Suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and preparation method thereof

A detection kit and suspension chip technology, which is applied in the field of suspension chip detection kit and its preparation for the diagnosis of extrapelvic metastases of ovarian cancer before surgery, to achieve the effect of eliminating subjectivity

Inactive Publication Date: 2012-07-25
广西壮族自治区肿瘤防治研究所
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an efficient, fast, accurate, non-invasive, sensitive, specific, and low-cost suspension chip detection kit for diagnosing extrapelvic metastases of preoperative ovarian cancer and its preparation method to overcome the current There are many deficiencies in the detection rate of ovarian cancer preoperative pelvic, peritoneal and other implanted metastases

Method used

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  • Suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and preparation method thereof
  • Suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and preparation method thereof
  • Suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and preparation method thereof

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Embodiment Construction

[0022] Suspension chip detection kit and its preparation method for diagnosis of extrapelvic metastasis of ovarian cancer before operation

[0023] 1. Experimental materials

[0024] Matrix Metalloproteinases 9 (MMP9), Heparanases 1 (Hpa1), cathepsin L (CL) three full-length encoded amino acid mouse anti-human monoclonal antibodies and Matrix Metalloproteinases 9 (MMP9, C-terminus), Heparanases 1 (Hpa1, C-terminus ), cathepsin L (CL C-terminus) three goat anti-human polyclonal antibodies were purchased from Santa Cruz Biotechnology, Inc.

[0025] The multicolor microspheres used are the coupled hydroxyl fluorescent microspheres of Luminex Company.

[0026] Streptavidin-PE was from Innovabiosciences, UK.

[0027] 2. Preparation of the kit

[0028] Preparation of Fluorescent Microsphere Suspension Chip

[0029] Three kinds of coupled hydroxyl fluorescent microspheres were respectively coated with mouse anti-human monoclonal antibodies of three full-length cDNA-encoded proteins...

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Abstract

The invention discloses a suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and a preparation method thereof. The kit mainly comprises three kinds of coupling hydroxyl fluorescent microspheres and three kinds of goat anti-human polyclonal antibodies, wherein the three kinds of coupling hydroxyl fluorescent microspheres respectively coat three kinds of murine anti-human monoclonal antibodies of full-length cDNA (complementary DNA) coding protein, i.e., MMP9 (matrix metalloproteinase-9), Hpa1 and CL; and the three kinds of goat anti-human polyclonal antibodies respectively are streptavidin and mycin-phycoerythrobilin affinity coupling MMP9, Hpa1 and CL goat anti-human polyclonal antibodies. The kit adopts a suspension chip technology to realize joint detection of the content of three kinds of extracellular matrix proteases MMP9, Hpa1 and CL. The kit can be used for diagnosing preoperative pelvic external transfer of ovarian cancer patients and contributes to determining the best therapeutic schedule and judging the prognosis condition and the like.

Description

technical field [0001] The invention relates to a suspension chip detection kit in the field of medical biotechnology, in particular to a suspension chip detection kit for diagnosing extrapelvic metastases of preoperative ovarian cancer and a preparation method thereof. Background technique [0002] Ovarian malignancy is a common gynecological malignancy both at home and abroad. The mortality rate ranks first among gynecological malignancies, and the five-year survival rate is only about 30%. One of the important reasons for the high mortality rate is that ovarian cancer is prone to early invasion and metastasis, and 70% of the patients are already in the advanced stage when they are discovered. The treatment of ovarian cancer depends on factors such as the nature of the tumor, histological type, surgical-pathological stage, and the age of the patient to determine whether to perform adjuvant therapy after surgery. Most patients with early epithelial ovarian cancer without o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 李力王琪张玮
Owner 广西壮族自治区肿瘤防治研究所
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