The invention discloses a recombinant chimeric protein carrying a hepatitis B virus epitope and the preparation thereof, particularly a recombinant chimeric protein respectively carrying a hepatitis B virus S82, C19, S125, S2[8], S1[93] or C117 epitope, and a recombinant chimeric protein simultaneously carrying hepatitis B virus S82, C19, S125, S2[8], S1[93] and C117 epitopes, and through the immunology WesternBlot detection, each recombinant protein not only has the antigen reactivity and the immunogenicity of a VP6F protein, but also has the antigen reactivity and the immunogenicity of the HBV (hepatitis B virus) epitope, does not have infectivity and the side effects of virulence atavism, the intussusception and the like, and has important value for developing new generation RV (Rota Virus)/HBV recombinant chimeric vaccine and reagent.