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Plate-frame immobilized cell membrane bioreactor and application thereof

An immobilized cell and bioreactor technology, applied in anaerobic digestion treatment and other directions, can solve the problems of increased ethanol consumption, unfavorable engineering application, slow denitrification rate, etc., to reduce adverse effects, improve processing capacity, and reduce waste. Effect

Inactive Publication Date: 2011-09-07
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is to say, the increase of dissolved oxygen narrows the denitrification area of ​​membrane immobilized cells, therefore, the denitrification rate of this intermittent groundwater denitrification denitrification method is relatively slow, which is not conducive to the development of this groundwater denitrification denitrification method. Engineering applications, and the continuous dissolution of oxygen in the air into the ethanol aqueous solution will also promote the degradation of ethanol by microorganisms and increase the consumption of ethanol
In addition, the effective area of ​​membrane-like immobilized cells in the unit reactor volume of this intermittent groundwater denitrification and denitrification technology is relatively small. Compared with the general MBR, the reactor volume required to achieve the same treatment capacity will be much larger
If the structure of the reactor is not completely changed, it is difficult to increase the processing capacity per unit reactor volume

Method used

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  • Plate-frame immobilized cell membrane bioreactor and application thereof
  • Plate-frame immobilized cell membrane bioreactor and application thereof
  • Plate-frame immobilized cell membrane bioreactor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Construction of a plate and frame type immobilized cell membrane bioreactor.

[0022] (1) Enrichment culture of denitrifying bacteria: add 2000mg / L potassium nitrate, 1500mg / L ethanol, 350mg / L calcium chloride, 300mg / L magnesium sulfate, 0.2mol / L phosphoric acid with pH 7.2 to each liter of distilled water. 0.5 mL of salt buffer constitutes a denitrifying bacteria enriched culture solution. The activated sludge taken from the municipal sewage treatment plant is added to the denitrifying bacteria culture solution to carry out the enrichment culture of denitrifying bacteria. The reactor for culturing denitrifying bacteria is stirred and reacted for 20 hours per day, and settled for 4 hours in a static state. The supernatant is discharged by siphoning method, and then fresh denitrifying bacteria culture solution is added for the next round of operation, running continuously for 70 days to obtain denitrifying bacteria suspension .

[0023] (2) Preparation of polyvin...

Embodiment 2

[0027] Example 2: Continuous denitrification and denitrification of groundwater.

[0028] Pump the groundwater with a nitrate nitrogen concentration of 16~100mg / L from the groundwater storage tank at a flow rate of 50mL / h figure 1 Shown is the inlet at the lower end of the first W frame of the plate-and-frame immobilized cell membrane bioreactor. Groundwater flows upward in the W frame, and flows from the upper outlet on the other side of the W frame to the inlet at the lower end of the next W frame through a duct. The connection between multiple W boxes can be deduced by analogy. The treated groundwater is discharged from the outlet at the upper end of the last W box; at the same time, a pump is used to store an ethanol aqueous solution with an ethanol content of 130-1725 mg / L at a flow rate of 500 mL / min. The tank is conveyed to the inlet of the lower end of the first C frame, and the ethanol aqueous solution flows upwards in the C frame, and flows from the upper outlet of the o...

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Abstract

The invention discloses a plate-frame immobilized cell membrane bioreactor. The bioreactor is characterized in that cover plates are arranged on the outmost layers; the middle part is formed by successively arranging and assembling frames and flat-shaped immobilized cells; the frames include W frames and a C frame; the W frames represent the frames for the underground water to flow; the C frame represents the frame for the ethanol water to flow; the arrangement sequence of the cover plates, the frames and the flat-shaped immobilized cells is as follows: one cover plate, one W frame, one flat-shaped immobilized cell, the C frame, the other flat-shaped immobilized cell, the other W frame and the other cover plate; the lower and upper ends of the W frames and the C frame are respectively provided with inlets and outlets; and bolt holes are arranged around the cover plates and the frames. The bioreactor has the following advantages: the oxygen in the air is avoided from being dissolved in the underground water and ethanol water in the reactor, thus reducing the adverse effects of the dissolved oxygen on denitrification; at the same time, the effective reaction area of the immobilized cell in unit volume and the treatment capability in unit volume are obviously improved; and besides, recycling of the carbon source of denitrification is also realized, thus reducing waste of the carbon source of denitrification.

Description

Technical field [0001] The invention relates to the technical field of drinking water safety protection, in particular to a plate and frame type immobilized cell membrane bioreactor and its application in groundwater biological denitrification and denitrification technology. Background technique [0002] Groundwater is the main source of drinking water in many countries and regions. For example, more than half of the population in the United States uses groundwater as drinking water; 2 / 3 of the drinking water in the Netherlands comes from groundwater. my country's statistics on 21 provinces and cities and 27 major cities show that more than half of the cities use groundwater as their main water source. However, due to the unreasonable use of chemical fertilizers, sewage irrigation and sewage leakage, the concentration of nitrate in groundwater continues to rise. After being ingested into the human body, nitrate is reduced to nitrite in the stomach and intestines, which can conve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28
Inventor 曹国民张立辉盛梅俞益峰史伟伟孟科伟刘勇弟
Owner EAST CHINA UNIV OF SCI & TECH
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