ITS (internal transcribedspacer region) primer for OLP (oral lichen planus) Candida albicans characteristic sequence and application thereof

A technology for oral lichen planus and Candida albicans, applied in the field of specific primers, can solve problems such as poor repeatability, weak identification ability, and instability

Inactive Publication Date: 2011-11-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although traditional phenotyping has certain research value, it has limitations such as weak discrimination ability, poor repeatability, and instability.

Method used

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Experimental program
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Embodiment 1

[0019] 1 Materials and methods

[0020] 1.1 Research object

[0021] The Candida albicans isolates in the case group came from patients who were diagnosed with OLP in the Department of Stomatology and Mucosal Diseases of the Second Affiliated Hospital of Zhejiang University from 2010.2.16 to 2010.6.1. The diagnosis of OLP patients is based on their typical clinical manifestations and laboratory tests. The main lesions are: most of them are bilaterally symmetrical, and the lines composed of miliary-sized white or off-white papules form a network, ring, dendrites, and plaques Symptomatic lesions, there is no clear boundary between the lesion area and the normal mucosa, between and around the white lines may be normal mucosa or hyperemia, erosion, or even ulcers. The healthy control group had no oral mucosal disease, no clinical symptoms, signs and medical history of any oral mucosal disease.

[0022] The exclusion criteria for the research subjects were: ①Complete denture wear...

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Abstract

The invention provides ITS primers for amplifying OLP Candida albicans characteristic sequences. The invention is characterized in that: the primers are ITS-1:5'-GGAAGTAAAAGTCGTAACAAGG-3', ITS-2:5'-GCTGCGTTCTTCATCGATGC-3'. The beneficial effects of the invention are mainly as follows: ITS primers for amplifying OLP Candida albicans characteristic sequences are provided, and the ITS primers provides a basis for genotyping of OLP Candida albicans, genuinely provides a reliable basis for the formulation of timely and effective clinical control measures at the gene level and lays a foundation for indepth research on Monilia molecular epidemiology.

Description

(1) Technical field [0001] The invention relates to a specific primer for amplifying the characteristic sequence of oral lichen planus Candida albicans and its application. (2) Background technology [0002] Lichen planus (LP) is an abnormal skin and mucous membrane keratosis disease accompanied by chronic superficial inflammation. ), causing great distress to patients. The typical pathology of oral lichen planus (OLP) is hyperparakeratosis of the epithelium, liquefaction of the basal layer, and dense zonal infiltration of lymphocytes in the lamina propria. In the study of the etiology of oral lichen planus, the relationship between Candida and lichen planus has been paid attention to by domestic and foreign scholars very early, but so far no conclusion can be drawn on whether Candida is a commensal bacterium or an initiating pathogen. To conduct a definite study on Candida in oral lichen planus, so as to effectively prevent and treat oral mucosal diseases, it is first nec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C12R1/725
Inventor 何虹杨海萍董研范艳赵丹萍黄剑奇
Owner ZHEJIANG UNIV
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