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Application of isoquinoline type alkaloids and derivatives thereof to preparation of medicine for inhibiting HPV (Human Papilloma Virus) infection

A technology of drugs and compounds, applied in antiviral agents, drug combinations, sexual diseases, etc., can solve problems such as high expression of oncogenes, and achieve the effect of improving immunity and enhancing autoimmune function.

Inactive Publication Date: 2012-03-28
王芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, existing studies have shown that the pathogenesis of cervical cancer may be due to the imbalance between tumor suppressor genes and proto-oncogenes caused by human papillomavirus infection, which causes high expression of oncogenes and causes disease.

Method used

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  • Application of isoquinoline type alkaloids and derivatives thereof to preparation of medicine for inhibiting HPV (Human Papilloma Virus) infection
  • Application of isoquinoline type alkaloids and derivatives thereof to preparation of medicine for inhibiting HPV (Human Papilloma Virus) infection
  • Application of isoquinoline type alkaloids and derivatives thereof to preparation of medicine for inhibiting HPV (Human Papilloma Virus) infection

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0051] Inhibition of HPV virus by isocydine

[0052] Drug name: isocydine

[0053] Chemical Name:

[0054] 1,2,10-trimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-11-ol

[0055] Drug structure:

[0056]

[0057] To investigate whether isoridine has an inhibitory effect on the expression of HPV virus-related mRNA and protein in HPV-infected human cervical cancer Hela cells and Siha cells, so as to evaluate the application value of isoridine in the treatment of HPV infection.

[0058] experimental method:

[0059] 1. RT-PCR method to observe the changes of gene expression

[0060] The cells were seeded into 96-well plates at 5000 cells / well, and after culturing for 24 h, the old culture medium was discarded, and isorydine containing different drug concentrations (750 mg / L, 1500 mg / L, 3000 mg / L L) Intervention, cultured for 24 h, 48 h, and 72 h respectively, and extracted total RNA with Takara’s RNA extraction kit. First, suck up the medium with a pipette ...

example 2

[0074] The effect of corydine on inhibiting HPV virus

[0075] Drug name: Corydaline

[0076] Chemical Name:

[0077] (6aS)-2,10,11-Trimethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-1-ol

[0078] Drug structure:

[0079]

[0080] To investigate whether cordydine can inhibit the expression of HPV virus-related mRNA and protein in HPV-infected human cervical cancer Hela cells and Siha cells, so as to evaluate the application value of cordydine in the treatment of HPV infection.

[0081] experimental method:

[0082] 1. RT-PCR method to observe the changes of gene expression

[0083] The cells were seeded into 96-well plates at 5000 cells / well, and after 24 h of culture, the old culture medium was discarded, and cordydine containing different drug concentrations (750 mg / L, 1500 mg / L, 3000 mg / L ) intervention, cultured for 24 h, 48 h, and 72 h respectively, and extracted total RNA with Takara’s RNA extraction kit. First, use a pipette tip to suck up the medium...

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Abstract

The invention relates to an application of isoquinoline type alkaloids and derivatives thereof to preparation of a medicine for inhibiting HPV (Human Papilloma Virus) infection, belonging to the field of natural medicine extract preparation and anti-virus and anti-tumor officinal application. The invention provides an application of corydaline, isocorydine, isoboldine, N-methyl hernovine, dicranostigma leptopodum or pharmaceutically acceptably salts or esters to the preparation of the medicine for treating the HPV infection.

Description

technical field [0001] The invention relates to an isoquinoline alkaloid and its derivatives, and uses thereof, belonging to the technical field of natural medicine extracts and pharmaceutical applications. Background technique [0002] Cervical cancer is the second most common malignant tumor in women's death after breast cancer in the world. It is characterized by high malignancy and poor cure rate. Human papillomavirus (HPV) infection is the main risk factor for cervical cancer. It has two encoded subtype chimeric proteins, namely E6 and E7, which can promote cell proliferation, affect the host, and regulate the changes of various related proteins. . The target genes regulated by HPV E6 / E7 proteins are NF-κB molecules, which are closely related to the NF-κB transduction pathway. These transcription factors can initiate protein changes related to innate immune responses, apoptosis and cell proliferation. Moreover, existing studies have shown that the pathogenesis of cerv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/473A61P31/20A61P17/00A61P15/02A61P35/00
Inventor 王芳樊鹏程
Owner 王芳
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