19 results about "Bbb permeability" patented technology

The invention discloses a black phosphorus-methylene blue nano composition for improving blood brain permeability and a synthesis method thereof. The synthesis method comprises the following steps: step 1, mixing black phosphorus nanosheets with polyethylene glycol for reaction; step 2, carrying out centrifugal cleaning on the reaction liquid in the step 1, and collecting a lower-layer reactant; step 3, adding aminated superparamagnetic nanoparticles and methylene blue into the lower reactant obtained in the step 2 for reaction; and 4, carrying out centrifugal cleaning on the reaction liquid obtained in the step 3, and collecting a lower-layer reactant. The invention also discloses the black phosphorus-methylene blue nano composition synthesized by the synthesis method, the drug loading rate of methylene blue in the composition exceeds 60%, and the composition has good biological safety, is non-toxic to cells and mice, is very simple and convenient in preparation process, and has a wide application prospect in the aspect of improving the blood-brain barrier permeability.

The present application relates to a kind of hyperbranched polymer, nano preparation and its preparation method and application, belong to functional medical material and nanotechnology field.The hyperbranched polymer of the present application can be broken in ROS environment Release dihydrolipoic acid, play the role of antioxidant, be reduced to lipoic acid in vivo, and then play its role as coenzyme, participate in mitochondrial tricarboxylic acid cycle, restore mitochondrial function, thereby play the role of antioxidant and play the role of treating ischemic stroke.The nano preparation provided by the present application can be quickly distributed to brain in a short time, temporarily increase the paracellular permeability of BBB by inhibiting the expression of multi-drug resistance protein P-gp and ATP-dependent drug efflux protein on BBB, and the process of opening BBB by borneol is physiological and reversible, without destroying the structural integrity of blood-brain barrier.

The application discloses a kind of compound screening method and system for regulating blood-brain barrier permeability, it is related to medical screening technical field, the specific steps of the method are as follows: S100, collect blood-brain barrier related cell multi-omics data to form original set;S200, data is preprocessed and standardized;S300, molecular network is constructed by cross-omics analysis, and key target is screened;S400, target information is integrated to establish structured target pool;S500, effective compound for regulating blood-brain barrier permeability is screened out by molecular docking and primary cell verification, the application constructs gene-protein-metabolite network by multi-omics integration and cross-omics analysis, accurately identifies blood-brain barrier key target, provides high credibility target pool;Combined with molecular docking and primary cell mixed culture verification, realize efficient screening and functional safety evaluation of candidate compound, significantly reduce research and development cost, provide efficient technical path for drug research and development.

The invention relates to a multi-mode eye-brain collaborative maintenance intelligent device, and belongs to the technical field of eye-brain nursing equipment.The device is provided with an impression stimulation module, the impression stimulation module stimulates mild energy of an impression acupoint, the mild energy can be directly conducted to a paranasal sinus olfactory bulb in the deep part of a nasal cavity, the area is the starting end of paranasal sinus nerves, the paranasal sinus nerves are branches of cranial nerves, and therefore the paranasal sinus acupoint can be stimulated; the neural pathway directly extends to brain hippocampus, brain marginal tissue, pituitary gland and other core structures related to neuronutrition and brain metabolism; after stimulation, the paranasal sinus olfactory bulb and paranasal sinus nerves are activated to form a neural pathway for targeted delivery of the atomized liquid, so that the atomized particles of the brain care liquid are adsorbed on the surface of the paranasal sinus olfactory bulb and permeate into the brain along the activated neural pathway, and meanwhile, the permeability of the blood brain barrier is improved, so that the delivery resistance of the atomized liquid to related functional areas of the brain is further reduced, and the targeted delivery of the atomized liquid is realized. Precise nutrition for cranial nerves is achieved, and the problem that brain maintenance is invalid due to the fact that conventional nasal cavity atomized liquid enters the lung is thoroughly solved.

