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Antigen presenting signal group combining hepatitis B virus (HBV) antigen peptides and liver cancer cell antigen information, and applications

A technology of hepatitis B virus and liver cancer cells, applied in the direction of virus antigen components, viruses, animal cells, etc., to achieve the effect of strengthening the fight against hepatitis B liver cancer

Active Publication Date: 2017-12-22
郑振宜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main technical difficulties are: (1) HBV-HCC has highly heterogeneous tumor antigens with diversity, complexity, and variability, which can help cancer cells easily escape the monitoring of the immune system; (2) HBV-HCC The immunosuppressive microenvironment formed inside makes it difficult for immune cells to break through its "protective net"

Method used

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  • Antigen presenting signal group combining hepatitis B virus (HBV) antigen peptides and liver cancer cell antigen information, and applications
  • Antigen presenting signal group combining hepatitis B virus (HBV) antigen peptides and liver cancer cell antigen information, and applications
  • Antigen presenting signal group combining hepatitis B virus (HBV) antigen peptides and liver cancer cell antigen information, and applications

Examples

Experimental program
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Embodiment 1

[0032] 1. Peripheral blood DC and T cell isolation and expansion culture.

[0033] (1) Obtain peripheral blood mononuclear cells: use mcs+ portable blood cell collection machine, operate according to the procedures set in the manual, peripheral blood circulation 2000 ~ 2500ml, collect peripheral blood mononuclear cells 50ml, centrifuge at 500G for 20 minutes in a 5810R centrifuge, and collect the upper plasma Aliquot into 1.5 ml centrifuge tubes and freeze for routine biochemical testing. After diluting the lower layer of cells with normal saline 1:1, transfer them into a 50ml centrifuge tube containing 25ml of lymphocyte separation medium, use a 5810R centrifuge at 450G for 30 minutes, carefully absorb the middle white cell layer with a disposable pipette, and transfer it into a 50ml centrifuge tube containing 40ml of normal saline. Tube, gently pipette evenly, centrifuge at 250G for 15 minutes, discard the supernatant, add 45ml of normal saline, gently pipette evenly, centri...

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Abstract

The present invention discloses an antigen presenting signal group combining hepatitis B virus (HBV) antigen peptides and liver cancer cell antigen information, and applications, wherein the signal group comprises a HBV core antigen 18-27 peptide fragment, a HBV PreS2 antigen 44-53 peptide fragment and a human liver cancer cell line HepG2 lysate according to a mass ratio of 0.5-1:0.5-1:1-2. According to the present invention, DC cells induced by the signal group become the DC vaccine carrying the antigen signals of multi-specific hepatitis B virus-associated liver cancers, and can activate the proliferation and the function of tumor-specific T cells so as to enhance the anti-hepatitis B liver cancer effect.

Description

technical field [0001] The present invention relates to the technical field of specific cellular immunotherapy for hepatitis B virus-associated liver cancer, in particular, it relates to an antigen presentation signal group of hepatitis B virus antigen peptide combined with liver cancer cell antigen information and its application, and the specific application is to activate dendritic cells Application of induced T cells. Background technique [0002] In stark contrast to the continuous decline in overall cancer mortality worldwide, the incidence and mortality of liver cancer are increasing year by year. The latest epidemiological data show that the annual incidence of liver cancer is 782,000, and the number of deaths is 745,000. The average 5-year survival rate is less than 5%. , the overall survival time is only 10 months; my country accounts for 55% of liver cancer in the world, and has become the country with the largest incidence and death of liver cancer. More than 85% ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/29A61P1/16A61P31/20A61P35/00C12N5/0783
CPCA61K39/12C12N5/0636A61K2039/70C12N2730/10134A61K39/4644A61K2239/53A61K39/4611A61K39/464838A61K39/4622A61K2239/31A61K39/4615Y02A50/30
Inventor 郑振宜
Owner 郑振宜
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