Epulorhizarepens and application thereof to growth promotion of dendrobium loddigesii rolfe
A technology of mycorrhizal fungus and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of low reproduction coefficient, slow growth and low survival rate of Dendrobium candidum.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Embodiment 1: the acquisition of the nodular mycorrhizal fungus of the present invention
[0035] Wash the roots of Guizhou Chunlan, refer to the separation method of single mycelia (patent number: ZL200610051196.2), inoculate on PDA medium, purify, and produce nodular mycorrhizal fungi Epulorhiza repens The CG035 strain was stored at 4°C for later use.
Embodiment 2
[0036] Embodiment 2: the symbiotic culture of the CG035 bacterial strain gained in embodiment 1 and the tissue-cultured plantlet of Dendrobium nobile
[0037] The CG035 strain activated on the PDA medium was punched with a hole diameter of 5.0 mm to take a bacterial slice and inoculate it on the symbiotic medium (the volume ratio of vermiculite: sawdust: moss fresh was 1:1:1, at 121 ℃ for 1 hour, and 1 day later, the same method was used to sterilize again), cultured for 15 days, and the 3-4 leaf tissue cultured seedlings were inserted into the symbiotic medium under sterile conditions, and the temperature and light were cultivated under natural conditions.
Embodiment 3
[0038] Embodiment 3: the identification of nodular mycorrhizal fungus of the present invention
[0039]Identification of morphological characteristics: Refer to the identification method (Currah R.S. et al., 1997; Moore R. T., 1987) to detect the growth rate, the size of hyphae and moniliform cells, the number of nuclei, the presence or absence of hyphae septum, and whether there is perforation , shape etc. The results show that: the bacteria grows on the standard BDPDA medium, grows on the mycelia paste medium in the early stage, the mycelium is milky white, waxy, the colony edge is neat, the thickness is uniform, the colony edge invades the medium and grows, and the sparse white cobweb-like aerial mycelium is produced in the later stage , mycelium growth rate 0.088 mm / h, mycelium diameter 2.27-5.37 μm, cell length 13.05-98.65 μm, bead-shaped cells spherical, often forming long chains, size 6.17-18.25×7.47-16.35 μm, hyphae with septa, The nucleus is binucleate. The mycelial...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com