Application of nanogold in preparation of reagent for measuring blood-brain barrier permeability

A blood-brain barrier and nano-gold technology is applied in the measurement of color/spectral properties, preparations for in vivo tests, and pharmaceutical formulations, to achieve the effects of simple raw materials, easy detection, and convenient and fast operation.

Active Publication Date: 2014-05-21
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are more than a dozen current mainstream methods for measuring the permeability of the blood-b

Method used

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  • Application of nanogold in preparation of reagent for measuring blood-brain barrier permeability
  • Application of nanogold in preparation of reagent for measuring blood-brain barrier permeability
  • Application of nanogold in preparation of reagent for measuring blood-brain barrier permeability

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Embodiment 1

[0023] At room temperature, dissolve the chloroauric acid crystals in double-distilled water to prepare 100ml of chloroauric acid solution with a concentration of 0.01%, heat to 90°C and keep for 10 minutes; quickly add 5.0ml, 1.5ml, 1.0ml, 0.60ml , 0.42ml, 0.32ml, 0.25ml of 1% trisodium citrate aqueous solution, stirred while heating for 30 minutes, and the stirring speed was set at 200 rpm to obtain a nano-gold solution; after filtration, the nano-gold solution was heated at 8000 rpm , centrifuged at 4°C, discarded the supernatant, dissolved in 100ml of double-distilled water and centrifuged at 8000 rpm at 4°C, repeated three times, and finally made up to 100ml with double-distilled water. The gold nanoparticles in Table 1 were obtained. Use the Malvern nano particle size analyzer to detect the particle size of gold nanoparticles, see figure 1 .

[0024] Seven groups (n=6) of male Sprague-Dawley rats (body weight 250-300g) and one group (n=6) of normal control Sprague-Dawl...

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Abstract

The invention provides the application of nanogold in preparation of a reagent for measuring blood-brain barrier permeability. The nanogold is formed by the following nanogold particles with the particle sizes by weight percent: 1 part of nanogold particles with the particle size range of 9-15nm and the average particle size of 11+/-0.5nm, 1 part of nanogold particles with the particle size range of 18-31nm and the average particle size of 27.5+/-0.5nm, 1 part of nanogold particles with the particle size range of 41-63nm and the average particle size of 48.5+/-0.5nm, 1 part of nanogold particles with the particle size range of 50-76nm and the average particle size of 68+/-0.5nm, 1 part of nanogold particles with the particle size range of 93-114nm and the average particle size of 104+/-0.5nm, 1 part of nanogold particles with the particle size range of 134-156nm and the average particle size of 145.5+/-0.5nm and 1 part of nanogold particles with the particle size range of 151-176nm and the average particle size of 166+/-0.5nm. According to the application, the quantitative measurement for the blood-brain barrier permeability of animals with cerebral trauma can be completed by the nanogold particles with different particle sizes; the number of nanogold permeating into the blood-brain barrier can be measured, and the largest pore of the opening of the blood-brain barrier can be estimated according to the particle sizes of the nanogold permeating into the blood-brain barrier; therefore, a quantitative measurement standard is provided for various blood-brain barrier damage models in animal experiments.

Description

technical field [0001] The invention belongs to the field of material measurement, and in particular relates to the application of nano gold in the preparation of reagents for measuring blood-brain barrier (Blood Brain Barrier, BBB) permeability. Background technique [0002] The blood-brain barrier is the main physiological barrier between human and animal blood and brain tissue. Under normal conditions, the opening degree of the blood-brain barrier is only a few nanometers, but under pathological conditions (especially various craniocerebral injuries), the blood-brain barrier may open up to tens or even hundreds of nanometers, which will lead to a series of successive diseases. Brain damage. [0003] The permeability of the blood-brain barrier greatly affects the prognosis of various traumatic brain injury models. In neuroscience experimental research at home and abroad, the detection of the blood-brain barrier permeability of experimental animals is an important experim...

Claims

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Application Information

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IPC IPC(8): A61K49/00B22F9/24G01N21/33
Inventor 谭亮李飞胡荣李文龑冯华
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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