Excitatory amino-acid transporter-3 gene cRNA in-situ hybridization probes and preparation method thereof

Abstract

The invention discloses excitatory amino-acid transporter-3 (EAAT3) gene cRNA in-situ hybridization probes and a design method thereof. The method comprises the steps that: (1) specific primers of EAAT3 are designed according to a Gene bank sequence of EAAT3; an 292bp EAAT3 fragment is amplified by using the primers, and the fragment is adopted as a specific probe sequence of EAAT3; (2) EAAT3 cRNA in-situ hybridization probe plasmids are constructed; the probe plasmids are used for converting bacteria; the bacteria are cultivated; and the bacteria are sequenced; (3) EAAT3 cRNA in-situ hybridization probes are prepared; and (4) EAAT3 cRNA probe in-situ hybridization is detected.

Description

Technical field: [0001] The invention belongs to the field of biomedicine and relates to a probe and a design method thereof, in particular to a glutamate transporter-3 gene cRNA in situ hybridization probe and a preparation method thereof. Background technique: [0002] Glutamate is the most important neurotransmitter in the central nervous system of mammals. It mainly binds to two families of glutamate receptors, ionophilic and metabotropic, to activate them. If the concentration of glutamate in the extracellular area is too high, excessive excitation of glutamate receptors can lead to nerve cell death. Since there is no enzyme that can decompose glutamate outside the cell, the released glutamate is almost entirely dependent on neurons and glia The uptake of glutamate transporter (EAAT) on the cell membrane keeps the concentration relatively stable, and EAAT also provides a source of glutamate for many metabolic pathways in the body. Glutamate transporters are divided int...

AI Technical Summary

Problems solved by technology

In terms of morphological research, the detection of the mRNA level of the glutamate transporter-3 gene in living animals shows that it is still blank

Images