Method for producing nervonic acid by utilizing transgenic microalgae

A neural acid and transgenic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of fast growth, reduced production costs, and increased production

Inactive Publication Date: 2012-07-11
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, until now, there is no large-scale production technology worldwide, and small amounts of nervonic acids are mainly extracted from limited plant resources, or in extremely low levels during the production of erucic acid from rapeseed oil , which is in stark contrast to the fact that DHA can be produced on a large scale by microalgae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the garlic fruit tissue, extract the total RNA as a template, use the designed primer RT-PCR to get the mRNA of 3-ketoacyl-CoA synthase, use the RACE technology to get the full length, connect to the vector, and use the gene gun to inject the vector into the rich Oil new green algae cells, screening transformants, expanding transformants, collecting microalgae cells, extracting oil, hydrolyzing oil, and analyzing fatty acid composition with GC-MS, the results showed that the oil content of microalgae was 45% (to the dry weight of cells), and the neural acid accounted for More than 40% of total fatty acids.

Embodiment 2

[0018] Take the maple tissue, extract the total RNA, use the designed primer RT-PCR to get the mRNA of 3-ketoacyl-CoA synthase, use RACE technology to get the full length, connect to the vector, and use Agrobacterium to transfer the vector into the pellet Algae cells, screening transformants, expanding transformants, collecting microalgae cells, extracting oil, hydrolyzing oil, and analyzing fatty acid composition by GC-MS, the results show that the oil content of microalgae is 30% (to the dry weight of cells), and nervous acid accounts for the total fatty acid 35% or more.

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Abstract

The invention relates to a method for producing nervonic acid by utilizing transgenic microalgae. The method comprises the steps that the gene of a key enzyme, namely 3-ketoacyl CoA (coenzyme A) synthase, with high nervonic acid production capacity of fatty acid prolonged anabolism pathway in organism is transferred to a host microalgae cell, so that the microalgae is endowed with or enhanced in the nervonic acid synthesis capability; by cultivating the transgenic microalgae and promoting oil and fat accumulation of the microalgae, oil and fat with high content of nervonic acid can be extracted from the cultivated microalgae cells, and further, nervonic acid can be extracted from the oil and fat.

Description

technical field [0001] The invention relates to a production method of nervonic acid, in particular to a method for producing nervonic acid through transgenic microalgae, and belongs to the technical field of nervonic acid gene production. Background technique [0002] Nervous acid, chemical name cis-15-tetradecanoic acid, is a long-chain monounsaturated fatty acid of the ω9 series, and its molecular formula is C 24 h 46 o 2 , the relative molecular weight is 366.6, it is a white flaky crystal at room temperature, soluble in alcohol, insoluble in water, and its melting point is 39~40°C. [0003] Nervous acid is an important component of brain nerve cells and nerve tissues. In mammals, nervonic acid is widely distributed in the sphingolipids of vertebrate tissues, where nervonic acid is combined with sphingosine through an amide bond. It is an important component of the white matter of the animal brain and peripheral nervous tissue. [0004] Studies have shown that nervon...

Claims

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Application Information

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IPC IPC(8): C12N15/79C12N1/13C12P7/64C12R1/89
Inventor 李雁群
Owner GUANGDONG OCEAN UNIVERSITY
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