Extracting process of biocontrol strains and toxin of biocontrol strains and purposes of biocontrol strains and toxin of biocontrol strains

Abstract

The invention discloses an extracting process of biocontrol strains being fruit pythium aphanidermatum and fruit pythium aphanidermatum toxin for prevention and treatment of skyblue broomrape herb. The extracting process comprises enabling the fruit pythium aphanidermatum preserved on an inclined plane to be activated on a PDA panel, picking bacterium blocks, collecting hypha after the bacterium blocks are shaken and cultured in a PD culture medium, performing rotating steaming concentration after ethyl acetate solution is extracted in an ultrasound circulating extraction meter, utilizing NaHNO3 solution to extract crude extract, transferring the crude extract into ethyl ether solution, collecting aqueous phase, adjusting pH to utilize petroleum ether solution to extract extract, collecting the aqueous phase again and performing extraction through ethyl acetate, and performing constant volume of methanol after the ethyl acetate is decompressed and concentrated. The invention further discloses purposes of biocontrol strains and the toxin of the biocontrol strains, and the purposes are for controlling and preventing root parasitism weed skyblue broomrape herb. The extracting process is suitable for unique geological weather conditions of Sinkiang, is used for effectively preventing root parasitism weed skyblue broomrape herb of cash crops, and is suitable for industrial massive production, economical, practical and low in cost.

Description

technical field [0001] The present invention relates to a biocontrol strain and its toxin application for controlling the occurrence of parasitic weeds for processing tomato, melon, sunflower and other economic crops and the extraction process of the strain toxin suitable for industrial production, especially a The application of Pythium melon and fruit and its toxin, and the extraction process and application of the toxin of the strain. Background technique [0002] The processed tomatoes grown in Xinjiang are high in red pigment and oryzanol, less moldy, and have good viscosity. They are the first-class raw materials for processing tomato products, so tomato products are very competitive in the international market. In 2009, nearly 1.2 million mu of tomatoes were planted and processed in Xinjiang, accounting for more than 75% of the national planting area. Xinjiang has become the most important tomato planting and processing base in my country. Hami melon is a famous spec...

AI Technical Summary

Problems solved by technology

However, these measures are troublesome, and long-term crop rotation is difficult to implement in many areas. Spraying natural stimulants or planting trap plants cannot induce all the seeds of Lidang at one time, and Lidang's reproductive ability is very strong. Once a small amount survives, it can be Produces a large number of offspring, so the control effect is not obvious

Another way to prevent and control Lidang is the prevention and control after germination, such as screening resistant varieties of Lidang, manually pulling them out, adding organic fertilizers such as chicken manure, applying engine oil, or using Doul+glyphosate+water at a ratio of 1:3 : 40 or 1 : 4 : 50 after recombination, smearing and other measures of Lidang are used to prevent and control, but these measures have disadvantages such as difficult operation or high cost, so it is difficult to implement

If it is very difficult and time-consuming to breed disease-resistant varieties, once the listed physiological race changes, the control effect will be lost

However, smearing or spraying with herbicides has the disadvantages of labor-intensive, labor-intensive and poor safety.

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