Determination method of ammonia (ammonia ion) and ammonia (ammonia ion) diagnosis / determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0136] Embodiment 1: Determination of ammonia (ammonia ion) content in plasma
[0137] 1) Preparation of standard samples
[0138] Dissolve a certain amount of ammonia salt in water or buffer, and then adjust the ammonia concentration to 100 μmol / L;
[0139] 2) Pretreatment of the sample to be tested
[0140] Plasma samples are used as samples to be tested without pretreatment;
[0141] 3) Blank sample
[0142] Described water or buffer is used as blank sample, and its ammonia concentration is 0 micromol / liter;
[0143] B. Preparation of reagent solution:
[0144] The ammonia diagnostic / assay reagent of this embodiment is a single-dose reagent, which contains:
[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0146] Glycerol 1mol / L
[0147] NADPH 0.25mmol / L
[0148] Glutamate oxidase 16000U / L
[0149] Carbamoyl phosphate synthase 18000U / L
[0150] Malonate-semialdehyde dehydrogenase 12000U / L
[0151] Hydrogen peroxide 10mmol / L
[0152] 2...
Embodiment 2
[0169] Example 2: Determination of ammonia (ammonia ion) content in plasma
[0170] 1) Preparation of standard samples
[0171] Dissolve a certain amount of ammonia salt in water, and then adjust the ammonia (ammonia ion) concentration to 100 μmol / L as a standard sample;
[0172] 2) Preparation of samples to be tested
[0173] Plasma samples are used as samples to be tested without pretreatment;
[0174] 3) Blank sample
[0175] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromol / liter;
[0176] B. Preparation of reagent solution:
[0177] Ammonia (ammonia ion) diagnostic / measurement reagent is a two-agent reagent, which contains:
[0178] Reagent 1 consisting of the following:
[0179] Phosphate buffer 100mmol / L
[0180] NADH 0.25mmol / L
[0181] Hydrogen peroxide 10mmol / L
[0182] 2-ketoglutaric acid 10mmol / L
[0183] Adenosine diphosphate 5mmol / L
[0184] Carbamoyl phosphate 10mmol / L
[0185] Acetyl-CoA 10mmol / L;
...
Embodiment 3
[0208] Example 3: Determination of ammonia (ammonia ion) content in plasma samples
[0209] The operation mode of this embodiment is the same as that of Embodiment 2, except that this embodiment:
[0210] B. Preparation of reagent solution:
[0211] Ammonia (ammonia ion) diagnostic / measurement reagent is a three-dose reagent, which contains:
[0212] Reagent 1 consisting of the following:
[0213] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0214] NADH 0.25mmol / L
[0215] Hydrogen peroxide 10mmol / L
[0216] 2-ketoglutaric acid 10mmol / L
[0217] Adenosine diphosphate 5mmol / L
[0218] Carbamoyl phosphate 10mmol / L
[0219] Acetyl-CoA 10mmol / L;
[0220] Reagent 2 consisting of the following:
[0221] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0222] Sodium chloride 2mol / L
[0223] Carbamoyl phosphate synthase 28000U / L
[0224] Malonate-semialdehyde dehydrogenase 50000U / L;
[0225] Reagent 3 consisting of the following...
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