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Compositions and methods to treat muscular & cardiovascular disorders

A composition and cardiovascular technology, which can be used in cardiovascular system diseases, biochemical equipment and methods, muscular system diseases, etc., and can solve problems such as unsatisfactory effective treatment.

Inactive Publication Date: 2012-07-25
NOVARTIS AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is an ongoing unmet need for effective treatments for diseases or conditions that may be caused by dysregulated microRNAs

Method used

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  • Compositions and methods to treat muscular & cardiovascular disorders
  • Compositions and methods to treat muscular & cardiovascular disorders
  • Compositions and methods to treat muscular & cardiovascular disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0160] Identification and characterization of miRNA-208-2

[0161] Small RNA expression profiles were profiled according to the following protocol:

[0162] RNA isolation

[0163] After deep anesthesia with isoflurane (3%, 20 L / min), rats and mice were subjected to partial thoracotomy and perfusion through the left ventricle. The left ventricle was punctured with a 23-gauge needle from an airfoil perfusion device (SV-19BLK; Termudo, Elkton, MD), which was attached to a wash solution (NaCl 0.9% and 250,000 U / l heparin at 38°C) Closed pressurized syringe. The right atrium was punctured to provide outflow, and the perfusate was infused at a precisely controlled pressure of 120 mm Hg. Perfusion was continued for 2 minutes (20 ml) at a constant rate. Organs were isolated, snap frozen in liquid nitrogen and stored at -80°C. According to the manufacturer's instructions, in 1 ml per 100 mg of tissue Organs were homogenized using a polytron homogenizer in the presence of Reagent...

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Abstract

The present invention relates to a novel microRNA, mir-208-2, implicated in muscular and cardiovascular disorders. The present invention also relates to oligonucleotide therapeutic agents (antisense oligonucleotides and / or double stranded oligonucleotides such as dsRNA) and their use in the treatment of muscular and cardiovascular disorders resulting from dysregulation of mir-208-2.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 200780046435.8 (PCT / US2007 / 025535) with an application date of December 13, 2007 and the invention title is "Composition and Method for Treating Muscle and Cardiovascular Disorders". Field of Invention [0002] The present invention relates to a novel small RNA (microRNA), mir-208-2, involved in muscle and cardiovascular disorders. The present invention also relates to oligonucleotide therapeutics (antisense oligonucleotides and / or double-stranded oligonucleotides) and their use in the treatment of muscular and cardiovascular disorders resulting from mir-208-2 dysregulation. Background technique [0003] Small RNAs (miRNAs) are a class of non-coding RNA genes whose final product is a functional RNA molecule of about 22 nt. They are single-stranded RNAs processed from endogenously encoded incomplete hairpin precursors. They appear to act via translational inhibition by base pairing to the 3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/63C12N5/10A61K48/00A61P21/00A61P9/00C12Q1/68
CPCC12N15/113C12N2310/113C12N2310/141A61P9/00A61P9/02A61P9/06A61P9/10A61P21/00A61P21/04A61P43/00A61K48/00
Inventor I·伯文克J·哈尔J·魏勒C·施内尔M·米勒M·申克-布劳恩F·塞卢卡
Owner NOVARTIS AG