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Preparation method of dedicated microbial flocculant for dairy cattle breeding waste water and strain used in method

A microbial flocculant and aquaculture wastewater technology, applied in the field of flocculants, can solve problems such as high cost and secondary pollution, and achieve the effects of easy operation, no secondary pollution, and harmless to humans and animals

Active Publication Date: 2013-05-01
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a microbial flocculant, which is specially used to treat dairy cow breeding wastewater, which helps to solve technical problems such as high cost and easy to cause secondary pollution in the treatment of dairy cow breeding wastewater

Method used

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  • Preparation method of dedicated microbial flocculant for dairy cattle breeding waste water and strain used in method

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1, the separation of flocculant producing bacteria

[0029] Dilute 10 g of activated sludge taken from a dairy farm by adding 1000 ml of distilled water. The above diluted activated sludge is enriched and cultured in a 30°C constant temperature incubator with a special NA medium plate, and the culture time is 30 hours. Select a single colony that grows vigorously, has a smooth surface, is a little transparent, and is viscous, and repeatedly streaks and separates to obtain a single colony until the microscopic examination confirms that the obtained bacteria are relatively pure single colonies.

[0030] The above-mentioned special NA medium plate is composed of basic NA medium and dairy cow breeding wastewater. The components of the basic NA medium are: peptone 10g, beef extract 3g, NaCl 5g, agar 20g and H 2 O 1000ml, add 100ml of dairy cow breeding wastewater with a COD concentration of 5000mg / L to the above-mentioned basal NA medium per liter, and adjust the ...

Embodiment 2

[0041] Embodiment 2, a kind of production method of microbial flocculant

[0042] 1) Seed liquid culture: Inoculate a loop of the isolated Bacillus amyloliquefaciens ZJ28#Bacillus amyloliquefaciens ZJ28#CCTCC No: M2011419 into a 250ml Erlenmeyer flask containing 100ml of special liquid medium, at 30°C at 200r / min Rotating speed shaking culture for 30h, to obtain the strain seed liquid.

[0043] The above-mentioned special liquid medium used is composed of basic liquid medium and dairy cow breeding wastewater. The composition of the basic liquid medium is: peptone 10g, beef extract 3g, NaCl 5g and H 2 01000ml, add 100ml COD concentration to be 7000~8000mg / L dairy cow breeding waste water on above-mentioned every L basic liquid medium, and adjust pH=7.4~7.6 with 1mol / L NaOH, get special liquid medium.

[0044] 2), the strain seed solution was inoculated into the fermentation medium at a volume ratio of 2.0:100, the initial pH value was 7.3 (natural pH value), the temperature wa...

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Abstract

The invention discloses bacillus amyloliquefaciens, which, namely Bacillus amyloliquefaciens ZJ28#, is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China, on November 24, 2011. The invention also discloses an application of the bacillus amyloliquefaciens, which is used for preparing a dedicated microbial flocculant for dairy cattle breeding waste water. The invention discloses a preparation method of the dedicated microbial flocculant for dairy cattle breeding waste water. The flocculant is dedicated for treating the dairy cattle breeding waste water, and is advantageous to solving the technical problems of high cost, easily caused secondary pollution and the like in dairy cattle breeding waste water treatment.

Description

technical field [0001] The invention relates to a flocculant, in particular to a method for producing a microbial flocculant specially used for treating dairy cow breeding wastewater; the invention also relates to a method for using the microbial flocculant. Background technique [0002] With the continuous development of the modern economy, the animal husbandry has also developed rapidly, and large-scale animal husbandry farms continue to emerge, which have brought about the deterioration of the environment while developing the rural economy and improving the living standards of residents-aquaculture wastewater pollution . Among them, dairy farming wastewater has the characteristics of mixed solid and liquid, high suspended solids, high organic matter content, imbalanced carbon-nitrogen ratio, and difficult treatment, which seriously endangers the environment and human health. [0003] Flocculation technology is an economical and simple water treatment technology used at h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/07C02F103/20
Inventor 胡秀芳黄志成卢燕赵凯鹏曹争易刘琼瑶
Owner ZHEJIANG SCI-TECH UNIV