99 results about "Enzyme-linked immunosorbant assay" patented technology

The invention provides methods and systems for detecting a biomarker in a synovial fluid wherein the system also includes a control to ensure that the test sample is indeed synovial fluid. The biomarkers and the control for synovial fluid can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay.

The invention relates to a method for the determination of a cancer diagnostic/therapeutic biomarker assay and drug-targets including the following steps: (a) identification of potential candidate protein/peptide biomarkers and drug-targets based on the measurement of protein/peptide constituent concentrations in tissue sample proteomes as well as serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy non-human mammalian individuals as well as from cancerous non-human mammalian individuals and qualitatively selecting as potential candidate protein/peptide biomarkers those which show a pronounced differential behaviour between healthy and cancerous sample proteomes; (b) optional verification of the potential candidate protein/peptide biomarkers as identified in step (a) by quantitative mass spectrometric measurement of the potential candidate protein biomarkers in serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy non-human mammalian individuals as well as from cancerous non-human mammalian individuals and selecting as candidate protein/peptide biomarkers those which show a mass-spectrometrically measurable quantitative differential behaviour between healthy and cancerous sample proteomes; (c) validation of the candidate protein/peptide biomarkers as identified in step (a), or as optionally verified in step (b), by mass spectrometric measurement and/or antibody-based assays such as an Enzyme-Linked Immunosorbent Assay (ELISA) determination of the candidate protein biomarkers in serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy human individuals as well as from cancerous human individuals and selecting as protein/peptide biomarkers those which show a mass-spectrometrically measurable and/or antibody-based assay detectable differential behaviour between healthy and cancerous sample proteomes; (d) application of statistical methods to uncover single or groups of protein/peptide biomarkers as validated in step (c) as signatures for the detection of patients with cancer. The invention furthermore relates to specific biomarker assays for the highly reliable diagnosis of cancer, specifically of localized or non-localized prostate cancer, using human serum, plasma or any other derivatives of blood, or blood itself.

The invention discloses a rolling circle amplification DNA enzyme and covalent organic framework-based electrochemical-ELISA immunosensor, and belongs to the technical field of electrochemical detection. An electrochemical method and enzyme-linked immunosorbent assay are combined, a nucleic acid signal is amplified on an ELISA plate, an electrode is modified with a functional nanometer material, linear dependence between an electrical signal of a reduction peak and aflatoxin M1 within a range being 0.5-80ng/mL is generated, an association coefficient is 0.993, and the detection limit is 0.15ng/mL (S/N=3). By the rolling circle amplification DNA enzyme and covalent organic framework-based electrochemical-ELISA immunosensor, the electrode passivation in traditional electrochemical analysis can be prevented, an ELISA signal also can be effectively amplified, amplification of a cascading signal is achieved, the detection limit is reduced, trace detection is achieved, and a high-sensitivity, high-selectivity and high-throughput electrochemical ELISA method is built.

The invention discloses acetamiprid hapten and a preparation method thereof as well as application. The acetamiprid hapten is characterized in that the acetamiprid hapten is obtained by reacting N-noracetamiprid and aminobutyric acid; and the acetamiprid hapten is obtained by coupling acetamiprid hapten and carrier protein. The hapten prepared by the preparation method shows a specific acetamipridantigen determinant, so that a high-specificity acetamiprid monoclonal antibody is possibly screened out. The generated antibody is high in specificity and sensitivity, can be used for establishing an enzyme linked immunoadsorption assay method and a colloidal gold test paper quick measuring method, so that quick detection on acetamiprid in tobaccos and food is realized.

The present invention concerns novel synthetic blocking reagents for the reduction of non-specific bindings in solid phase assays that rely on biological and specific recognition, e.g., in enzyme-linked immunosorbent assays (ELISAs). The invention provides the use of compounds as blocking reagents as well as kits comprising these compounds. The compounds comprise one or more poly(ethylene glycol) moieties, one or more alkyl- or aminoalkyl groups and another unit bridging the aforementioned groups, wherein the compound comprises at least one amino group.

