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Preparation method of human homologous tendon

A tendon and human technology, which is applied in the preparation of allogeneic tendon and the field of human allogeneic tendon preparation, can solve the problems of complex operation steps, large changes in the original structure and strength of tendon, and many drug residues, so as to maintain strength, Efficient killing effect and the effect of maintaining mechanical properties

Inactive Publication Date: 2011-08-31
山西奥瑞生物材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems of immune rejection and bacterial and viral infection in the current allogeneic tendon transplantation, complicated operation steps, many drug residues, and large changes in the original structure and strength of the tendon, and provide an immune rejection-free and Bacterial and viral infection, simple operation, no drug residue, no change in the original structure and strength of the human tendon preparation method

Method used

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  • Preparation method of human homologous tendon
  • Preparation method of human homologous tendon
  • Preparation method of human homologous tendon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0060] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0061] 2) Store the cleaned tendon material in a freezer at -60°C for 30 days;

[0062] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 30 minutes, and cut off the loose connective tissue around the tendon;

[0063] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 0.05:10:70, and let it stand for 20 minutes to inactivate the virus;

[0064] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 0.1 ppm;

[0065] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice ...

Embodiment 2

[0068] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0069] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0070] 2) Store the cleaned tendon material at -70°C for 60 days;

[0071] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 45 minutes, and cut off the loose connective tissue around the tendon;

[0072] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 1:15:80, and let it stand for 35 minutes to inactivate the virus;

[0073] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 3 ppm;

[0074] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;

[0075] 7...

Embodiment 3

[0077] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0078] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0079] 2) Store the cleaned tendon material at -80°C for 90 days;

[0080] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 60 minutes, and cut off the loose connective tissue around the tendon;

[0081] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 2:30:90, and let it stand for 50 minutes to inactivate the virus;

[0082] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 5 ppm;

[0083] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;

[0084] 7...

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Abstract

The invention relates to a preparation method of human homologous tendon, which belongs to the technical field of biomedical materials. The invention mainly solves the technical problems of immunological rejection, bacterial infection, virus infection, complicated operation steps, and the like in the traditional method for transplanting homologous tendon. The preparation method of human homologous tendon comprises the following steps of: (1) cleaning the human homologous tendon; (2) freezing the cleaned human homologous tendon for 30-90 days; (3) trimming loose connective tissues surrounding the tendon; (4) immersing the trimmed tendon material in a mixed solution of peroxyacetic acid, ethanol and water, standing still for inactivation of viruses; (5) cleaning the inactivated tendon with water until the content of the peroxyacetic acid in the cleaning solution reaches a certain value for the last time; (6) trimming the tendon again, dripping the water on the surface of the tendon, vacuumizing the trimmed tendon under an aseptic condition, and packing twice by common packaging; and (7) radiating the packed tendon by using Cobalt-60 for disinfection again. The invention has the advantages of no immunological rejection, no bacterial or virus infection, easiness for operation, no drug residue, and the like.

Description

technical field [0001] The invention relates to a preparation method of human allogeneic tendon, in particular to a preparation method which can be used for making allogeneic tendon, and belongs to the technical field of medical biomaterials. Background technique [0002] Tendon is a regularly arranged dense connective tissue composed of collagen, tenocytes and tendon matrix. Collagen is the main component of tendon, accounting for 70-90% of the dry weight of tendon, and plays a leading role in maintaining the strength of tendon. Tenocytes are a kind of fibroblasts with a special shape and a small number, which are the main carriers of antigens in allogeneic tendon transplantation. The tendon matrix provides the structural support for the collagen tissue, and its elasticity also helps reduce friction and shear forces acting on the tendon. Tendons connect skeletal muscle to muscle tissue, attach the muscle tissue to bone or fascia, transmit force to the skeletal system, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 李宝兴李幼忱周沫李宝明马绍英赵亚平陈学英张育敏张乃丽刘晓明
Owner 山西奥瑞生物材料有限公司
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