Many drugs and other molecules cannot ordinarily traverse the blood brain barrier (BBB). However, when alternating electric fields at certain optimum frequencies (e.g., 100 kHz) are applied to the brain, the BBB becomes permeable to those molecules. When the alternating electric fields are discontinued, the BBB eventually recovers to its original impermeable state. Subsequently, re-application of alternating electric fields at the optimum frequencies will re-open the BBB. Ordinarily, many frequencies (e.g., 200 kHz) of alternating electric fields are either ineffective or less effective at inducing permeability than the optimum frequencies. But once the BBB has been opened by applying alternating electric fields at the optimum frequencies, alternating electric fields at non-optimum frequencies can maintain the permeability of a previously-opened BBB.

The invention belongs to the field of treatment of inflammatory diseases, and provides application of an EED inhibitor in preparation of a medicine for preventing and treating blood-brain barrier permeability injury caused by endotoxemia. Experiments show that the EED inhibitor significantly reduces blood brain barrier permeability, inhibits neuroinflammation and improves disease behaviors. The invention provides a new medicine and a new way for treating secondary diseases related to endotoxemia.

The present invention relates to novel blood-brain barrier (BBB) ​​permeable substituted bicyclic derivatives capable of selectively inhibiting myosin light chain phosphate phosphorylation mediated by ROCK2 and / or Rho kinase, compositions comprising such derivatives, methods for preparing such derivatives and / or compositions, and methods for using such derivatives and / or compositions.

The invention discloses application of electroshock therapy in improvement of depression by regulating blood brain barrier and inflammatory factors, and relates to the technical field of nerve regulation. The invention discloses application of an electroshock treatment device in preparation of a medicine or a medical preparation for improving depression. The medicine or the medical preparation is used for improving depression by adjusting blood-brain barrier permeability of a subject. The regulation of the permeability of the blood-brain barrier comprises a close connection structure for repairing the blood-brain barrier; the repair is realized by up-regulating the expression of a tight junction protein, and the tight junction protein is selected from at least one of ZO-1, occuldin or claudin-5. The invention systematically discloses a brand new mechanism for treating and improving depression by electroshock, namely, a continuous path for reversing peripheral immune activation, repairing blood-brain barrier injury and relieving central nervous inflammation plays a role; the mechanism chain is clear, and innovative explanation is provided for the curative effect of ECT.

Described herein are methods for stimulating specific groups of neurons in the brain, e.g. the ventral tegmental area (VTA) or its axons, wherein the stimulation can open the blood-brain barrier (BBB) with sub-millimeter and / or sub-second specificity. Stimulation of the VTA can result in opening of the BBB for drug delivery, and / or opening the BBB for clearance of “junk” and / or waste. Also described herein, are non-invasive methods to provide control to BBB permeability.

By applying an alternating electric field to the brain for a period of time, certain substances (e.g., macromolecules) that would normally be unable to cross the blood-brain barrier can be introduced into the brain, where the frequency of the alternating electric field is chosen such that application of the alternating electric field increases the permeability of the blood-brain barrier. In some embodiments, the frequency of the alternating electric field is less than 190 kHz (e.g., 100 kHz). Once the permeability of the blood-brain barrier is increased, the substance is able to cross the blood-brain barrier.

The present invention addresses the problem of providing: a blood-brain barrier model that can be produced in a relatively simple manner and in which a physiological barrier function and selective permeability are reproduced; and a method for producing the blood-brain barrier model.　A blood-brain barrier model in which a physiological barrier function and selective permeability are reproduced can be produced in a relatively simple manner in a blood-brain barrier model having three cell layers of perisites, astrocytes, and vascular endothelial cells by culturing vascular endothelial cells so as to form a sheet structure on the surface of a porous membrane and so that perisites are present in an internal structure layer of the porous membrane.

This application relates to the field of intelligent healthcare, specifically to a method, device, and program product for assessing vascular occlusion reperfusion therapy based on thrombus permeability. It includes acquiring brain images of patients with acute ischemic stroke; classifying the responsible vessel in the brain images to determine whether it is large vessel occlusion, medium vessel occlusion, or distal small vessel occlusion; when the determination result is medium vessel occlusion, thrombus region localization and measurement are performed on the brain images, thrombus permeability is calculated, and reperfusion therapy is assessed based on the thrombus permeability to obtain an assessment result for intravenous thrombolysis or endovascular therapy. This application has significant clinical application value.