The invention discloses preparation and an enzyme-linked immunosorbent assay method for heavy metal mercury polyclonal antibody in the technical field of heavy metal detection. The polyclonal antibody is prepared for the enzyme-linked immunosorbent assay by the following steps of: bonding the heavy metal mercury at one end of penicillin G sodium serving as a dual-functional chelating agent; coupling the other end with high molecular carrier proteins, namely bovine serum albumin and ovalbumin, to form immunogen MMPA-BSA and envelope antigen MMPA-OVA of mercury-chelating agent-protein; and finally emulsifying and immunizing the immunogen MMPA-BSA to prepare the polyclonal antibody. The prepared antibody has high specificity for the mercury ions, a cross reaction ratio with all other metals of less than 0.001 percent apart from the cross reaction ratio with cadmium of 7.9 percent and an adding recovery ratio of between 91.4 and 120 percent; and thus the antibody can be used for the assayof the heavy metal mercury in a water sample and can also be developed into rapid immunological detection technology for rapidly detecting the heavy metal mercury in other samples such as agricultural products and the like by combining certain pre-processing technology.

The present invention relates to methods and systems for testing for the presence of a material such as one or more analyte types within a sample and more particularly, for improved single enzyme-linked immunosorbent assay (sELISA) testing as well as other variants of single-enzyme linked molecular analysis (SELMA). The invention involves flow systems for digital counting of analytes with at least one opening (inlet/outlet). A support with hydrophilic and hydrophobic patches preferably harbours capture probes immobilised on the hydrophilic features. Nano-to-attoliter droplets are formed on the hydrophilic features. A gas phase (called gas phase seal) is applied to prevent/reduce evaporation from the droplets.

The invention provides preparation methods and application of pendimethalin hapten and antigen. The pendimethalin hapten is characterized by being prepared by taking N-(1-ethylpropyl)-3,4-dimethylaniline and 4-ethyl bromobutyrate to react to generate alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, taking the alkylated N-(1-ethylpropyl)-3,4-dimethylaniline and concentrated nitric acid to react togenerate nitro-alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, and then reacting with potassium hydroxide; the pendimethalin antigen is prepared by coupling the pendimethalin hapten and carrier protein. The antigen prepared by the invention has a specific pendimethalin antigen determinant, so that a high-specificity pendimethalin monoclonal antibody is possibly screened. The produced antibody hashigh specificity and high sensitivity and can be used for establishing an enzyme-linked immunosorbent assay method and a colloidal gold test strip determination method, so that rapid detection of pendimethalin in tobaccos and foods is realized.

The present invention concerns the development of a quantitative method for the molecular diagnosis of autosomal recessive spinal muscular atrophy (SMA) by measuring the amount of cytosolic mRNA from human muscle cells. Both the procedure using radioactive material and the Enzyme-Linked Immunosorbent Assay (ELISA) nonradioactive method were developed using 32P-dCTP labeled and biotinylated nucleotide probes. The results obtained demonstrate that the measurement of mRNA could be used as a quantitative method for the molecular diagnosis of SMA. There was a perfect concordance of the results obtained between the procedure using radioactive material, the ELISA method and the single strand conformation polymorphism (SSCP) analysis regarding the negative and positive SMA samples. The methods developed in this study may be applicable to the diagnosis (detection of homozygous and heterozygous deletions in exons 7 and 8 of the SMN gene) and the control of mRNA concentrations in the future gene therapy of patients with SMA.

The invention discloses a kind of enzyme linked immuno-sorbent assay reagent box applying to the analysis of the Carboruran residue, and it includes the box body, the 96-hole/40-hole enzyme marker board placed in the box body and the reagent placed in the box body, and its characteristic lies in that in each hole of the enzyme marker board is the envelope antigen enveloped by the envelope liquid and able to combined and react with the anti-Carboruran antibody specioficity and sealed with 1.0-3.0% nonfat dried milk, and that the reagent in the box includes cleaning mixture, substrate-matter diluents, Carboruran standard solution, anti-CArboruran antibody, horseradish peroxide enzyme marker sheep anti-rabbit antibody, substrate matter, color-displaying matter and reaction-termiinated liquor.

