Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application

A technology of hepatitis B virus and liver cancer, which is applied in the antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application field, to achieve the effect of strengthening the fight against hepatitis B liver cancer

Active Publication Date: 2021-06-29
郑振宜
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main technical difficulties are: (1) HBV-HCC has highly heterogeneous tumor antigens with diversity, complexity, and variability, which can help cancer cells easily escape the monitoring of the immune system; (2) HBV-HCC The immunosuppressive microenvironment formed inside makes it difficult for immune cells to break through its "protective net"

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application
  • Antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application
  • Antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Peripheral blood DC and T cell isolation and expansion culture.

[0033] (1) Obtain peripheral blood mononuclear cells: use mcs+ portable blood cell collection machine, operate according to the procedures set in the manual, peripheral blood circulation 2000 ~ 2500ml, collect peripheral blood mononuclear cells 50ml, centrifuge at 500G for 20 minutes in a 5810R centrifuge, and collect the upper plasma Aliquot into 1.5 ml centrifuge tubes and freeze for routine biochemical testing. After diluting the lower layer of cells with normal saline 1:1, transfer them into a 50ml centrifuge tube containing 25ml of lymphocyte separation medium, use a 5810R centrifuge at 450G for 30 minutes, carefully absorb the middle white cell layer with a disposable pipette, and transfer it into a 50ml centrifuge tube containing 40ml of normal saline. Tube, gently pipette evenly, centrifuge at 250G for 15 minutes, discard the supernatant, add 45ml of normal saline, gently pipette evenly, centri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an antigen presentation signal group of hepatitis B virus (HBV) antigen peptide combined with liver cancer cell antigen information and its application. The signal group consists of HBV core antigen 18-27 peptide segment, HBV pre-S2 antigen 44-53 peptide segment and The composition of human liver cancer cell line HepG2 lysate, the mass ratio of HBV core antigen 18-27 peptide, HBV pre-S2 antigen 44-53 peptide and human liver cancer cell line HepG2 lysate is 0.5~1:0.5~1:1 ~2. The DC cells induced by this signal group become a DC vaccine carrying multiple specific HBV-associated liver cancer antigen signals, which can activate the proliferation and function of tumor-specific T cells, thereby strengthening its role in fighting HBV liver cancer.

Description

technical field [0001] The present invention relates to the technical field of specific cellular immunotherapy for hepatitis B virus-associated liver cancer, in particular, it relates to an antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application, and the specific application is to activate dendritic cells Application of induced T cells. Background technique [0002] In stark contrast to the continuous decline in overall cancer mortality worldwide, the incidence and mortality of liver cancer are increasing year by year. The latest epidemiological data show that the annual incidence of liver cancer is 782,000, and the number of deaths is 745,000. The average 5-year survival rate is less than 5%. , the overall survival time is only 10 months; my country accounts for 55% of liver cancer in the world, and has become the country with the largest incidence and death of liver cancer. More than 85% ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K39/29A61P1/16A61P31/20A61P35/00C12N5/0783
CPCA61K39/12C12N5/0636A61K2039/70C12N2730/10134A61K39/4644A61K2239/53A61K39/4611A61K39/464838A61K39/4622A61K2239/31A61K39/4615Y02A50/30
Inventor 郑振宜
Owner 郑振宜
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com