Suspension chip detection kit used for diagnosing preoperative ovarian cancer pelvic external transfer and preparation method thereof
A detection kit and suspension chip technology, which is applied in the field of suspension chip detection kit and its preparation for the diagnosis of extrapelvic metastases of ovarian cancer before surgery, to achieve the effect of eliminating subjectivity
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[0022] Suspension chip detection kit and its preparation method for diagnosis of extrapelvic metastasis of ovarian cancer before operation
[0023] 1. Experimental materials
[0024] Matrix Metalloproteinases 9 (MMP9), Heparanases 1 (Hpa1), cathepsin L (CL) three full-length encoded amino acid mouse anti-human monoclonal antibodies and Matrix Metalloproteinases 9 (MMP9, C-terminus), Heparanases 1 (Hpa1, C-terminus ), cathepsin L (CL C-terminus) three goat anti-human polyclonal antibodies were purchased from Santa Cruz Biotechnology, Inc.
[0025] The multicolor microspheres used are the coupled hydroxyl fluorescent microspheres of Luminex Company.
[0026] Streptavidin-PE was from Innovabiosciences, UK.
[0027] 2. Preparation of the kit
[0028] Preparation of Fluorescent Microsphere Suspension Chip
[0029] Three kinds of coupled hydroxyl fluorescent microspheres were respectively coated with mouse anti-human monoclonal antibodies of three full-length cDNA-encoded proteins...
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