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Nucleic acid sequence of barley grain B-hordein gene and application thereof

A nucleic acid sequence and seed technology, applied in the field of plant genetic engineering, can solve the problems of limited reports

Inactive Publication Date: 2011-11-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The reported B-hordein genes are mainly from common cultivated barley, ( H. vulgare ), wild lemma barley ( H. turkestanicum ) and wild Chilean barley ( H. chilense ), the reports on the B-hordein gene in barley are very limited

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0027] (1) Using the CTAB method to extract the genomic DNA of highland barley, the extraction steps:

[0028] 1) Take 2g of fresh young leaves, grind them into fine powder with liquid nitrogen, add 2×CTAB extract (2% CTAB; 1.4M NaCl, 0.1 MTris-HCl, PH8.0, 0.1M EDTA, PH8.0) 15 ml, mix well;

[0029] 2) Place in a 65°C water bath for 30-45 minutes, during which time shake gently to mix. After cooling to room temperature, add an equal volume of chloroform:isoamyl alcohol (24:1), mix gently until the supernatant becomes milky, and centrifuge at 4000 rpm for 10 min;

[0030] 3) Take the supernatant, add an equal volume of isopropanol, and place in an ice bath to precipitate DNA;

[0031] 4) Hook out the DNA, wash twice with 70% alcohol, wash once with absolute ethanol, air-dry the DNA, and dissolve in an appropriate amount of 1×TE solution with pH 8.0. Add RNase to a final concentration of 100 μg / μL;

[0032] 5) Under 100V constant voltage, 1% agarose gel electrophoresis for 3...

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Abstract

The invention belongs to the field of plant genetic engineering, and in particular relates to a novel nucleic acid sequence of B-hordein grain protein coding gene from barley of Tibet Plateau, and application thereof. The nucleic acid sequence of the barley grain B-hordein gene contains a gene segment in seq.1, seq.2 or seq.3. The nucleic acid sequence of the barley grain B-hordein gene is used for crop quality improvement.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a nucleic acid sequence of a novel B-hordein grain protein coding gene from highland barley on the Qinghai-Tibet Plateau, and the application of the gene in improving crop quality through biotechnology. Background technique [0002] barley( Hordeum vulgare L.) is an important cultivated crop and a model plant for genetic research in the world. It is widely distributed in agricultural planting areas around the world and has multiple uses such as food, feed, brewing and health care. Highland barley is also called naked barley ( H. vulgare ssp. vulgare ), as an important food and feed crop, plays an extremely important role in the Qinghai-Tibet Plateau region. Its grains are naked, which brings great convenience for processing. In addition, highland barley grains are rich in protein, dietary fiber, vitamin E and β-glucan, etc., which make them have high nutritional va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H1/00
Inventor 余懋群龙海潘志芬邓光兵黄卓韩召雪
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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