Anti-prothymosin α polyclonal antibody and its preparation method and application

A technology of polyclonal antibody and thymosin, which is applied in peptide preparation methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of less than 50% positive rate, many sample collection times, and great influence , to achieve the effect of high specificity and accuracy, good sensitivity and low cost
CN102295701AInactive Publication Date: 2011-12-28XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2011-12-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The anti-thymosin pro-alpha polyclonal antibody and its preparation method and application relate to an anti-thymosin pro-alpha polyclonal antibody. The anti-prothymosin α polyclonal antibody is a polyclonal anti-GST-ProTα antiserum obtained after immunizing New Zealand white rabbits with a fusion protein prepared by using GST-ProTα fusion expression. First prepare the fusion protein GST-ProTα, and then prepare the antibody. It can be used for immunoblotting and ELISA immunoassay of various samples and cells containing ProTα components, and tissue endogenous ProTα. Using the antibody to detect the ProTα level in urine can be used to diagnose urinary system tumors such as bladder cancer, kidney cancer and prostate cancer. The antibody has high titer and strong specificity. Various samples containing ProTα can be detected. Using prothymosin α in urine as a tumor marker for the diagnosis of urinary system cancer. The total process does not exceed 4 hours, and the required amount of samples is small.
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Description

technical field

[0001] The invention relates to an anti-thymosin pro-alpha polyclonal antibody, in particular to an anti-thymosin pro-alpha polyclonal antibody and its preparation method and application. Background technique

[0002] Worldwide, the incidence of bladder cancer ranks ninth among malignant tumors, and it is the most common tumor in the urinary system. In the United States, the incidence of bladder cancer ranks fourth among male malignancies, after prostate cancer, lung cancer, and colon cancer, and ranks ninth among female malignancies. In my country, it ranks 7th in male malignant tumors and 10th in females, with an incidence rate of about 7.4 / 100,000. The degree of malignancy of bladder cancer is not very high, and the 5-year survival rate of early diagnosis and treatment is 93%, but if the diagnosis and treatment cannot be detected in time, the 5-year survival rate of advanced patients is only 6%, and the mortality rate is much higher than that of bladder c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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