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Establishment method and application of novel immunological hepatitis model

A method for establishing a method and immunity technology, applied in the field of preparation of animal disease models, can solve the problems of lack of animal models, unable to cover the physiological and pathological characteristics of diseases, and no immune hepatitis animal models, etc. High, well-correlated effects

Inactive Publication Date: 2014-07-16
CHINA JILIANG UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Although the autoantibodies of immune hepatitis have been clarified, its etiology and the mechanism of liver cell damage have not been fully elucidated. One of the main reasons is the lack of an ideal animal model that can simulate the process of chronic progressive immune injury
However, the existing animal models of immune hepatitis cannot cover the physiological and pathological characteristics of the corresponding disease, only some of the clinical symptoms are similar, or some pathological changes are similar, and there is no standard animal model of immune hepatitis available

Method used

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Examples

Experimental program
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Embodiment 1

[0008] Example 1: Preparation and evaluation of immune hepatitis model

[0009] 1 Prepare the following materials:

[0010] 1.1 Experimental animals: ICR mice and C57BL / 6 mice were provided by Zhejiang Experimental Animal Center.

[0011] 1.2 Mice transfected with the whole genome of hepatitis B virus: Provided by Shanghai Southern Model Animal Center.

[0012] 1.3 Lipopolysaccharide: product of SIGMA Company of the United States.

[0013] 2 The preparation method is as follows:

[0014] 2.1 The method of perfusion was used to separate and purify the whole genome of hepatitis B virus transfected mouse hepatocytes.

[0015] 2.2 The isolated hepatocytes were suspended in phosphate buffer, ultrasonically crushed, and thoroughly mixed with Freund's complete adjuvant at a ratio of 1:1 to form a white emulsion, that is, an immune inducer.

[0016] 2.3 Clean-grade ICR mice or C57BL / 6 mice, half male and half male, were reared routinely.

[0017] 2.4 Take 6-week-old mice and inje...

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Abstract

The invention relates to preparation method for an immunological hepatitis animal model induced by hepatitis B virus whole genome transfected mouse hepatocytes, especially to an immunological inducer containing hepatocyte autoantigens and hepatitis B virus antigens and a simple operation procedure, generation of an immunological hepatitis disease model more similar to human diseases and application of the model in hepatitis pathogenesis study and anti-hepatitis drug evaluation.

Description

technical field [0001] The present invention relates to a preparation method of an animal disease model, in particular to a simple immune inducer and a simple operating procedure to produce an immune hepatitis disease model more similar to human diseases and its role in the study of the pathogenesis of hepatitis and the anti-inflammatory Applications in hepatitis drug evaluation. Background technique [0002] Immune hepatitis is a group of chronic immune liver diseases mediated by abnormal immunity and with similar clinical manifestations. It is an important cause of liver failure and cirrhosis. In recent years, the detection rate of immune hepatitis has gradually increased, accounting for 10% to 20% of chronic hepatitis worldwide. There are many causes involved in immune hepatitis, and the pathogenesis is very complicated. Immunological cell damage mechanism is one of the important mechanisms of liver injury. "Immune injury" is currently considered to be the main pathogen...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027
Inventor 伍义行沈钰明贾袁媛张虹莉戴灵豪
Owner CHINA JILIANG UNIV
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