Method for culturing in vitro tissues of Semiliquidambar cathayensis
A tissue culture, semi-maple lotus technology, applied in the field of plant tissue culture, to meet the needs and relieve the endangered state.
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[0029] Example: Tissue Culture of Banfenghe
[0030] 1. From the material of the semi-maple lotus to the induction of budding
[0031] After a week of continuous sunny days, the materials were collected at 12-14 noon, and the explants of the plants propagated from the seeds of the excellent mother plant in Tianlong Mountain, Quannan County, Jiangxi were used as explants, washed with detergent and soaked for 10 minutes, and then washed with running water. Rinse for 30 min, place on the operating table, cut into stem segments with 1-2 internodes, treat with 75% ethanol for 25 s, soak with 0.1% mercuric chloride for 10 min, shake continuously during this period, wash 6 times with sterile water, and inoculate On the bud induction medium, the light time is 8~12 h, the light intensity is 3000~5000lx, and the temperature is 25~27℃. After 14 days, axillary buds of 2~3cm can be induced. See attached figure 1 and 2 . The induction rate is 70%;
[0032] The composition of bud induc...
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