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An expression vector and a method thereof

A technology for expressing vectors and vectors, which is applied in the field of vectors and compounds expressing recombinant soluble proteins, and can solve the problems of recombinant gene silencing, low genetic stability of recombinant genes, and production of recombinant proteins, etc.

Inactive Publication Date: 2011-09-07
AVESTHAGEN
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Problems solved by technology

[0005] Production of recombinant proteins such as TNFR:Fc in mammalian cells may be difficult due to low genetic stability of the recombinant gene and / or silencing of the recombinant gene

Method used

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  • An expression vector and a method thereof
  • An expression vector and a method thereof
  • An expression vector and a method thereof

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Embodiment Construction

[0028] As used herein, the term "scaffold / matrix binding region (S / MAR)" refers to a non-consensus AT-rich DNA element of several hundred base pairs (bp) in length that periodically binds to the protein scaffold or matrix of the nucleus The nuclear DNA of eukaryotic genomes is organized into approximately 60,000 chromatin domains. They are often found in non-coding regions such as flanking regions, chromatin border regions and introns.

[0029] Having "at least 70% homology" refers to DNA in which the nucleotide sequence is at least 70% homologous to the determined sequence as determined by a pairwise alignment of DNA sequences using a matching method such as the BLAST (Basic Local Alignment Search Tool) algorithm.

[0030] As used herein, the term "functional fragment of SEQ ID NO: 1, 2, 3, 4, 5 and 6" refers to a fragment of said sequence of sufficient size to produce the desired effect on expression yield.

[0031] The term "flanking" as used herein means that the sequence...

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Abstract

The present invention relates to vectors and compounds for the expression of recombinant soluble proteins. More particularly, the present invention relates to nucleic acid molecules, expression vectors, and host cells for the expression of recombinant soluble Tumour Necrosis Factor Alpha receptor (TNFR) - Human IgG Fc fusion protein. The invention further relates to methods for preparing recombinant soluble Tumour Necrosis Factor Alpha receptor (TNFR) - Human IgG Fc fusion protein using the host cells transfected with the expression vectors.

Description

field of invention [0001] The present invention relates to vectors and compounds for expressing recombinant soluble proteins. More specifically, the present invention relates to nucleic acid molecules, expression vectors and host cells expressing recombinant soluble tumor necrosis factor alpha receptor (TNFR)-human IgG Fc fusion protein. The invention also relates to a method for preparing recombinant soluble tumor necrosis factor alpha receptor (TNFR)-human IgG Fc fusion protein by using the host cell transfected with the expression vector. Background of the invention [0002] In 1986, the US Food and Drug Administration (FDA) approved the use of human tissue plasminogen activator (tPA, Genentech, CA, USA) protein from mammalian cells for therapeutic purposes. Advances in cell culture and recombinant DNA technology have facilitated the use of genetically engineered cells to express a variety of similar proteins of therapeutic or other economic value. Several monoclonal an...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/67C07K19/00C12N15/13C07K14/715C12N15/12C12N15/62
CPCC07K14/7151C07K2319/30C07K19/00C12N15/11C12N15/62C12N15/67
Inventor V·M·帕特尔S·梅特亚A·维亚斯G·切拉帕
Owner AVESTHAGEN
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