen by incubation, the antibody that is not bound to the solid phase is removed by washing, and the anti-antibody labeled with horseradish peroxidase is added. Human Immunoglobulin Enzyme Conjugate, Incubation. Unbound enzyme conjugate is removed and enzyme chromogen substrate is added. The color produced is directly proportional to the concentration of specific antibody in the test sample. This method mainly realizes the immunodetection of anti-protease 3 antibody IgG through an analysis reagent dev...

Embodiment 2

[0079] Example 2 Production of Reagent Device or Kit - Indirect Method for Detection of Anti-Protease 3 Antibody IgG

[0080] Use hole 1 as the container for the sample to be tested, add liquid samples when in use, and take it for testing;

[0081] Use hole position 2 as an empty hole, seal it with a sealing film, and reserve it for testing;

[0082] Use hole 3 as the reagent container, add the sample dilution reagent and seal it with a sealing film for use during testing;

[0083] Use hole 4 as the reagent container, add the stop reagent and seal it with a sealing film for detection;

[0084] Use hole 5 as the reagent container, add horseradish peroxidase-labeled anti-human IgG antibody reagent and seal it with a sealing film for detection;

[0085]Use hole 6 as the reagent container, add the enzyme reaction substrate reagent and seal it with a sealing film for detection;

[0086] Well position 7 is used as coating well / reaction container / colorimetric well, which has been ...

Embodiment 3

[0090] Example 3 Realize the analysis operation flow of the sample anti-protease 3 antibody IgG detection by a fully automatic analyzer

[0091] The automatic analyzer includes an analysis device tray, which matches the shape of the analysis device, and there are 30 positions for the analysis device to be placed for detection and analysis. In addition, it also includes a modular integrated mechatronic structure, which can realize the automation of sample addition, dilution, incubation, washing and reading process. Each position is independently quantitatively analyzed, and more than 200 electronic sensors monitor the operation of the instrument to ensure the accuracy of the results. After the instrument is running, the analysis device tray will automatically rotate to different positions for the steps of adding samples, diluting, incubating, washing and reading.

[0092] (1) boot

[0093] After the instrument switch is turned on, the instrument will automatically perform a s...

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Abstract

The invention provides a method for realizing immunization detection of antiproteinase 3 antibody IgG (intravenous gamma globulin) based on the enzyme-linked immunoassay principle and a reagent device therefor; and an analytic method, the reagent device and an assorted reagent are independently, individually and disposably used for detecting the antiproteinase 3 antibody IgG based on enzyme-linked immunoassay. Various reagents needed by the enzyme-linked immunoassay for the antiproteinase 3 antibody IgG are contained in one analytic device; and by using the method, the related immunology detection can be conveniently carried out according to the using requirements of detection items so as to provide better basis for clinical application.

Description

technical field [0001] The application of the present invention relates to a method and a reagent device for measuring anti-protease 3 antibody IgG, which belong to the technical field of biological detection. Background technique [0002] Antineutrophil cytoplasmic antibodies (antineutrophil cytoplasmic antibodies. ANCA) is currently the most important serological detection indicator for the diagnosis of primary vasculitis, and proteinase 3 (PR3) is one of the main target antigens of cANCA , accounting for about 80% to 90% of C-ANCA. [0003] Wegener's granulomatosis (WG) is a special type of necrotizing granulomatous vasculitis. Its clinical complexity and difficulty in diagnosis and treatment have always been problems for rheumatologists. The diagnosis of WG is mainly based on clinical symptoms, and the clinical manifestations are complex and diverse, and vary from person to person. Antineutrophil cytoplasmic antibody (ANCA) is the most diagnostic autoantibody for WG. ...

Claims

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Application Information

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IPC IPC(8): G01N33/573
Inventor 何林潘荞肖灿伍坚
Owner SHENZHEN YHLO BIOTECH
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