Preparation method of drug microcarrier for acquired deafness based on microfluidic technology

A microfluidic technology and microcarrier technology are applied in the field of preparation of drug microcarriers, which can solve the problems of increasing the risk of tympanic membrane perforation and middle ear infection, limiting the efficacy of hearing loss, and the loss of the eustachian tube of drugs, and reducing the permanent tympanic membrane. Risk of sexual perforation and infection, improved drug therapy, less systemic side effects

Inactive Publication Date: 2021-11-02
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Traditional tympanic injection injects commonly used drugs such as dexamethasone and triamcinolone acetonide into the middle ear cavity, but the drugs are easily lost through the Eustachian tube
Due to the short residence time of drugs in the middle ear cavity, it is difficult to cross the round window membrane into the inner ear, resulting in low drug rates
In addition, drugs lost through the eustachian tube are often absorbed by the body through the digestive tract, resulting in greater side effects
In order to solve the problem of drug loss, it is necessary to keep the head in a special position for a long time, and this method has poor patient compliance
In addition, repeated intratympanic injections increase the risk of tympanic membrane perforation and middle ear infection
[0004] Therefore, the traditional method of directly injecting drugs through the tympanic cavity limits the efficacy of drugs for the treatment of hearing loss.

Method used

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  • Preparation method of drug microcarrier for acquired deafness based on microfluidic technology
  • Preparation method of drug microcarrier for acquired deafness based on microfluidic technology
  • Preparation method of drug microcarrier for acquired deafness based on microfluidic technology

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preparation example Construction

[0046] (1) Preparation steps of microfluidic loading:

[0047] The glass capillary was drawn using a tube drawing machine, the tube opening was ground to a thickness below 50 μm by sandpaper, soaked in alcohol, and cleaned ultrasonically. Glass capillary microfluidic chip was assembled with drawn glass capillary, glass capillary with outer diameter of 1000 μm, 300 μm, 100 μm, glass slide, sampling needle and quick-drying adhesive.

[0048] (2) The preparation steps of drug microcarrier:

[0049] According to the hydrophilicity and hydrophobicity of hearing protection drugs and adjuvants, high biosafety and degradable materials with the same hydrophilicity and hydrophobicity are formulated into polymerizable polymer prepolymer solutions, and a certain proportion of hearing protection drugs and adjuvants are mixed. An appropriate proportion of ultraviolet photoinitiator HMPP can be added to the prepolymerization solution; choose a solution with good biological safety and incomp...

Embodiment 1

[0055] Preparation of methyl methacrylate gelatin (Dexsp-GelMA) microcarrier loaded with dexamethasone sodium phosphate-saponin and its antagonism against cisplatin-induced hair cell damage:

[0056] 1. Preparation of methyl methacrylate gelatin (Dexsp-GelMA) microcarrier loaded with dexamethasone sodium phosphate-saponin

[0057] (1) Fabrication of a single-emulsion microfluidic chip: a glass capillary was drawn using a tube puller, and the nozzle was ground to a size below 50 μm by sandpaper, soaked in alcohol, and cleaned ultrasonically. Glass capillary microfluidic chip was assembled with drawn glass capillary, glass capillary with outer diameter of 1000 μm, 300 μm, 100 μm, glass slide, sampling needle and quick-drying adhesive.

[0058] (2) Preparation of single emulsion template: see figure 1 , prepare each solution, 50mg dexamethasone sodium phosphate and 130ug saponin are dissolved in the mixed aqueous solution of 1ml 8% (w / v) GelMA and 6% (v / v) HMPP (phenylacetone) ...

Embodiment 2

[0073] Preparation of N-acetyl-L-cysteine-loaded poly(lactic-co-glycolic acid) (PLGA) microcarriers and its antagonism against cisplatin-induced hair cell damage by microfluidic technology:

[0074] 1. Preparation of polylactic-co-glycolic acid (PLGA) microcarriers of dexamethasone.

[0075] (1) Fabrication of a single-emulsion microfluidic chip: a single-emulsion microfluidic chip meeting the requirements was fabricated, and the steps were the same as in Example 1.

[0076] (2) Preparation of single emulsion template: prepare each phase solution, dissolve dexamethasone in polylactic acid-glycolic acid copolymer (PLGA) methanol and methylene chloride solution as the internal phase, and the external phase is 2wt% PVA solution, respectively Connect the Teflon tube through the syringe to the two-phase inlet of the microfluidic chip, and control the flow rate of the peristaltic pump to shear the two-phase fluid into monodisperse single-emulsion droplets with a particle size of les...

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Abstract

The invention relates to a preparation method of a drug microcarrier for acquired deafness based on a microfluidic technology, which comprises the following steps of: controlling the generation of internal and external two-phase fluid by utilizing the micro-fluidic technology; the internal and external two-phase fluid generation process comprises the following steps: S1, preparing micron-scale liquid drops capable of being used for tympanic injection by utilizing shearing force and interfacial tension between two-phase fluids which are mutually insoluble, and in-situ entrapping hearing protective drugs and an auxiliary agent for increasing the permeability of a round window membrane; and S2, solidifying the liquid drops through polymerization reaction to form the drug microcarrier. Compared with the prior art, the prepared medicine microcarrier can be used for treating acquired deafness through tympanic injection, the middle ear cavity is filled with the medicine microcarrier through tympanic injection, the medicine is slowly released through natural degradation of the microcarrier and enters the inner ear through the round window membrane, and the effect of treating acquired deafness is achieved; the drug microcarrier has the advantages of small side effect and injury, lasting effect, obvious effect, simple operation and the like.

Description

technical field [0001] The invention relates to the field of biological materials and translational medicine, in particular to a method for preparing a drug microcarrier for acquired deafness based on microfluidic technology. Background technique [0002] Tympanic injection is one of the commonly used treatments for acquired deafness. Intratympanic injection of drugs can increase the local drug concentration in the inner ear, improve the drug absorption rate, reduce the systemic side effects of protective drugs such as dexamethasone, and reduce the impact of drugs on the antitumor properties of cisplatin. It has unique advantages and is a safe and effective treatment. [0003] Traditional tympanic injection injects commonly used drugs such as dexamethasone and triamcinolone acetonide into the middle ear cavity, but the drugs are easily lost through the Eustachian tube. Due to the short residence time of the drug in the middle ear cavity, it is difficult to cross the round ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/34A61K47/42A61K31/661A61K31/573A61K31/198A61P27/16
CPCA61K9/1647A61K9/1658A61K9/0046A61K31/661A61K31/573A61K31/198A61P27/16
Inventor 商珞然王家莉汪巧王武庆舒易来
Owner FUDAN UNIV
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