Preparation method of human homologous tendon
A tendon and human technology, which is applied in the preparation of allogeneic tendon and the field of human allogeneic tendon preparation, can solve the problems of complex operation steps, large changes in the original structure and strength of tendon, and many drug residues, so as to maintain strength, Efficient killing effect and the effect of maintaining mechanical properties
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Embodiment 1
[0059] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:
[0060] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;
[0061] 2) Store the cleaned tendon material in a freezer at -60°C for 30 days;
[0062] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 30 minutes, and cut off the loose connective tissue around the tendon;
[0063] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 0.05:10:70, and let it stand for 20 minutes to inactivate the virus;
[0064] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 0.1 ppm;
[0065] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice ...
Embodiment 2
[0068] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:
[0069] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;
[0070] 2) Store the cleaned tendon material at -70°C for 60 days;
[0071] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 45 minutes, and cut off the loose connective tissue around the tendon;
[0072] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 1:15:80, and let it stand for 35 minutes to inactivate the virus;
[0073] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 3 ppm;
[0074] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;
[0075] 7...
Embodiment 3
[0077] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:
[0078] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;
[0079] 2) Store the cleaned tendon material at -80°C for 90 days;
[0080] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 60 minutes, and cut off the loose connective tissue around the tendon;
[0081] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 2:30:90, and let it stand for 50 minutes to inactivate the virus;
[0082] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 5 ppm;
[0083] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;
[0084] 7...
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