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Preparation method and application of effective component of artemisia sieversiana

A kind of technology of effective parts, Artemisia annua, applied in the fields of chemistry and biomedicine

Active Publication Date: 2019-11-26
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on Artemisia grandis is limited to the chemical composition analysis of Artemisia grandis by Wang Jinlan, Zhao Yanlei, Zhang Qi, Xu Fuchun, etc., and several compounds isolated from it have antiviral and antitumor activities, but Studies on its anti-inflammatory activity have not been reported

Method used

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  • Preparation method and application of effective component of artemisia sieversiana
  • Preparation method and application of effective component of artemisia sieversiana
  • Preparation method and application of effective component of artemisia sieversiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. Take 3.5Kg of dried whole Artemisia grandis, crush it, soak it with petroleum ether, a small polar organic solvent, at room temperature for 7 days, filter to remove the medicinal residues, use a rotary evaporator to remove petroleum ether from the filtrate to obtain the low-polarity part of Artemisia grandis ;

[0019] b. Dissolve and disperse 4 g of the dried Artemisia grandis low-polarity part obtained in step a, add 40 mL of water, extract 3 times with n-hexane, 40 mL each time, evaporate the n-hexane part and the water part of the raffinate to dryness respectively, The water part after evaporation was dissolved in acetonitrile, filtered, and the filtrate was evaporated to dryness to obtain the effective part of Artemisia grandis.

Embodiment 2

[0021] a. Take 100g of dried Artemisia grandis, pulverize it, put it in a 2L stoppered flask, put the flask into an ultrasonic extractor, and use a small polar organic solvent, chloroform, for ultrasonic extraction at room temperature for 3 times, 1.5L each time, and the extraction time for each time For 0.5h, combine the extracts, filter, and concentrate to dryness under reduced pressure in a rotary evaporator to obtain the low-polar fraction of Artemisia grandis;

[0022] b. Dissolve and disperse 4 g of the dried Artemisia grandis low-polarity part obtained in step a, add 40 mL of water, extract 3 times with n-hexane, 40 mL each time, evaporate the n-hexane part and the water part of the raffinate to dryness respectively, The water part after evaporation was dissolved in acetonitrile, filtered, and the filtrate was evaporated to dryness to obtain the effective part of Artemisia grandis.

Embodiment 3

[0024] a. Take 100g of dried Artemisia grandis, crush it, put it in a 2L stoppered flask, put the flask into an ultrasonic extractor, and use a small polar organic solvent acetone to ultrasonically extract at room temperature for 3 times, each time 1.5L, each extraction time For 0.5h, the extracts were combined, filtered, the filtrate was distilled under reduced pressure to recover the solvent, and the concentrated solution was collected at the same time to obtain the low-polarity part of Artemisia grandis;

[0025] b. Dissolve and disperse 4 g of the dried Artemisia grandis low-polarity part obtained in step a, add 40 mL of water, extract 3 times with n-hexane, 40 mL each time, evaporate the n-hexane part and the water part of the raffinate to dryness respectively, The water part after evaporation was dissolved in acetonitrile, filtered, and the filtrate was evaporated to dryness to obtain the effective part of Artemisia grandis.

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Abstract

The invention relates to a preparation method and an application of an effective component of artemisia sieversiana. The effective component is obtained as follows: a low-polarity component is obtained after artemisia sieversiana is subjected to conventional soaking extraction, heating reflux extraction, cold leaching extract, microwave assisted extraction or ultrasonic assisted extraction and removal of a solvent under reduced pressure, the low-polarity component is subjected to extraction with n-hexane after being dissolved and dispersed in water, an extraction part is dissolved in acetonitrile after being dried by distillation, filtrate is dried by distillation after filtration, and the effective component of the artemisia sieversiana is obtained. The effective component of the artemisia sieversiana, which is obtained with the method, proves in vitro that the effective component can be used for preventing and treating inflammation caused by excess of NO, TNF-alpha (tumor necrosis factor-alpha), IL-6 (interleukin-6) and MCP-1 (monocyte chemoattractant protein-1) and can be applied to anti-inflammatory drugs.

Description

technical field [0001] The invention relates to the fields of chemistry and biomedicine, in particular to the use of Artemisia grandis in the preparation of anti-inflammatory drugs. Background technique [0002] Inflammation is a common pathological process, which is a defense-based pathological reaction produced by the body to various harmful stimuli (including physical, chemical, biological, etc.). Normally, inflammation is beneficial and is the body's automatic defense response, but sometimes, inflammation is also harmful. Modern medicine has proved that inflammation is involved in pneumonia, asthma, rheumatoid arthritis, Alzheimer's disease, arterial The occurrence and development of diseases such as atherosclerosis. In addition, inflammation of specific parts or organs can cause serious consequences, such as brain or brain inflammation can compress the vital center, vocal cord inflammation can block the larynx and cause suffocation, and severe myocarditis can affect he...

Claims

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Application Information

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IPC IPC(8): A61K36/282A61P29/00
CPCA61K36/282A61P29/00A61K2236/35A61K2236/33A61K2236/39A61K2236/51A61K2236/333Y02A50/30
Inventor 信学雷崔靖雪段晓梅阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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