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Chemiluminescence method for qualitative and quantitative detection of hepatitis B virus

A quantitative detection method, hepatitis B virus technology, applied in the field of clinical blood immune detection, can solve the problems of complex detection equipment, short storage period, narrow detection range, etc., and achieve the effect of large detection range, high precision and high accuracy

Inactive Publication Date: 2007-05-16
AUTOBIO DIAGNOSTICS CO LTD
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AI Technical Summary

Problems solved by technology

However, radioactive markers must be used in the radioimmunoassay method, and the detection equipment is complicated. It is necessary to use a special radioactive detector to measure the detection results, and there are also radioactive pollution and injuries to the operators.
At the same time, commonly used nuclides with short half-lives such as iodine-125, iodine-131 and phosphorus-32 have a short storage period, which also brings inconvenience to clinical use.
Although the enzyme immunoassay avoids the pollution, cumbersomeness, and short storage period of the radioimmunoassay, it still cannot meet the clinical needs due to its shortcomings such as narrow detection range and low sensitivity.

Method used

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  • Chemiluminescence method for qualitative and quantitative detection of hepatitis B virus

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Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Embodiment, (1), the preparation of hepatitis B virus surface antigen (HBsAg) quantitative detection kit:

[0038] 1. Coating plate production: use PH9.6 0.05M carbonate (CB) buffer as the coating buffer, add surface antigen 4ug / ml, coat according to 100ul / well, and overnight at 4°C after 3 hours at 37°C , then shake off the liquid, wash with washing solution, use 1% bovine serum albumin-phosphate (BSA-PBS)-2.5% sucrose (0.01 M, pH=7.2) as the blocking solution, and block overnight at 4°C.

[0039] 2. Antibody labeling:

[0040] 1) Dissolve 12 mg of horseradish peroxidase in 3 ml of freshly prepared deionized water.

[0041] 2) Add 0.1mol / L sodium periodate solution to 51ul / 1mg enzyme, mix well, and activate at 4°C for 60 minutes.

[0042] 3) Add ethylene glycol at 26ul / 1mg of enzyme, mix thoroughly, react at 4°C for 30 minutes, and terminate the reaction.

[0043] 4) Put the above reaction mixture into a dialysis bag, dialyze against 1 mmol / L acetate buffer, stay ov...

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Abstract

This invention relates to hepatitis B virus chemical light qualitative and quantitative test method, which tests hepatitis B surface antigen through its HBsAg and tests hepatitis B surface antibody through antibody HBs chemical test agent case and tests hepatitis B virus antigen through hepatitis antigen HBeAg test agent case and tests hepatitis virus e antibody through virus e antibody chemical property and tests hepatitis virus core antibody through virus core antibody chemical property.

Description

technical field [0001] The invention belongs to the technical field of clinical blood immune detection methods, and relates to a qualitative and quantitative detection method of hepatitis B virus by chemiluminescence. Background technique [0002] Hepatitis B is a worldwide infectious disease caused by hepatitis B virus (HBV). my country is a high prevalence area, and the national population infection rate is about 60%. According to the incidence rate of infectious diseases in my country's disease detection points, it is estimated that the annual number of hepatitis cases in the country reaches 2.69 million cases, of which patients aged 15-40 account for 63.1%. According to statistics, the number of patients suffering from hepatitis in my country is about 30 million, of which more than 60% are chronic hepatitis B patients. The five hepatitis B items are currently the most commonly used indicators of HBV infection. The detection of the five items is helpful for early detect...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/577G01N33/543G01N21/76
Inventor 付光宇李桂林李彬张学东黄晓马建军吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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