Plate-frame immobilized cell membrane bioreactor and application thereof
An immobilized cell and bioreactor technology, applied in anaerobic digestion treatment and other directions, can solve the problems of increased ethanol consumption, unfavorable engineering application, slow denitrification rate, etc., to reduce adverse effects, improve processing capacity, and reduce waste. Effect
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Embodiment 1
[0021] Example 1: Construction of a plate and frame type immobilized cell membrane bioreactor.
[0022] (1) Enrichment culture of denitrifying bacteria: add 2000mg / L potassium nitrate, 1500mg / L ethanol, 350mg / L calcium chloride, 300mg / L magnesium sulfate, 0.2mol / L phosphoric acid with pH 7.2 to each liter of distilled water. 0.5 mL of salt buffer constitutes a denitrifying bacteria enriched culture solution. The activated sludge taken from the municipal sewage treatment plant is added to the denitrifying bacteria culture solution to carry out the enrichment culture of denitrifying bacteria. The reactor for culturing denitrifying bacteria is stirred and reacted for 20 hours per day, and settled for 4 hours in a static state. The supernatant is discharged by siphoning method, and then fresh denitrifying bacteria culture solution is added for the next round of operation, running continuously for 70 days to obtain denitrifying bacteria suspension .
[0023] (2) Preparation of polyvin...
Embodiment 2
[0027] Example 2: Continuous denitrification and denitrification of groundwater.
[0028] Pump the groundwater with a nitrate nitrogen concentration of 16~100mg / L from the groundwater storage tank at a flow rate of 50mL / h figure 1 Shown is the inlet at the lower end of the first W frame of the plate-and-frame immobilized cell membrane bioreactor. Groundwater flows upward in the W frame, and flows from the upper outlet on the other side of the W frame to the inlet at the lower end of the next W frame through a duct. The connection between multiple W boxes can be deduced by analogy. The treated groundwater is discharged from the outlet at the upper end of the last W box; at the same time, a pump is used to store an ethanol aqueous solution with an ethanol content of 130-1725 mg / L at a flow rate of 500 mL / min. The tank is conveyed to the inlet of the lower end of the first C frame, and the ethanol aqueous solution flows upwards in the C frame, and flows from the upper outlet of the o...
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