Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0133] Example 1: Determination of ammonia (ammonia ion) content in plasma
[0134] 1) Preparation of standard samples
[0135] Dissolve a certain amount of ammonia salt in water or buffer, and then adjust the ammonia concentration to 100 micromol / l;
[0136] 2) Pretreatment of the sample to be tested
[0137] The plasma sample is used as the sample to be tested, without pretreatment;
[0138] 3) Blank sample
[0139] The water or buffer is used as a blank sample, and its ammonia concentration is 0 micromol / L;
[0140] B. Preparation of reagent solution:
[0141] The ammonia diagnostic / measurement reagent in this embodiment is a single-dose reagent, which contains:
[0142] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0143] Glycerin 1mol / L
[0144] NADP + 0.25mmol / L
[0145] Aminokinase 16000U / L
[0146] Biotin carboxylase 18000U / L
[0147] Fatty acid synthase 12000U / L
[0148] Adenosine triphosphate 5mmol / L
[0149] Carboxybiotin-carboxy-carry...
Embodiment 2
[0165] Example 2: Determination of the content of ammonia (ammonia ion) in plasma
[0166] 1) Preparation of standard samples
[0167] Dissolve a certain amount of ammonia salt in water, then adjust the concentration of ammonia (ammonia ion) to 100 micromol / l, as a standard sample;
[0168] 2) Preparation of samples to be tested
[0169] The plasma sample is used as the sample to be tested, without pretreatment;
[0170] 3) Blank sample
[0171] The water is used as a blank sample, and its ammonia (ammonia ion) concentration is 0 micromol / L;
[0172] B. Preparation of reagent solution:
[0173] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0174] Reagent 1 consisting of:
[0175] Phosphate buffer 100mmol / L
[0176] NAD + 0.25mmol / L
[0177] Adenosine triphosphate 5mmol / L
[0178] Carboxybiotin-carboxy-carrying protein 5mmol / L
[0179] Monovalent phosphate 3mmol / L
[0180] Long chain fatty acid 4mmol / L
[0181] Coenzy...
Embodiment 3
[0204] Example 3: Determination of ammonia (ammonia ion) content in plasma samples
[0205] The operation mode of this embodiment is the same as that of embodiment 2, except that this embodiment:
[0206] B. Preparation of reagent solution:
[0207] Ammonia (ammonia ion) diagnostic / determination reagent is a three-agent reagent, which contains:
[0208] Reagent 1 consisting of:
[0209] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0210] NAD + 0.25mmol / L
[0211] Adenosine triphosphate 5mmol / L
[0212] Carboxybiotin-carboxy-carrying protein 5mmol / L
[0213] Monovalent phosphate 3mmol / L
[0214] Long chain fatty acid 4mmol / L
[0215] Coenzyme A 4mmol / L;
[0216] Reagent 2 consisting of:
[0217] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0218] Sodium chloride 2mol / L
[0219] Biotin carboxylase 28000U / L
[0220] Fatty acid synthase 50000U / L;
[0221] Reagent 3 consisting of:
[0222] Tris(carboxymethyl)aminomethane-hydro...
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