Examples are directed to devices, methods, and systems for determining opening or increased permeability of a blood-brain barrier (BBB) of a patient. An example device comprises memory circuitry that stores a set of non-transitory instructions, and processor circuitry coupled to the memory circuitry and configured to execute the instructions to determine opening or increased permeability of a BBB of a patient during an ultrasound-based BBB opening procedure using physiologic signals obtained from the patient.

When the composition of the present invention is used, it is possible to provide accurate basic information on the regulation of blood-brain barrier permeability, which limits future drug delivery, by predicting the permeability of the blood-brain barrier. In addition, when the pharmaceutical composition of the present invention is used, it is possible to effectively prevent, ameliorate or treat neuroinflammation by reducing blood-brain barrier permeability.

The invention discloses a bionic nano-carrier capable of reversibly regulating and controlling a blood brain barrier and application of the bionic nano-carrier. The bionic nano-carrier is of a core-shell structure, a lecithin surface modified inorganic nano-material serves as a core, a cell membrane of brain metastatic cancer cells coats the surface of the core to serve as a shell, and an A2A adenosine receptor agonist is loaded in a cell membrane coating layer. The blood brain barrier crossing efficiency is further improved; wherein the lecithin surface modified inorganic nano-material is obtained by using an oleic acid modified inorganic nano-material as a matrix and carrying out surface modification through amphiphilic ligand lecithin. The bionic nano-carrier regulates and controls the blood-brain barrier permeability in a reversible mode, the problems that the brain transfer efficiency of a brain metastatic cell membrane bionic carrier through a cell bypass path is limited, potential safety hazards exist in a traditional blood-brain barrier permeability regulation and control open means and the like are solved, and a new strategy is provided for efficient brain delivery.

A method of inducing blood-brain barrier (BBB) opening for delivery of a non-BBB permeable substance to a brain of a subject includes the steps of: administering an ethyl alcohol at 0.01%-5% v / v and the non-BBB permeable substance via microcatheter to a target area of the brain leading from an intracerebral artery and inducing a homogeneous distribution of the non-BBB permeable substance in the target area. The ethyl alcohol reaches the target area to induce a transient and reversible BBB opening for about 120 minutes so that the non-BBB permeable substance can penetrate the BBB to reach the target area. The non-BBB substance includes a therapeutic agent, a diagnostic agent, or a prophylactic agent. The use of low concentrations of ethyl alcohol allows the BBB to be restored to normal without causing significant harmful effects.

The present application relates to a fusion protein capable of permeating the blood-brain barrier and uses thereof, and provides a fusion protein capable of permeating the blood-brain barrier, a polynucleotide encoding the fusion protein, a vector containing the polynucleotide, a cell line transfected with the vector, and a pharmaceutical composition for preventing or treating diseases associated with brain dysfunction, comprising the fusion protein as an active ingredient.

When treating a subject using alternating electric fields (e.g., using TTFields to treat a tumor, or using alternating electric fields to increase the permeability of the blood brain barrier), some subjects experience an unpleasant electrosensation effect. This electrosensation can be reduced or eliminated by increasing the area of the transducer arrays that is active during certain times in the treatment. In some embodiments, this is accomplished by applying an AC signal between two pairs of transducer arrays at certain times (as opposed to the prior art approach of applying an AC signal between two individual transducer arrays).

By applying an alternating electric field to the brain for a period of time, certain substances (e.g., macromolecules) that would normally be unable to cross the blood-brain barrier can be introduced into the brain, where the frequency of the alternating electric field is chosen such that application of the alternating electric field increases the permeability of the blood-brain barrier. In some embodiments, the frequency of the alternating electric field is less than 190 kHz (e.g., 100 kHz). Once the permeability of the blood-brain barrier is increased, the substance is able to cross the blood-brain barrier.