Bacillus cereus fermentation method for producing phosphatidase C

A technology of Bacillus cereus and phospholipase, applied in the direction of microorganism-based methods, biochemical equipment and methods, hydrolytic enzymes, etc., can solve the problems of cumbersome separation and extraction process, and achieve clear strain number, simple process and strong production capacity Effect

Inactive Publication Date: 2011-10-05
JIANGNAN UNIV
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Problems solved by technology

[0006] There are also researchers who use genetic engineering to express Pichia pastoris and then produce phospholipase C. However, due to the presence of a large number of inclusion bodies in Pichia pastoris, the subsequent separation and extraction process is very cumbersome.

Method used

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Embodiment Construction

[0017] The present invention is described in further detail below: the method for producing phospholipase C by Bacillus cereus fermentation is through the preparation of 50% egg yolk liquid: get a piece of fresh native egg, wipe the appearance with 75% alcohol, and use sterilized tweezers Gently crack open the eggshell, let the egg white flow out, rinse the egg yolk with sterile water for 1-2 times, use a sterilized glass syringe to draw 10 ml of egg yolk, add it to a 100 ml Erlenmeyer flask filled with 10 ml of sterile water and shake well , sealed, can make 50% egg yolk liquid.

[0018] The composition of the egg yolk plate: NaCl, 0.66%; boric acid, 1.09%; borax, 0.19%; agar, 1.5%; egg yolk liquid, 5%. First weigh NaCl, boric acid, and borax, dissolve them in water, add 1.5% agar, heat to fully dissolve the agar, filter out a small amount of undissolved agar with gauze, replenish water to the correct volume, and then sterilize. Take 10 milliliters of 50% egg yolk liquid and...

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Abstract

The invention discloses a bacillus cereus fermentation method for producing phosphatidase C and belongs to the technical field of enzymic catalytic reaction. The bacillus cereus fermentation method for producing phosphatidase C is utilized to solve the problem of bacterial strains used in the production of phosphatidase C and the problem of complex technology, and comprises the following steps of: inoculating bacillus cereus into the LB medium for culturing to obtain a seed liquid; vaccinating the seed liquid in a fermentation medium consisting of beef extract, peptone, NaCl, water, zincsulfateheptahydrate or ZnCl2, and lecithin or a phosphatide mixture which is the mixture of PC, PE, PI, PS and the like, for culturing to obtain a fermentation broth; and separating the fermentation broth to obtain phosphatidase C. The bacillus cereus fermentation method for producing phosphatidase C can be widely applied to the production of phosphatidase C for plant oil degumming.

Description

technical field [0001] The invention relates to a method for producing phospholipase C by fermentation of bacillus cereus. Background technique [0002] The phospholipase C involved in the present invention is used in the vegetable oil degumming section of oil industry production. Phospholipids in vegetable oils mainly include phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidylserine (PS). Phospholipase C is divided into PC-PLC (specific hydrolysis of PC and PE) and PI-PLC (specific hydrolysis of PI). [0003] Phospholipase C widely exists in animals, plants and microorganisms, but the process of extracting from animals and plants is complicated, and the activity of phospholipase C is greatly lost during the extraction process due to many accompanying substances and complex properties. Microbial fermentation has the advantages of short production cycle and easy separation of fermentation products, so it is the first choice fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12R1/085
Inventor 金青哲周红茹黄健花李进伟宋志华刘元法王兴国
Owner JIANGNAN UNIV
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