Epulorhizarepens and application thereof to growth promotion of dendrobium loddigesii rolfe

A technology of mycorrhizal fungus and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of low reproduction coefficient, slow growth and low survival rate of Dendrobium candidum.

Inactive Publication Date: 2011-08-17
GUIZHOU FRUIT INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of slow growth, low reproduction coefficient and low survival rate of the ex...

Method used

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  • Epulorhizarepens and application thereof to growth promotion of dendrobium loddigesii rolfe
  • Epulorhizarepens and application thereof to growth promotion of dendrobium loddigesii rolfe
  • Epulorhizarepens and application thereof to growth promotion of dendrobium loddigesii rolfe

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Embodiment 1

[0034] Embodiment 1: the acquisition of the nodular mycorrhizal fungus of the present invention

[0035] Wash the roots of Guizhou Chunlan, refer to the separation method of single mycelia (patent number: ZL200610051196.2), inoculate on PDA medium, purify, and produce nodular mycorrhizal fungi Epulorhiza repens The CG035 strain was stored at 4°C for later use.

Embodiment 2

[0036] Embodiment 2: the symbiotic culture of the CG035 bacterial strain gained in embodiment 1 and the tissue-cultured plantlet of Dendrobium nobile

[0037] The CG035 strain activated on the PDA medium was punched with a hole diameter of 5.0 mm to take a bacterial slice and inoculate it on the symbiotic medium (the volume ratio of vermiculite: sawdust: moss fresh was 1:1:1, at 121 ℃ for 1 hour, and 1 day later, the same method was used to sterilize again), cultured for 15 days, and the 3-4 leaf tissue cultured seedlings were inserted into the symbiotic medium under sterile conditions, and the temperature and light were cultivated under natural conditions.

Embodiment 3

[0038] Embodiment 3: the identification of nodular mycorrhizal fungus of the present invention

[0039]Identification of morphological characteristics: Refer to the identification method (Currah R.S. et al., 1997; Moore R. T., 1987) to detect the growth rate, the size of hyphae and moniliform cells, the number of nuclei, the presence or absence of hyphae septum, and whether there is perforation , shape etc. The results show that: the bacteria grows on the standard BDPDA medium, grows on the mycelia paste medium in the early stage, the mycelium is milky white, waxy, the colony edge is neat, the thickness is uniform, the colony edge invades the medium and grows, and the sparse white cobweb-like aerial mycelium is produced in the later stage , mycelium growth rate 0.088 mm / h, mycelium diameter 2.27-5.37 μm, cell length 13.05-98.65 μm, bead-shaped cells spherical, often forming long chains, size 6.17-18.25×7.47-16.35 μm, hyphae with septa, The nucleus is binucleate. The mycelial...

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Abstract

The invention discloses an epulorhizarepens strain CG035 and application thereof to growth promotion of dendrobium loddigesii rolfe. The strain CG035 has a preservation number of CCTCC NO.M2010370 and can be cultured with the dendrobium loddigesii rolfe in a symbiont way for promoting the growth of the dendrobium loddigesii rolfe.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a nodular mycorrhizal fungus and its application in promoting the growth of Dendrobium candidum. Background technique [0002] There is a close relationship between orchids and their mycorrhizal fungi. In the natural state, mycorrhizal fungi are required from the seeds of orchids to germinate into protocorms (rhizomes) to the first true leaf growth stage, and even some leafy seedlings Growth and development are completely or partially dependent on mycorrhizal fungi, which can provide N sources, C sources and minerals for Orchidaceae. Studies have shown that the same fungus can co-exist with a variety of orchids. Studies have reported that orchid mycorrhizal fungi secrete B vitamins and gibberellins, which have an impact on plant growth. [0003] Herba Dendrobii ( Dendrobium loddigesii Rolfe) is a perennial herb of the genus Dendrobium orchidaceae, an authentic medicinal...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/645
Inventor 桂阳朱国胜刘作易
Owner GUIZHOU FRUIT INST
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