Ammonia (ammonia ion) determination method and ammonia (ammonia ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food inspection and determination, and can solve problems such as undiscovered blood ammonia determination methods
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Embodiment 1
[0126] Example 1: Determination of ammonia (ammonia ion) content in plasma
[0127] 1) Preparation of standard samples
[0128] Dissolve a certain amount of ammonia salt in water or buffer, and then adjust the ammonia concentration to 100 micromol / l;
[0129] 2) Pretreatment of the sample to be tested
[0130] The plasma sample is used as the sample to be tested, without pretreatment;
[0131] 3) Blank sample
[0132] The water or buffer is used as a blank sample, and its ammonia concentration is 0 micromol / L;
[0133] B. Preparation of reagent solution:
[0134] The ammonia diagnostic / measurement reagent in this embodiment is a single-dose reagent, which contains:
[0135] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0136] Glycerin 1mol / L
[0137] NADPH 0.25mmol / L
[0138] Aminokinase 16000U / L
[0139] Pyruvate carboxylase 18000U / L
[0140] Formate dehydrogenase 12000U / L
[0141] Adenosine triphosphate 5mmol / L
[0142] Oxaloacetic acid 5mmol / L
[0143] Monovalent phosphate...
Embodiment 2
[0157] Example 2: Determination of the content of ammonia (ammonia ion) in plasma
[0158] 1) Preparation of standard samples
[0159] Dissolve a certain amount of ammonia salt in water, then adjust the concentration of ammonia (ammonia ion) to 100 micromol / l, as a standard sample;
[0160] 2) Preparation of samples to be tested
[0161] The plasma sample is used as the sample to be tested, without pretreatment;
[0162] 3) Blank sample
[0163] The water is used as a blank sample, and its ammonia (ammonia ion) concentration is 0 micromol / L;
[0164] B. Preparation of reagent solution:
[0165] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0166] Reagent 1 consisting of:
[0167] Phosphate buffer 100mmol / L
[0168] NADH 0.25mmol / L
[0169] Adenosine triphosphate 5mmol / L
[0170] Oxaloacetic acid 5mmol / L;
[0171] Reagent 2 consisting of:
[0172] Phosphate buffer 100mmol / L
[0173] Ethylene glycol 5mol / L
[0174] Aminokinase 16000U / L
[0175] Pyruvat...
Embodiment 3
[0193] Example 3: Determination of ammonia (ammonia ion) content in plasma samples
[0194] The operation mode of this embodiment is the same as that of embodiment 2, except that this embodiment:
[0195] B. Preparation of reagent solution:
[0196] Ammonia (ammonia ion) diagnostic / determination reagent is a three-agent reagent, which contains:
[0197] Reagent 1 consisting of:
[0198] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0199] NADH 0.25mmol / L
[0200] Adenosine triphosphate 6mmol / L
[0201] Oxaloacetic acid 5mmol / L
[0202] Monovalent phosphate 5mmol / L;
[0203] Reagent 2 consisting of:
[0204] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0205] Sodium chloride 2mol / L
[0206] Pyruvate carboxylase 28000U / L
[0207] Formic acid dehydrogenase 50000U / L;
[0208] Reagent 3 consisting of:
[0209] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0210] Sodium chloride 2mol / L
[0211] Ammonia kinase 26000U / L.
[0212] Add these...
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