Simple inoculation method for paraffin tissue chip

Abstract

The invention provides a simple inoculation method for a paraffin tissue chip. The method is characterized by comprising the steps of: conducting sampling on a pathologic tissue wax block; heating the wax to a melting state, placing a wax pattern preparation frame on a plastic wax template, pouring wax into the wax pattern preparation frame for polymerization and cooling, and removing the plastic wax template; putting the obtained wax pattern and the wax pattern preparation frame in an incubator, and taking the wax pattern out after it softens, carrying out drilling with the inoculation scope of the wax pattern, and planting a chip tissue into a hole corresponding to the wax pattern; placing the inoculation face of the obtained wax pattern on a flat ironing platform, after melting of the wax, stopping heating and leaving the wax to natural cooling; reheating the flat ironing platform, and removing the wax pattern and the wax pattern preparation frame; placing the wax pattern and the wax pattern preparation frame into a refrigerator, then taking them out and separating them, and slicing the obtained wax pattern so as to obtain a paraffin tissue chip. The method of the invention has the advantages of low cost, convenience, rapidity, high yield, and sampling diversity, and can be popularized to every pathology department as well as related research units.

Description

technical field [0001] The invention provides a simple inoculation method for paraffin tissue chips, which is used for preparing tissue chips and belongs to the technical field of tissue chips. Background technique [0002] Tissue chip technology is the development and extension of gene chip technology. Like cell chip, protein chip and antibody chip, it belongs to a special biochip technology. Tissue chip technology can arrange dozens or even thousands of clinical tissue specimens of different individuals in a pre-designed sequence for analysis and research. It is a high-throughput, multi-sample analysis tool. Tissue chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can not only be combined with traditional pathological techniques, histochemical and immunohistochemical techniques, but also with DNA, RNA, protei...

AI Technical Summary

Problems solved by technology

[0006] However, the preparation of tissue chips according to the above-mentioned method has the following disadvantages at present: first, the production cost of existing tissue chips is very high

In addition to special instruments and equipment (hundreds of thousands of yuan), the cost of making a tissue chip is more than 10,000 yuan, and the unit price of each slice is 50-100 yuan; The source is limited; thirdly, the chip prepared according to the existing equipment and method has only about 50 slices, because the sample is made by punching holes on the original wax block, and the tissue thickness is limited; fourthly, although the high-density chip has a large throughput, However, due to the small size of each tissue, it is difficult to provide information on the tissue structure; fifth, only in large-scale research units, there is funding and conditions for tissue chip-related research, which is difficult to promote