The present invention provides a method for the preparation of ready-to-use solid support for ELISA for rapid identification and quantitative estimation of protein/antigen in the test samples, and performances of the assay itself. The invention also provides for a quick, accurate and stable estimation of protein/antigen in the test samples. The invention also provides an ELISA kit comprising of ready-to-use solid support along with wash buffers, chemical substrate, substrate buffer, stock solution, and positive and negative control samples.

Raw material-2-methyl-4-chlorophenoxy carboxylic acid (2-ME-4-CHL) is acrylated by sulfoxide chloride, then reacting with amino-butyric and amino-caproic acid respectively, to prepare half antigens: 4-(2-methyl-4-chlorophenoxyacetyl) amino butyric acid and 6-(2-methyl-4-chlorophenoxyacetyl) amino caproic acid (MB and MC for short, resp). And 2-ME-A-CHL itself can be half-antigen (M for short). Halfantigens M, MB and MC are coupled with protein to produce immune antigen and envelope antigen by carbodi-imide and mixed acid anhydride methods. Immue antigen immune animals are used to produce special antibody with high avidity to 2-ME-4-CHL. This antibody does not react with other compounds. Enzyme-linked immunosorbant assay established with said antibody can test trace ammount of 2-ME-4-CHL residues quickly and conveniently.

The invention discloses a Neuroglobin enzyme-linked immunosorbent assay kit and the use of it. The Neuroglobin enzyme-linked immunosorbent assay kit provided by the invention includes Neuroglobin polyclonal antibody, Neuroglobin monoclonal antibody and enzyme labeled antibody. Neuroglobin monoclonal antibody is produced through culturing hybridoma cells prepared by cell conjugation after inoculating mice with Neuroglobin antigen, whereas Neuroglobin polyclonal antibody is produced from animal antiserum after inoculating with Neuroglobin antigen.

The present invention provides a rapid, highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), referred to as N-Assay, a device, and a kit, for detection and identification of microorganisms in a sample, in thirty minutes or less, with little or no interference from non-target microorganisms. The present invention also provides for simultaneous determination of antimicrobial susceptibility of microorganism in the N-Assay.

The invention provides a preparation method and application of a polyclonal antibody of a rice stripe virus nucleocapsid protein, and belongs to the technical field of rice stripe virus detection. Thepolyclonal antibody of the rice stripe virus nucleocapsid protein CP is obtained by immunizing animals with the rice stripe virus nucleocapsid protein CP as immunogen; and the amino acid sequence ofthe rice stripe virus nucleocapsid protein CP is as shown in SEQ ID No. 1. The polyclonal antibody of the rice stripe virus nucleocapsid protein has the characteristics of low cost, wide application range, multiple audiences, high sensitivity, and the like, and can be used for multiple biological detection experiments such as an immune spot determination method, an enzyme-linked immunosorbent assay method, a protein immunoblotting method, an immuno-fluorescence method, an immuno-electron microscopy method, and the like. By utilizing the disclosed polyclonal antibody, disease early warning is carried out through small brown rice plant hoppers at the beginning of outbreak of rice stripe disease, and multi-means disease monitoring is facilitated.

The invention discloses a preparation method and application of a quinclorac hapten and antigen. The method is characterized in that the quinclorac hapten is prepared by using quinclorac and (1,3-dioxan-2-ylethyl)magnesium bromide for reaction to generate aldolactal quinclorac which is then reacted with trifluoroacetic acid; the quinclorac antigen is obtained by coupling the quinclorac hapten andcarrier protein. The antigen prepared through the method presents a specific quinclorac antigenic determinantantigen epitope, so that screening out a high-specificity quinclorac monoclonal antibody becomes possible; the generated antibody is high in specificity and high in sensitivity, and can be used for building an enzyme-linked immunosorbent assay method and a colloidal gold test strip rapid determination method, so that quinclorac in food and tobacco-planting soil is fast detected.

