Method for extracting bile acid from bilirubin leftovers

Abstract

The invention relates to a method for extracting bile acid from bilirubin leftovers. The method comprises the following steps of: adding alkali into leftovers of bile from which bilirubin is completely extracted serving as a raw material for saponifying; adding hydrogen peroxide for decolorizing; and adding an acid for separating and washing. In the invention, the aim of purifying is fulfilled by dissolving impurities such as proteins and fats in bile cream into an alkaline liquor in a high-temperature saponifying way, further removing impurities in a chemical decolorizing way and dissolving and washing out water-soluble impurities in a pH value adjusting way. The method for producing bile acid disclosed by the invention has the advantages of easiness for operating, freeness from toxic and harmful organic solvent, realization of large-scale industrial production, high product purity (bile acid is more than 98 percent), and total yield of over 40 percent.

Description

technical field [0001] The invention relates to a method for extracting bile acid. Background technique [0002] With the advancement of science and technology and the increasingly fierce competition and challenges in the research and development of new drugs at home and abroad, the focus of new drug research is increasingly shifting to the development and utilization of active ingredients in traditional Chinese medicine and natural medicines. The active ingredient of traditional Chinese medicine is the material basis for its medicinal effect, and its in-depth study is the key to the modernization of traditional Chinese medicine. [0003] Since the last century, the research on bile and bile acids has been a hot spot in the field of chemistry and medical science. The research focus has shifted from the initial separation and structure identification to the exploration of pharmacological effects, but the main object of research is free bile acids. With the development of med...

AI Technical Summary

Problems solved by technology

[0008] The disadvantages of the above two methods are: although all the proteins in the bile can be removed, cholesterol and neutral fat are still retained in the bile acid, so the product has a low purity

The disadvantages of this method are: complex operation, large amount of methanol, no obvious stratification when extracting cholesterol with chloroform and methanol, difficult to master, prone to errors, difficult to achieve the purpose of separating cholesterol and lipid, and the cost is too high

The disadvantages of this method are: the price of ethanol is high, and it cannot be used repeatedly, and the production cost is relatively high. Furthermore, the purity of the obtained bile acid is not high, and the impurities cannot be completely removed, so it is difficult to achieve the purpose of separating cholesterol and lipids.