Method for detoxification of lily bulbs

A technology of lily and ribavirin, which is applied to the field of detoxification of lily bulbs, can solve problems such as economic losses in the lily industry, and achieve the effect of improving practical operability

Active Publication Date: 2012-06-27
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using biological, physical and chemical methods to prevent virus da...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment one: a kind of method for lily bulb detoxification, comprises the following steps:

[0025] (1) Treat susceptible lily bulbs at 4°C for 50 days;

[0026] (2) Select susceptible lily bulbs with a bud length of 4 cm, peel off the outer scales, and leave a bulb disc with 3 inner scales, and put the bulb discs into an incubator for pretreatment. The pretreatment conditions are: light intensity 1500Lux, temperature 35°C, treatment time 3 days;

[0027] (3) After the pretreatment, the bulb discs were alternately treated with light and darkness for 28 days, and then treated with 7 hours of darkness after every 15 hours of light. The light treatment temperature was 37°C, the light intensity was 1500Lux, and the dark treatment temperature was 24°C. ;

[0028] (4) Ribavirin treatment: Select a bulb disc with normal growth, peel off the 0.8cm stem tip and incubate it on the differentiation medium for 14 days, the culture temperature is 24°C, the light intensity is 150...

Embodiment 2

[0030] Embodiment two: a kind of method for lily bulb detoxification, comprises the following steps:

[0031] (1) Treat susceptible lily bulbs at 8°C for 45 days;

[0032] (2) Select susceptible lily bulbs with a bud length of 8 cm, peel off the outer scales, and leave a bulb disc with 5 inner scales, and put the bulb discs in an incubator for pretreatment. The pretreatment conditions are: light intensity 2000Lux, temperature 40℃, treatment time 2 days;

[0033] (3) After the pretreatment, the bulb discs were alternately treated with light and darkness for 30 days, and then treated with 8 hours of darkness after every 16 hours of light. The light treatment temperature was 39°C, the light intensity was 2000Lux, and the dark treatment temperature was 26°C. ;

[0034] (4) Ribavirin treatment: Select a bulb disc with normal growth, peel off the 1.2cm shoot tip and incubate it on the differentiation medium for 16 days, the culture temperature is 27°C, the light intensity is 2000L...

Embodiment 3

[0036] Embodiment three: a kind of method for lily bulb detoxification, comprises the following steps:

[0037] (1) Treat susceptible lily bulbs at 6°C for 48 days;

[0038] (2) Select susceptible lily bulbs with a bud length of 6 cm, peel off the outer scales, and leave a bulb disc with 4 inner scales, and put the bulb discs into an incubator for pretreatment. The pretreatment conditions are: light intensity 1800Lux, temperature 36°C, treatment time 2.5 days;

[0039](3) After the pretreatment, the bulb discs were alternately treated with light and dark for 29 days, and then treated with 7.5 hours of darkness after every 15.5 hours of light. The light treatment temperature was 38°C, the light intensity was 1800Lux, and the dark treatment temperature was 25°C. ;

[0040] (4) Ribavirin treatment: Select a bulb disc with normal growth, peel off a 1.0cm shoot tip and incubate it on a differentiation medium for 15 days, the culture temperature is 25°C, the light intensity is 180...

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PUM

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Abstract

The invention relates to a method for detoxification of lily bulbs. The method for detoxification of lily bulbs is characterized by comprising the following steps of 1, carrying out low-temperature treatment on infected lily bulbs, 2, stripping outer scales of the infected lily bulbs, putting the outer scale-free lily bulbs into an incubator, and carrying out pretreatment, 3, alternatively carrying out illumination treatment and dark treatment on the pretreated lily bulbs for 28 to 30 days, wherein each one illumination treatment period is in a range of 15 to 16 hours; each one dark treatmentperiod is in a range of 7 to 8 hours; every cycle of the dark treatment is carried out after the corresponding cycle of the illumination treatment; an illumination treatment temperature is in a rangeof 38+/-1 DEG C; illumination intensity is in a range of 1500 to 2000Lux; and a dark treatment temperature is in a range of 25+/-1 DEG C, and 4, selecting the lily bulbs which are obtained by the step 3 and grow normally, taking bulb tips having sizes of 0.8 to 1.2 centimeters, inoculating a differentiation medium with the bulb tips, and carrying out culture under the conditions of culture time of 14 to 16 days, illumination intensity of 1500 to 2000Lux, a culture temperature of 25+/-2 DEG C and an illumination period of 11 to 12 hours per day to obtain bulblet buds. The method for detoxification of lily bulbs utilizes the combination of multiple detoxification methods, improves practical operability of detoxification processes, and has a real meaning for technology popularization.

Description

technical field [0001] The invention relates to a method for detoxifying lily bulbs, and belongs to the technical field of flower cultivation and production. Background technique [0002] Lily belongs to the genus Lilium of the family Liliaceae. It is an important cut flower with many cultivars and the planting area is expanding day by day. The history of commercial cultivation of lily cut flowers in my country is relatively short, and the overall production level and technology lag behind countries such as the Netherlands, the United States, and Japan, and currently mainly rely on imported bulbs for production. Lily bulbs are expensive and susceptible to viruses, especially after multi-band self-propagation, the virus accumulates and spreads, and in addition to the lack of strict plant virus quarantine control technology, only the virus accumulates and spreads in the lily body. Due to the lack of virus-free high-quality provenance The virus infection rate of self-propagati...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 惠峰赵湘
Owner WUXI MISHO ECOLOGY LANDSCAPE
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