The Assay Analysis, Tracking and Reporting (AATR) is a software system to acquire and analyse Enzyme-Linked ImmunoSorbant Assays (ELISA) assays for the purspose of Antibody Screening, Detection, Concentration, Tracking, Dose Respone, Adsorption Analysis and Reporting using second/third/fourth degree polynomial, 4 and 5 Parameter Logistics best curve fitting regression analysis. AATR provides mechanism and capabilities to acquire data directly from Microplate Reader devices including batch mode and allows for import/export of ELISA data and sample ids for the tray. The regression analysis uses positive controls and negative controls and multiple standards data points. An average is calcuated for the duplicate samples. The software automatcialy color codes the wells and sample ids in the plate for the outliers data. Additional color coding and marker are used to designate the wells/samples above and below the highest and lowest thresold. AATR allows exporting of graphs, data points and reports in various formats.

A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.

The present invention relates to: the use of a strain of Brucella spp. expressing the green fluorescent protein (GFP) in the preparation of medicaments for the prevention of brucellosis in mammals, vaccines against brucellosis, and the method for identifying mammals to which said vaccines have been administered. Preferably, the method of the invention is an immunoassay, and, more preferably, it is an enzyme-linked immunosorbent assay (ELISA).

The invention discloses a method for detecting heavy metal content in tea, and relates to the technical field of detection of heavy metal in food. The method comprises the following steps of: crushingthe tea to be detected, adding into water, and performing ultrasonic treatment; adding a heavy metal flocculant to precipitate precipitation, adding glycosidase to the precipitation, and performing enzymatic hydrolysis of the precipitation in a water bath until precipitation is completely dissolved, so as to obtain a to-be-detected liquid; and detecting the content of lead, chromium, cadmium, andcopper of the to-be-detected liquid by enzyme linked immunosorbant assay. The method for detecting the heavy metal content in the tea obtains the to-be-detected liquid containing no acid by optimizedthe pretreatment method, is safety and environmental friendliness, and can be used for the enzyme linked immunosorbant assay.

The invention discloses an application of angiopoietin-like protein 7 in prognosis evaluation of heart failure. When a serum sample of a heart failure patient is detected by using an enzyme-linked immunosorbent assay method, and the content of the angiopoietin-like protein 7 in the sample is greater than or equal to 869 pg/mL, the patient has a high probability of poor prognosis, i.e., events of 30-day death and 90-day death can occur at a large probability. According to the application, the angiopoietin-like protein 7 is used as a marker for prognosis evaluation of heart failure, and the prognosis condition of the heart failure patient can be judged by detecting the concentration of the ANGPTL7 in serum. Through predicting the prognosis condition of the heart failure patient by utilizingthe marker, the application is simple and easy to implement and precise in result, has a guiding effect on a clinical treatment scheme, has good application advancement and value, and is worthy of vigorous popularization and protection.

This invention relates to a kind of potato virus Y (PVY) yunnan outlier three antibody sandwich enzyme immune adsorbing rule (TAS-ELISA) detection kit and its preparation method. The kit comprises positive and negative contrast, PVY multi-clone antibody, PVY single clone antibody, enzyme standard antibody and other materials and medicines. The PVY multi-clone antibody is got from rabbit immune adsorbing blood serum after separately purifying the PVY yunnan outlier. The PVY single clone antibody is got from mouse immune, cell fusion, clone and purification after separately purifying the PVY yunnan outlier. The detection sensitivity of the TAS-ELISA detection kit in this invention can reach 0.01ng/ml. It coincides with the comparing accuracy rate of electrical glass detection result, and it is 100 times than indirect ELISA and DAS-ELISA detection sensitivity.

The invention mainly relates to a classical swine fever virus Erns protein affinity polypeptide and application. The polypeptide is designed by a computer-simulated virtual molecular docking technology, and the sequence is WRHYIH. According to the invention, a polypeptide sequence P1 is obtained according to a virtual molecular screening technology by taking a swine fever Erns protein crystal structure obtained by homologous modeling as a template. After the polypeptide is artificially synthesized, an enzyme-linked immunosorbent assay test and a plasma resonance test are employed to identify the affinity of the interaction of the sequence and the swine fever Erns protein, and the result shows that the sequence P1 and the swine fever Erns protein have relatively high specificity and affinity; and the invention provides a reliable theoretical basis for affinity chromatography taking affinity peptide as an affinity ligand, and swine fever Erns protein can be rapidly detected by labeling the polypeptide.