Method for detecting Clenbuterol by applying competitive SERS (Surface-Enhanced Raman Scattering) and application thereof

A surface-enhanced Raman, competitive technology, applied in Raman scattering, fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of lack of multiple binding sites, achieve good recovery and specificity, high sensitivity, Simple to use effects

Inactive Publication Date: 2011-10-19
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, clenbuterol, as a typical hormone-like small molecule substance, lacks multiple binding sites, so th...

Method used

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  • Method for detecting Clenbuterol by applying competitive SERS (Surface-Enhanced Raman Scattering) and application thereof
  • Method for detecting Clenbuterol by applying competitive SERS (Surface-Enhanced Raman Scattering) and application thereof
  • Method for detecting Clenbuterol by applying competitive SERS (Surface-Enhanced Raman Scattering) and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1: SERS detection of clenbuterol in pig urine,

[0051] The SERS detection of clenbuterol in pig urine, its steps are as follows ( figure 1 ):

[0052] (1) Preparation of SERS nanoprobes bound to clenbuterol antibody

[0053] Preparation of gold sol (Jiang Yun, Cui Yan, Gu Renao et al. Surface-enhanced Raman spectroscopy research on immunodetection using 4,4′-bipyridine as a labeled molecule[J]. Acta Chem. Sinica, 2006, 64(3): 240~244 ): water preparation 100mL, molar concentration is the chloroauric acid solution (HAuCl4) of 1mM, is placed in the three-necked round bottom flask and is heated to boiling. Then 6 mL of sodium citrate solution (prepared with water) with a molar concentration of 38.8 mM was added. At this time, the color change of the gold colloid is: light yellow-colorless-black-purple-dark red, and after reaching dark red, continue to heat and reflux for 15-20 minutes to obtain gold nanoparticles with an average diameter of 30nm. Then take 1 m...

Embodiment 2

[0063] Example 2: Specific detection of clenbuterol

[0064]In order to illustrate the specificity of the present invention, replace the clenbuterol standard sample with ractopamine and salbutamol standard samples (purchased from Keli Technology Development Company of Guangzhou Analysis and Testing Center) respectively and add them to the pig urine diluent diluted 50 times to prepare respectively The pig urine dilution containing ractopamine and the pig urine dilution containing salbutamol were selected, and 4 different concentrations of pig urine dilution containing ractopamine and salbutamol were selected, respectively 0, 1, 10 and 100pg / mL, instead of containing The pig urine dilution of clenbuterol was tested as the sample to be tested, and the process of Example 1 was repeated. Clenbuterol antibody and ractopamine and salbutamol as the tested antigens are unpaired antibody antigens, which do not have selective recognition. Therefore, ractopamine and albuterol cannot comp...

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Abstract

The invention discloses a method for detecting the Clenbuterol by applying competitive SERS (Surface-Enhanced Raman Scattering) and application thereof, which belong to the technical field of the detection of hormonal micro-molecule substances. In the method disclosed by the invention, a sample to be detected and a SERS nano-probe combined with a Clenbuterol antibody are sequentially dropped on the surface of a substrate with a fixed antigen conjugate; the antigen in the sample to be detected and the antigen in the substrate with the antigen conjugate have an antibody competition reaction with the antibody combined with the SERS nano-probe; after the reaction is finished, the SERS nano-probe which is marked on the substrate with the antigen conjugate is subjected to signal collection through a Raman spectroscope, and data are processed; and finally the quantitative detection is carried out on the Clenbuterol according to the amplitude and standard curve of detection signals. The method disclosed by the invention is convenient to operate, has the advantages of high sensitivity, wide detection interval and high specificity and also has wide application prospects in the fields of food safety, analeptic detection, environment analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of detection of hormone-like small molecular substances, in particular to a method and application for detecting clenbuterol by using competitive surface-enhanced Raman scattering (SERS). Background technique [0002] Clenbuterol (Clenbuterol, CL) is a β-2 adrenoceptor agonist, clinically used as a bronchial antispasmodic drug, initially used in the treatment of asthma. When its application dose reaches 5 to 10 times the therapeutic dose, clenbuterol can enhance muscle development and reduce fat content. In recent years, it has been found that it has been widely used in animal husbandry to increase the yield of lean meat, commonly known as clenbuterol. Human consumption of this kind of animal food will cause a series of side effects, the clinical manifestations are rapid heartbeat, trembling limbs, abdominal pain and dizziness, accompanied by symptoms such as nausea, vomiting, and dyspnea; long-term consumpt...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/64
Inventor 胡勇军朱桂池钟亮高姣
Owner SOUTH CHINA NORMAL UNIVERSITY
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